运动训练对脑缺血再灌注大鼠认知功能及前额皮层神经元核抗原和突触素1表达的效果

doi:10.3969/j.issn.1006-9771.2021.11.006

Abstract

Abstract:

Objective To observe the effect of exercise on cognitive function and the expression of NeuN and SynapsinI in prefrontal cortex of rats after cerebral ischemia-reperfusion. Methods Forty health male Sprague-Dawley rats were randomly divided into sham group (S group, n = 10), model group (M group, n = 10), sham & exercise group (SE group, n = 10) and model & exercise group (ME group, n = 10). The left middle cerebral artery was occluded for one hour and reperfused. SE and ME groups accepted treadmill training, for 14 days. The cognitive function of rats was evaluated with open field experiment and new object recognition experiment. The number and distribution of nerve cells in rat brain were observed with Nissl staining and immunofluorescence staining. The SynapsinI expression in serum was detected with ELISA.Results Compared with S group, the activity, times of crossing, length and activity time in central area of open field experiment decreased in M group, as well as the cognitive index and length of new object recognition experiment (P < 0.05). Compared with M group, all the indexes increased in ME group (P < 0.05). The number of Nissl bodies decreased in M group (P < 0.05) and the arrangement was disordered, while the number of Nissl bodies increased in ME group (P < 0.05) compared with M group. The NeuN positive cells was more in S group than in M group (P < 0.05), and it was more in ME group than in M group (P < 0.05); as well as that of SynapsinI. The expression of SynapsinI decreased in M group (P < 0.05), and increased in ME group (P < 0.05). Most results of behavioral experiments positively correlated with the expression of Nissl, NeuN and SynapsinI (r > 0.221, P < 0.05).Conclusion Cerebral ischemia-reperfusion can cause cognitive impairment in rats. Exercise can alleviate nerve injury and improve cognitive function, which may relate to promoting the expression of NeuN and SynapsinI in prefrontal cortex, to increase the number of neurons and synapses.

Key words: ischemia-reperfusion, cognitive impairment, exercise, SynapsinI, NeuN, rats

LING Meng-yu,YANG Yi-zhuo,LIU Shuai,YE Chao-qun. Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(11): 1272-1281.

Figures/Tables 14

References 31

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运动方案	第1~3天	第4~10天	第11~17天
初运动	3 m/min, 10 s	3 m/min, 10 s	5 m/min, 10 s
一级运动	5 m/min, 20 s	8 m/min, 20 s	10 m/min, 20 s
二级运动	5 m/min, 5 min	12 m/min, 30 min	15 m/min, 30 min

组别	1 d	3 d	7 d	10 d	14 d	17 d	F值	P值
S组	0	0	0	0	0	0	0	1
SE组	0	0	0	0	0	0	0	1
M组	1.88±0.64	2.25±0.46	2.13±0.35	1.75±0.71	1.13±0.35	0.75±0.46	6.298	0.004
ME组	1.86±0.71	2.09±1.22	1.46±0.69	0.91±0.54	0.55±0.52	0.27±0.47	11.257	< 0.001
F值^a	0.056	0.121	6.318	8.687	7.341	4.871
P值^a	0.99	0.815	0.022	0.009	0.015	0.041

组别	n	术前	3 d	7 d	10 d	14 d	17 d	F值	P值
S组	10	215.4±3.6	234.7±6.1	260.5±5.4	282.3±7.7	312.3±11.6	343.4±14.2	145.358	< 0.001
SE组	10	216.7±3.1	232.3±4.8	268.4±6.4	288.3±8.7	318.5±10.5	349.0±11.5	195.322	< 0.001
M组	10	215.9±2.9	203.0±22.8^a	219.8±26.1^a	237.1±29.3^a	256.4±32.2^a	289.2±34.5^a	9.343	< 0.001
ME组	10	218.1±2.5	204.0±18.1	229.2±19.1	248.2±23.6^b	280.1±28.2^b	312.4±29.0^b	18.811	< 0.001
F值		0.739	9.961	14.996	10.781	9.452	9.475
P值		0.544	0.001	< 0.001	< 0.001	0.001	0.001

组别	n	穿格次数	总距离(mm)	中央区域活动时间(s)
S组	10	41.20±7.20	2136.40±432.15	3.92±1.08
SE组	10	41.60±8.23	2255.20±369.01	3.49±1.39
M组	10	22.60±7.16^a	935.40±116.35^a	0.14±0.22^a
ME组	10	22.80±8.01^b	926.60±142.052^b	0.15±0.26^b
F值		21.837	37.99	26.728
P值		< 0.001	< 0.001	< 0.001

组别	n	活跃度	穿格次数	总距离(mm)	中央区域活动时间(s)
S组	10	29.79±3.86	55.83±7.20	2788.56±433.86	4.89±1.24
SE组	10	43.14±7.10^a	58.17±9.82	2977.66±225.81^a	7.92±1.76^a
M组	10	12.71±2.55^a	23.83±11.60^a	1319.33±447.95^a	1.11±0.94^a
ME组	10	26.07±1.42^b	50.67±20.70^b	2746.01±616.79^b	4.06±1.41^b
F值		42.222	7.056	14.294	25.067
P值		< 0.001	0.002	< 0.001	< 0.001

Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion

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组别	n	CI	总距离(mm)
S组	10	0.68±0.04	10051.75±4094.99
SE组	10	0.80±0.04	15163.41±3226.99^a
M组	10	0.41±0.07^a	5077.68±1096.70^a
ME组	10	0.74±0.05^b	8839.58±895.08^b
F值		64.482	27.895
P值		< 0.001	< 0.001

组别	n	尼氏体计数
S组	10	78.00±6.04
SE组	10	88.4±6.50^a
M组	10	41.8±3.49^a
ME组	10	63.2±6.42^b
F值		61.80
P值		< 0.001

组别	n	NeuN	SynapsinI
S组	10	74.6±4.50	33.20±7.16
SE组	10	94.8±8.056.91^a	46.60±6.23^a
M组	10	53.4±4.76^a	16.8±5.22^a
ME组	10	64.6±7.20^b	47.00±7.68^b
F值		35.590	23.072
P值		< 0.001	< 0.001

组别	n	SynapsinI血清蛋白浓度
S组	10	166.90±6.58
SE组	10	187.35±22.90
M组	10	134.22±7.72^a
ME组	10	260.85±34.48^b
F值		31.734
P值		< 0.001

项目	穿格次数	总距离	中央区域活动时间	认知指数	尼氏体计数	NeuN	SynapsinI	血清SynapsinI
活跃度	0.611^a	0.731^c	0.882^c	0.830^c	0.855^c	0.221^b	0.720^b	0.362^c
穿格次数	-	0.932^c	0.622^b	0.725^c	0.547^a	0.060^a	0.721^c	0.548^a
总距离		-	0.664^b	0.836^c	0.651^b	0.620^a	0.831^c	0.603^b
中央区域活动时间			-	0.821^c	0.814^c	0.219	0.685^b	0.301^a
认知指数				-	0.835^c	0.580^a	0.891^c	0.613^b
尼氏体计数					-	0.338^b	0.631^a	0.270
NeuN						-	0.320^a	0.739^c
SynapsinI							-	0.761^c

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