《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

迷走神经电刺激对脑外伤昏迷大鼠前额叶皮质5-羟色胺2A受体表达的影响①

董晓阳，冯珍   

  1. 南昌大学第一附属医院康复医学科，江西南昌市 330006。
  • 出版日期:2016-04-25 发布日期:2016-06-01

Effect of Vagus Nerve Stimulation on Expression of 5-hydroxytryptamine 2A Receptor in Prefrontal Cortex of Coma Rats with Traumatic Brain Injury

DONG Xiao-yang, FENG Zhen   

  1. Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China
  • Published:2016-04-25 Online:2016-06-01

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摘要： 目的 探讨迷走神经电刺激对脑外伤昏迷大鼠促醒作用及对前额叶皮质 5-HT2A受体表达的影响。方法 将 72 只Sprague-Dawley大鼠随机分为空白对照组、假刺激组、刺激组和拮抗剂组，每组 18只。应用自由落体撞击法建立脑外伤昏迷模型。拮抗剂组注入OXR1受体拮抗剂SB334867，采用迷走神经电刺激治疗刺激组和拮抗剂组。观察其行为学变化，并用免疫组织化学技术检测各组大鼠前额叶皮质 5-HT2A受体表达的影响。结果 刺激组 12 只苏醒，拮抗剂组 9 只苏醒，假刺激组 4 只苏醒。5-HT2A受体含量从低到高依次为：空白对照组、拮抗剂组、假刺激组、刺激组(χ2=11.464, P=0.009)。结论 迷走神经电刺激可提高脑外伤昏迷大鼠意识状态，其机制可能与上调5-HT2A受体有关。

关键词: 脑外伤, 昏迷, 迷走神经电刺激, 5-羟色胺2A受体, 食欲素A, 大鼠

Abstract: Objective To explore the effect of vagus nerve stimualtion on wake-promoting and the expression of 5-hydroxytryptamine (5-HT) 2A receptor in the prefrontal contex of coma rats with traumatic brain injury. Methods 72 Sprague-Dawley rats were randomly divided into control group, sham-stimulated group, stimulated group and antagonist group with 18 rats in each group. Traumatic brain injury model was established by a weight-drop head injury. The antagonist group was injected with SB334867, and both the antagonist group and the stimulated group received vagus nerve stimulation. Their behaviors were recorded. And immunohistochemistry technique was used to detect the expression of 5-HT2A receptor in the prefrontal cortex. Results 12 rats in the stimulated group, 9 in the antagonist group and 4 in the sham-stimulated woke up. The expression of 5-HT2A receptor from low to high was ranged as the control group, the antagonist group, the sham-stimulated group and the stimulated group (χ2=11.464, P=0.009). Conclusion Vagus nerve stimulation could raise consciousness in coma rats after traumatic brain injury, which may be related to up-regulating the expression of 5-HT2A receptor.

Key words: traumatic brain injury, coma, vagus nerve stumulation, 5-hydroxytryptamine 2A receptor, orexin-A, rats