双光子显微镜在小动物活体光学成像中的研究进展

doi:10.3969/j.issn.1006-9771.2017.01.009

Abstract

Abstract: Twophoton microscopy is a new technique which combines laser scanning con-focal microscopy and two-photon excitation technique. Two-photon fluorescence microscopy has the advantages of little light damage, small bleaching area, strong penetrability, high resolution, high fluorescence collection efficiency, and high image contrast. It is suitable for dark field imaging and multi-labeled compound measurement, and has been widely used in small animals in vivo optical imaging, such as research for tumour, gene therapy, stem cells, drug development, spinal cord injury, etc.

Key words: two-photon microscopy, in vivo optical imaging, small animals, review

CLC Number:

R446.8

ZHANG Wen-hao, LI Jian-jun, YANG De-gang, YANG Ming-liang, DU Liang-jie, GAO Feng, LIU Chang-bin, LI Da-peng, HU An-ming, CAI Chang. Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(1): 37-41.

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Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)

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