隔日限食减轻脊髓损伤大鼠炎症反应的芳香烃受体/细胞因子信号传导抑制因子2/核转录因子-κB信号通路机制

doi:10.3969/j.issn.1006-9771.2022.05.009

Abstract

Abstract:

Objective To explore the role of aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB (AHR/SOCS2/NF-κB) signaling in the reduction of spinal cord injury (SCI) inflammation with every-other-day fasting (EODF). Methods A total of 36 healthy male Sprague-Dawley rats were randomly divided into sham group (n = 12), model group (n = 12) and EODF group (n = 12). The latter two groups were established C₅ spinal cord hemi-clamp model. The EODF group received EODF, namely fasting and feeding per 24 hours (water free), for two weeks after operation. They were assessed with Grooming Test before operation, and one, seven and 14 days after operationt, respectively. Pathological injury of spinal cord was observed with HE staining 14 days after operation, while the expression of AHR, SOCS2 and NF-κB proteins were detected with Western blotting, and the mRNA of AHR, SOCS2, inhibitor α of NF-κB (IκBα), NF-κB and tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) were measured with reverse transcription real-time quantitative polymerase chain reaction. Results Compared with the sham group, the Grooming Test score decreased in the other two groups (P < 0.05), and cavity, edema and structural disorder appeared in the spinal cord. Compared with the model group, the pathology of EODF group improved, and the Grooming Test score increased (P < 0.05). The mRNA and protein expression of AHR and SOCS2 increased (P < 0.05), the mRNA and protein expression of NF-κB decreased (P < 0.05), the mRNA expression of IκBα increased (P < 0.05), and the mRNA expression of TNF-α and IL-6 decreased (P < 0.05). Conclusion EODF may promote the recovery of motor function of forelimb in rats with SCI, and relieve damage and inflammation, which may associate with the activation of AHR/SOCS2/NF-κB signaling pathway.

Key words: spinal cord injury, inflammation, every-other-day fasting, aryl hydrocarbon receptor, suppressor of cytokine signaling 2, nuclear transcription factor-κB, rats

CLC Number:

R651.2

ZHOU Xiaojue,FENG Jing,PANG Rizhao,LIU Jie,ZHANG Anren. Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 544-551.

Figures/Tables 5

References 51

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组别	n	术前	术后1 d	术后7 d	术后14 d	F值	P值
假手术组	12	4.79±0.50	2.42±0.76	3.67±0.86	4.13±0.74	22.667	< 0.001
模型组	12	4.83±0.39	0.58±0.51^a	0.83±0.58^a	1.17±0.39^a	213.067	< 0.001
EODF组	12	4.58±0.51	0.50±0.52^a	0.92±0.67^a	1.67±0.49^a,b	132.724	< 0.001
F值		0.972	37.682	61.458	95.517
P值		0.389	< 0.001	< 0.001	< 0.001

组别	n	AHR	SOCS2	NF-κB	IκBα	TNF-α	IL-6
假手术组	4	1.231±0.237	1.265±0.463	1.000±0.302	1.951±0.488	0.865±0.103	0.475±0.031
模型组	4	0.576±0.207^a	0.502±0.015^a	1.887±0.464^a	1.054±0.424^a	1.575±0.120^a	0.853±0.136^a
EODF组	4	1.412±0.352^b	1.233±0.408^b	0.906±0.133^b	2.347±0.347^b	0.819±0.264^b	0.435±0.136^b
F值		10.380	5.857	10.840	9.771	22.760	16.770
P值		0.005	0.024	0.004	0.006	< 0.001	< 0.001

组别	n	AHR	SOCS2	NF-κB
假手术组	4	0.897±0.073	0.616±0.132	0.744±0.112
模型组	4	0.630±0.059^a	0.253±0.067^a	1.199±0.233^a
EODF组	4	0.961±0.189^b	0.723±0.282^b	0.857±0.144^b
F值		8.318	7.204	7.653
P值		0.009	0.014	0.011

Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway

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