脉冲射频对坐骨神经慢性压迫大鼠降钙素基因相关肽表达的远期影响

doi:10.3969/j.issn.1006-9771.2018.05.007

Abstract

Abstract: Objective To investigate the long-term effect of pulsed radiofrequency on the expression of calcitonin gene-related peptide (CGRP) in dorsal root ganglion of rats with chronic constriction injury. Methods A total of 36 Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and treatment group (n=12). The sciatic nerve was ligated in the model group and the treatment group, and the treatment group was treated with pulsed radiofrequency on the injured nerve for 120 seconds. Hindpaw withdrawl threshold (HWT) was measured before treatment, and seven days, 14 days, 21 days and 28 days after treatment. After HWT, the right L_4-6 dorsal root ganglions were tested the CGRP expression using RT-qPCR and ELISA. Results HWT gradually increased in the treatment group after treatment, and was higher than that in the model group since seven days after treatment (P<0.05), similar to that of the sham group 21 days and 28 days after treatment (P>0.05). The transcription and translation levels of CGRP were both significantly lower in the treatment group than in the model group (P<0.01).Conclusion Pulsed radiofrequency might relieve the neuropathic pain through reducing CGRP expression in dorsal root ganglion in a long term.

Key words: neuropathic pain, sciatic nerve, pulsed radiofrequency, calcitonin gene-related peptide, rats

CLC Number:

R745.4

FU Miao, REN Hao, LUO Fang. Long-term Effect of Pulsed Radiofrequency on Expression of Calcitonin Gene-related Peptide in Chronic Constriction Injured Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(5): 526-529.

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Long-term Effect of Pulsed Radiofrequency on Expression of Calcitonin Gene-related Peptide in Chronic Constriction Injured Rats

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