《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2008, Vol. 14 ›› Issue (05): 442-444.
• 基础研究 • Previous Articles Next Articles
HU Li-ting, ZHAO Gui-qiu, DU Zhao-dong, et al
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Abstract: Objective To observe the effect of small-interfering RNA(siRNA) targeting c-Met,the receptor of hepatocyte growth factor(HGF),on the proliferation of human lens epithelial cells(LECs).Methods siRNA was transferred into LECs cultured in vitro by HiperFect Transfection Reagent.Real-Time PCR was applied to observe the expression of c-Met mRNA in LECs after gene transfer,and MTT assay was used to detect the proliferation of LECs induced by HGF.Results The expression of c-Met mRNA in LECs was significantly decreased in the experimental group,compared to that in the controls(P<0.01).Proliferation of LECs induced by HGF was inhibited,compared with the single HGF stimulated group(P<0.01).Conclusion The RNA interference targeting c-Met can effectively inhibit the expression of c-Met mRNA,and the proliferation of LECs induced by HGF.
Key words: lens, epithelial cells, hepatocyte growth factor(HGF), c-Met, small-interfering RNA(siRNA)
HU Li-ting;ZHAO Gui-qiu;DU Zhao-dong;et al. RNA Interference Targeting c-Met Inhibits Proliferation of Lens Epithelial Cells [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2008, 14(05): 442-444.
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