血清二胺氧化酶含量与大鼠急性脊髓损伤后肠道运动功能的关系

doi:10.3969/j.issn.1006-9771.2017.06.003

Abstract

Abstract: Objective To measure the level of diamine oxidase (DAO), and observe the intestinal motor and mucosal barrier injury after acute spinal cord injury (SCI) in rats. Methods A total of 45 Sprague-Dawley rats were randomly divided into SCI group (group A, n=15), sham group (group B, n=15) and control group (group C, n=15). SCI model was established with Allen's strike mode (10 g × 25 mm) by striking T₁₀ spinal segment in rats. One day, three days and seven days after SCI, hind limb motor function was assessed with Basso-Beattie-Bresnahan (BBB) Scale in each group, the myoelectric slow wave and ileum smooth muscle contractility were measured in rats, ileum tissues were tested with HE staining, and the DAO content of serum was tested with ELISA kit. Results One day, three days and seven days after SCI, the BBB score was significantly lower in group A than in groups B and C (P<0.001). One day, three days after SCI, the frequency and amplitude of both slow wave and contractility were lower in group A than in groups B and C (P<0.05); seven days after SCI, there was no significant difference among three groups (P>0.05). Group A showed ileal mucosal edema, lodging, inflammatory cell infiltration, and submucosal gap increase. The Chiu's score of intestinal mucosal injury was higher in group A than in groups B and C (P<0.05), as well as the serum DAO content one day and three days after SCI (P<0.05), and no significant difference was found in the serum DAO content among three groups seven days after SCI (P>0.05). Conclusion Serum DAO content may respond to the intestinal motor function and mucosal injury after acute SCI in rats.

Key words: spinal cord injury, diamine oxidase, intestinal motor function, mucosal injury, rats

CLC Number:

R651.2

YANG Sen, YANG Zheng, YAN Jing-xin, XU Zhou, XU Wen-jian, TIAN Hai-yan, DAI Xiao-lin, YANG Ping. Relationship between Serum Diamine Oxidase Content and Intestinal Motility after Acute Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(6): 634-640.

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Relationship between Serum Diamine Oxidase Content and Intestinal Motility after Acute Spinal Cord Injury

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