氙气后处理对脊髓缺血再灌注损伤的效果：Akt信号通路和自噬机制

doi:10.3969/j.issn.1006-9771.2023.02.006

Abstract

Abstract:

Objective To investigate the effect of xenon post-conditioning on autophagy after spinal cord ischemia/reperfusion injury (SCIRI) in rats and its relationship with protein kinase B (Akt) signaling pathway. Methods A total of 30 male rats were randomized into sham-operated group (sham group), spinal cord ischemia/reperfusion injury group (I/R group) and I/R + xenon post-conditioning group (Xe group), with ten rats in each group. In the latter two groups, SCIRI was induced by clamping the abdominal aorta for 85 minutes followed by reperfusion for four hours. Xe group inhaled xenon and oxygen (1∶1) for one hour at one hour after initiation of reperfusion, while the other groups inhaled nitrogen and oxygen (1∶1) for one hour. After the reperfusion, they were assessed with Basso-Beattie-Bresnahan (BBB) scale and slanting board test. And then, their spinal cords of L_3-5 were obtained. Nissl staining was used to count the number of normal neurons. Western blotting was used to detect the protein expression of Akt, p-Akt, p62, Beclin 1, microtubule-associated protein 1 light chain 3 (LC3) Ⅰ, LC3 Ⅱ. The mRNA expression of Beclin 1, p62 and LC3 Ⅱ in the spinal cord was measured with reverse transcription real-time quantitative polymerase chain reaction. Results Compared with the sham group, the BBB score and the maximum inclination of the slanting board test decreased, the count of normal neurons decreased, the protein expression of p62 and the p-Akt/Akt ratio decreased (P< 0.01), the protein and mRNA expression of Beclin 1 and LC3 Ⅱ, and the LC3 Ⅱ/LC3 Ⅰ ratio increased, the p62 mRNA expression decreased (P< 0.01) in the I/R group. Compared with the I/R group, the BBB score and the maximum inclination of the slanting board test increased, the count of normal neurons increased, the protein expression of p-Akt and p62 increased, the p-Akt/Akt ratio increased, the protein and mRNA expression of Beclin 1, LC3 Ⅱ and LC3 Ⅱ/LC3 Ⅰ ratio decreased, and the mRNA expression of p62 increased (P< 0.01) in Xe group.

Conclusion Xenon post-conditioning may relieve SCIRI in rats, which is related to activating Akt signaling pathway to inhibit autophagy.

Keywords: spinal cord; ischemia/reperfusion; xenon post-conditioning; autophagy; protein kinase B; rats

CLC Number:

R651.2

LUO Lan, LI Lu, JIN Mu. Effect of xenon post-conditioning on spinal cord ischemia/reperfusion injury in rats: regulating Akt signaling pathway and autophagy[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(2): 174-181.

Figures/Tables 7

References 43

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组别	n	BBB评分	斜板试验最大倾斜度/°
Sham组	10	21.000±0.000	62.300±2.908
I/R组	10	5.243±3.236^a	41.800±4.492^a
Xe组	10	12.850±1.462^a,b	50.000±6.236^a,b
F值		144.172	47.301
P值		< 0.001	< 0.001

组别	n	正常神经元计数	p-Akt/Akt
Sham组	5	11.920±0.867	1.156±0.103
I/R组	5	5.560±0.573^a	0.781±0.84^a
Xe组	5	8.840±0.385^a,b	0.963±0.75^a,b
F值		123.564	27.218
P值		< 0.001	< 0.001

组别	n	p62/actin	Beclin 1/actin	LC3 Ⅱ/LC3 Ⅰ
Sham组	5	1.148±0.091	0.797±0.033	0.268±0.023
I/R组	5	0.710±0.640^a	1.214±0.426^a	0.521±0.011^a
Xe组	5	0.883±0.059^a,b	0.963±0.344^a,b	0.441±0.014^a,b
F值		55.396	161.836	292.112
P值		< 0.001	< 0.001	< 0.001

组别	n	p62	Beclin 1	LC3 Ⅱ
Sham组	5	1.003±0.901	1.003±0.094	1.013±0.174
I/R组	5	0.546±0.148^a	1.303±0.141^a	1.469±0.167^a
Xe组	5	0.886±0.681^b	1.131±0.169	1.181±0.245^c
F值		24.352	5.857	6.716
P值		< 0.001	0.017	0.011

Effect of xenon post-conditioning on spinal cord ischemia/reperfusion injury in rats: regulating Akt signaling pathway and autophagy

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