《中国康复理论与实践》

《中国康复理论与实践》

• 基础研究 • 上一篇    下一篇

小剂量超短波治疗对大鼠脊髓损伤后炎症反应及水肿的影响①

万峪岑，孙师，赵利娜，尹艳梅，冯智萍，周禹鑫，张志强，张立新   

  1. 中国医科大学附属盛京医院康复中心，辽宁沈阳市110022。
  • 出版日期:2016-02-25 发布日期:2016-06-01

Effects of Low Dose UltrashortWave Therapy on Inflammation and Edema after Spinal Cord Injury in Rats

WAN Yu-cen, SUN Shi, ZHAO Li-na, YIN Yan-mei, FENG Zhi-ping, ZHOU Yu-xin, ZHANG Zhi-qiang, ZHANG Li-xin   

  1. Department of Rehabilitation, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110022, China
  • Published:2016-02-25 Online:2016-06-01

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摘要： 目的观察小剂量超短波治疗对大鼠脊髓损伤后运动功能恢复、水肿及巨噬细胞反应的影响，并探讨其作用机制。方法56 只Sprague-Dawley 大鼠分为3 组。假手术组(n=8)暴露硬脊膜后，不予打击，直接缝合；模型组(n=24)采用Allen 打击法建立大鼠脊髓损伤(SCI)模型，不给予任何治疗；超短波组(n=24)在SCI 后第2 天开始给予受损部位小剂量超短波(最大输出功率40 W，实际输出功率11.58 W)治疗，7 min/次，1 次/d，至取材前。于造模后1 周、2 周、3 周、4 周采用BBB评分评定大鼠的后肢运动功能，免疫组织化学染色检测水通道蛋白-4(AQP-4)、巨噬细胞-小胶质细胞胞质抗原(ED-1)的表达。结果治疗后，BBB评分逐渐增加；术后1~4 周，与模型组相比，超短波组评分明显增加(t>3.368, P<0.01)。术后1 周起，模型组、超短波组的AQP-4 及ED-1 阳性表达较假手术组增加，且呈下降趋势；与模型组相比，超短波组各时间点的AQP-4 及ED-1 表达均减少(t>3.156, t>4.466, P<0.05)。结论脊髓损伤后超短波治疗可以减轻损伤处继发性水肿，减少炎症细胞的活化及浸润，促进神经功能恢复。

关键词: 脊髓损伤, 超短波, 水通道蛋白-4, 巨噬/小胶质细胞, 大鼠

Abstract: Objective To investigate the effect of ultrashort wave therapy on edema and inflammatory reaction after spinal cord injury (SCI) in rats. Methods 56 Sprague-Dawley rats were divided into sham- operated group (n=8), model group (n=24) and ultrashort wave group (n=24). The model was established with Allen's method. The sham-operated group was exposed endorhachis without hit. The ultrashort wave group was exposed to ultrashort wave radiation 7 minutes, once a day, 24 hours after modeling until the animals were sacrificed. Locomotors functional recovery was assessed every week post operation period by BBB score. Immunohistochemical staining was performed to observe the expression of the aquaporin-4(AQP-4) and ED-1. Results The BBB scores increased in the model group and the ultrashort wave group after treatment, and was higher in the ultrashort wave group than in the model group from 1 week after treatment (t>3.368, P<0.01). The expressions of AQP-4 and ED-1 were higher in the model group and ultrashort wave group than in the sham-operated group, and decreased as time went on. They were lower in the ultrashort wave group than in the model group(t>3.156, t>4.466, P<0.05). Conclusion Ultrashort wave therapy can alleviate the edema after SCI, reduce the activation and infiltration of inflammatory cells, and promote the recovery of neurological function.

Key words: spinal cord injury, ultrashort wave, aquaporin-4, macrophage/microglias, rats