跑台运动对脑外伤大鼠基质金属蛋白酶-2表达的影响

doi:10.3969/j.issn.1006-9771.2017.05.007

Abstract

Abstract: Objective To determine the effect of treadmill exercise on expression of matrix metalloproteinase-2 (MMP-2) in rats following traumatic brain injury (TBI). Methods Fifty-five male adult Sprague-Dawley rats were randomly assigned to sham (n=15), control (n=20) and exercise (n=20) groups, the later two groups subjected to unilateral cortical contusion injury (CCI). All the rats were assessed with foot-fault test 6, 12, 18 and 24 days after CCI. Evans blue perfusion was used to evaluate the integrity of the blood-brain-barrier (BBB) 48 hours after CCI. Protein expression of MMP-2 was determined with Western blotting one week after CCI. Results The score of foot-fault test improved more in the exercise group than in the control group 12, 18 and 24 days after CCI (F>4.793, P<0.05). Evans blue extravasation was less in the exercise group than in the control group (t=-8.091, P<0.001), as well as the expression of MMP-2 (t=-13.12, P<0.001). Conclusion Early treadmill exercise can improve the motor function in rats with TBI, which may associate with inhibition of MMP-2 expression to protect BBB integrity.

Key words: traumatic brain injury, treadmill exercise, blood-brain-barrier, matrix metalloproteinase, rats

CLC Number:

R651.1

SHEN Xia-feng, TIAN Shan, WU Yi, ZHU Yu-lian. Effect of Treadmill Exercise on Expression of Matrix Metalloproteinase-2 in Rats with Traumatic Brain Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(5): 525-528.

References

[1] Sheriff FG, Hinson HE. Pathophysiology and clinical management of moderate and severe traumatic brain injury in the ICU [J]. Semin Neurol, 2015, 35(1): 42-49.
[2] Balu R. Inflammation and immune system activation after traumatic brain injury [J]. Curr Neurol Neurosci Rep, 2014, 14(10): 484.
[3] Mustafa AG, Alshboul OA. Pathophysiology of traumatic brain injury [J]. Neurosciences (Riyadh), 2013, 18(3): 222-234.
[4] Raghupathi R. Cell death mechanisms following traumatic brain injury [J]. Brain Pathol, 2004, 14: 215-222.
[5] Alluri H, Wiggins-Dohlvik K, Davis ML, et al. Blood-brain barrier dysfunction following traumatic brain injury [J]. Metab Brain Dis, 2015, 30(5): 1093-1104.
[6] Shlosberg D, Benifla M, Kaufer D, et al. Blood-brain barrier break down as a therapeutic target in traumatic brain injury [J]. Nat Rev Neurol, 2010, 6(7): 393-403.
[7] Shi H, Wang HL, Pu HJ, et al. Ethyl pyruvate protects against blood-brain-barrier damage and improves long-term neurological outcomes in a rat model of traumatic brain injury [J]. CNS Neurosci Ther, 2015, 21(4): 374-384.
[8] 刘翔,许静红,齐铁伟,等. 急性创伤性脑损伤大鼠血脑屏障通透性变化与Claudin-5的相关性研究[J]. 中华神经医学杂志,2012, 11(9): 954-957.
[9] 沈夏锋,于惠贤,吴军发,等.早期跑台训练在改善颅脑外伤大鼠认知功能中的应用[J]. 中国康复医学杂志, 2015, 30(2):112-116.
[10] Zhao Z, Sabirzhanov B, Wu J, et al. Voluntary exercise preconditioning activates multiple antiapoptotic mechanisms and improves neurological recovery after experimental traumatic brain injury [J]. J Neurotrauma, 2015, 32(17): 1347-1360.
[11] Archer T, Svensson K, Alricsson M. Physical exercise ameliorates deficits induced by traumatic brain injury [J]. Acta Neurol Scand, 2012, 125(5): 293-302.
[12] 李爱萍,沈夏锋,胡永善,等. 早期跑台训练对中重度颅脑外伤大鼠运动功能的影响[J]. 中华物理医学与康复杂志, 2013,35(4): 265-268.
[13] Metz GA, Whishaw IQ. Cortical and subcortical lesions impair skilled walking in the ladder rung walking test: a new task to evaluate fore- and hindlimb stepping, placing, and co-ordination [J]. J Neurosci Methods, 2002, 115(2): 169-179.
[14] Batra A, Latour LL, Ruetzler CA, et al. Increased plasma and tissue MMP levels are associated with BCSFB and BBB disruption evident on post-contrast flair after experimental stroke [J]. J Cereb Blood Flow Metab, 2010, 30(6): 1188-1199.
[15] 金卫篷,周源,王东,等. 阿托伐他汀对脑外伤小鼠血脑屏障的影响[J]. 中华实验外科杂志, 2015, 32(6): 1361-1364.
[16] Abdul-Muneer PM, Pfister BJ, Haorah J, et al. Role of matrix metalloproteinases in the pathogenesis of traumatic?brain injury [J]. Mol Neurobiol, 2016, 53(9): 6106-6123.
[17] Ding JY, Kreipke CW, Schafer P, et al. Synapse loss regulated by matrix metalloproteinases in traumatic brain injury is associated with hypoxia inducible factor-1alpha expression [J]. Brain Res Bull, 2009, 1268: 125-134.
[18] Abdul-Muneer PM, Conte AA, Haldar D, et al. Traumatic brain injury induced matrix metalloproteinase 2 cleaves CXCL12α (stromal cell derived factor 1α) and causes neurodegeneration [J]. Brain Behav Immun, 2017, 59: 190-199.
[19] Zhang S, Kojic L, Tsang M, et al. Distinct roles for metalloproteinases during traumatic brain injury [J]. Neurochem Int, 2016, 96: 46-55.
[20] 郑克彬,刘艳峰,刘海鹏,等. 基质金属蛋白酶家族在颅脑损伤患者中表达水平的变化[J]. 中华实验外科杂志, 2015, 32(1): 178-179.
[21] Roberts DJ, Jenne CN, Léger C, et al. Association between the cerebral inflammatory and matrix metalloproteinase responses after severe traumatic brain injury in humans [J]. J Neurotrauma, 2013, 30(20): 1727-1736.
[22] Copin JC, Rebetez MM, Turck N, et al. Matrix metalloproteinase 9 and cellular fibronectin plasma concentrations are predictors of the composite endpoint of length of stay and death in the intensive care unit after severe traumatic brain injury [J]. Scand J Trauma Resusc Emerg Med, 2012, 20: 83.
[23] Liu CL, Chen CC, Lee HC, et al. Matrix metalloproteinase-9 in the ventricular cerebrospinal fluid correlated with the prognosis of traumatic brain injury [J]. Turk Neurosurg, 2014, 24(3): 363-368.
[24] Zhang H, Adwanikar H, Werb Z, et al. Matrix metalloproteinases and neurotrauma: evolving roles in injury and reparative processes [J]. Neuroscientist, 2010, 16(2): 156-170.
[25] Suehiro E, Fujisawa H, Akimura T, et al. Increased matrix metalloproteinase-9 in blood in association with activation of interleukin-6 after traumatic brain injury: influence of hypothermic therapy [J]. J Neurotrauma, 2004, 21(12): 1706-1711.

Effect of Treadmill Exercise on Expression of Matrix Metalloproteinase-2 in Rats with Traumatic Brain Injury

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