脂多糖对大鼠星形胶质细胞Ski蛋白表达的影响

doi:10.3969/j.issn.1006-9771.2017.05.005

Abstract

Abstract: Objective To explore how and where ski expresses under lipopolysaccharide (LPS) in rats' astrocytes. Methods Astrocytes were obtained from cerebral cortex of a newborn (within 3 days) Sprague-Dawley rat and cultured in vitro. Astrocytes were cultured with LPS in concentration of 0 μg/ml, 0.001 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 100 μg/ml for six hours; and cultured with LPS in concentration of 0.1 μg/ml for 0 day, 2 days, 4 days, 6 days and 8 days. The level of ski was determined with Western blotting, and the location of ski was detected with indirect immunofluorescent staining. Results The expression of ski was induced by LPS, especially in the concentration of 0.1 μg/ml. The expression of ski induced with 0.1 μg/ml LPS peaked at 4 days of inducement and then decreased. Ski was mainly observed in nuclear in the normal astrocytes and the astrocytes induced with 0.1 μg/ml LPS for 6 days. However, it was observed in cytoplasm 2 and 4 days of inducement. Conclusion LPS could induce the expression of ski in rats' astrocytes, which may participate in inflammation.

Key words: ski, lipopolysaccharide, inflammation, astrocyte, rats

CLC Number:

R364.5

ZHAO Xin, GUO Yong-qiang, WANG Ming, CHEN Tie-ge, WU Ya-min, ZHANG Hai-hong. Effect of Lipopolysaccharide on Ski Expression in Rats' Astrocytes[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(5): 514-519.

References

[1] Bonnon C, Atanasoski S. c-Ski in health and disease [J]. Cell Tissue Res, 2012, 347(1): 51-64.
[2] 周开升,朱彦东,张海鸿,等. Ski在神经系统中的作用及机制的研究进展[J]. 中国康复理论与实践, 2016, 22(7): 797-800.
[3] Pekny M, Wilhelmsson U, Pekna M. The dual role of astrocyte activation and reactive gliosis [J]. Neurosci Lett, 2014, 565: 30-38.
[4] Donnelly DJ, Popovich PG. Inflammation and its role in neuroprotection, axonal regeneration and functional recovery after spinal cord injury [J]. Exp Neurol, 2008, 209(2): 378-388.
[5] Lukovic D, Stojkovic M, Moreno-Manzano V, et al. Concise review: reactive astrocytes and stem cells in spinal cord injury: good guys or bad guys? [J]. Stem Cells, 2015, 33(4): 1036-1041.
[6] Fernandez AM, Fernandez S, Carrero P, et al. Calcineurin in reactive astrocytes plays a key role in the interplay between proinflammatory and anti-inflammatory signals [J]. J Neurosci, 2007, 27(33): 8745-8756.
[7] de Rivero Vaccari JP, Lotocki G, Marcillo AE, et al. A molecular platform in neurons regulates inflammation after spinal cord injury [J]. J Neurosci, 2008, 28(13): 3404-3414.
[8] Zhu P, Li JX, Fujino M, et al. Development and treatments of inflammatory cells and cytokines in spinal cord ischemia-reperfusion injury [J]. Mediators Inflamm, 2013, 2013: 701970.
[9] Li P, Liu P, Xiong RP, et al. Ski, a modulator of wound healing and scar formation in the rat skin and rabbit ear [J]. J Pathol, 2011, 223(5): 659-671.
[10] Catorce MN, Gevorkian G. LPS-induced murine neuroinflammation model: main features and suitability for pre-clinical assessment of nutraceuticals [J]. Curr Neuropharmacol, 2016, 14(2): 155-164.
[11] Ahuja CS, Fehlings M. Concise review: bridging the gap: novel neuroregenerative and neuroprotective strategies in spinal cord injury [J]. Stem Cells Transl Med, 2016, 5(7): 914-924.
[12] Oyinbo CA. Secondary injury mechanisms in traumatic spinal cord injury: a nugget of this multiply cascade [J]. Acta Neurobiol Exp (Wars), 2011, 71(2): 281-299.
[13] Stenudd M, Sabelström H, Frisén J. Role of endogenous neural stem cells in spinal cord injury and repair [J]. JAMA Neurol, 2015, 72(2): 235-237.
[14] Allison DJ, Ditor DS. Immune dysfunction and chronic inflammation following spinal cord injury [J]. Spinal Cord, 2015, 53(1): 14-18.
[15] Leal-Filho MB. Spinal cord injury: from inflammation to glial scar [J]. Surg Neurol Int, 2011, 2: 112.
[16] Trinchieri G, Sher A. Cooperation of Toll-like receptor signals in innate immune defence [J]. Nat Rev Immunol, 2007, 7(3): 179-190.
[17] Zhang X, Wang J, Qian W, et al. Dexmedetomidine inhibits tumor necrosis factor-alpha and interleukin 6 in lipopolysaccharide-stimulated astrocytes by suppression of c-Jun N-terminal kinases [J]. Inflammation, 2014, 37(3): 942-949.
[18] Heiman A, Pallottie A, Heary RF, et al. Toll-like receptors in central nervous system injury and disease: a focus on the spinal cord [J]. Brain Behav Immun, 2014, 42: 232-245.
[19] Dong Y, Benveniste EN. Immune function of astrocytes [J]. Glia, 2001, 36(2): 180-190.
[20] Biesmans S, Acton PD, Cotto C, et al. Effect of stress and peripheral immune activation on astrocyte activation in transgenic bioluminescent Gfap-luc mice [J]. Glia, 2015, 63(7): 1126-1137.
[21] Biesmans S, Meert TF, Bouwknecht JA, et al. Systemic immune activation leads to neuroinflammation and sickness behavior in mice [J]. Mediators Inflamm, 2013, 2013: 271359.
[22] Deheuninck J, Luo K. Ski and SnoN, potent negative regulators of TGF-beta signaling [J]. Cell Res, 2009, 19(1): 47-57.
[23] Boche D, Cunningham C, Docagne F, et al. TGFbeta1 regulates the inflammatory response during chronic neurodegeneration [J]. Neurobiol Dis, 2006, 22(3): 638-650.
[24] Zhou K, Nan W, Feng D, et al. Spatiotemporal expression of Ski after rat spinal cord injury [J]. Neuroreport, 2017, 28(3): 149-157.
[25] Ikeshima-Kataoka H. Neuroimmunological implications of AQP4 in astrocytes [J]. Int J Mol Sci, 2016, 17(8): E1306.
[26] Middeldorp J, Hol EM. GFAP in health and disease [J]. Prog Neurobiol, 2011, 93(3): 421-443.

Effect of Lipopolysaccharide on Ski Expression in Rats' Astrocytes

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