磷脂酰肌醇3激酶、蛋白激酶B、哺乳动物雷帕霉素靶蛋白在帕金森病模型大鼠黑质区的表达

doi:10.3969/j.issn.1006-9771.2017.09.011

Abstract

Abstract: ObjectiveTo observe the expression of phosphatidylinositol 3-kinase (PI3K) / protein kinase B (Akt) / mammalian target of rapamycin (mTOR) signaling pathway in substantia nigra in rats with Parkinson's disease (PD). MethodsA total of 96 Sprague-Dawley rats were randomly divided into sham group, model group, PI3K inhibitor LY294002 group and mTOR inhibitor rapamycin group. Each group was divided into 4 days and 8 days subgroups after the model. PD model was established by injecting rotenone subcutaneously. The expression of PI3K, p-Akt and p-mTOR in substantia nigra was detected with immunohistochemistry and Western blotting. ResultsCompared with the sham group, the expression of PI3K, p-Akt and p-mTOR increased in the model group (P<0.05), and was more in 8 days subgroup than in 4 days subgroup (P<0.05). Compared with the model group, the expression of p-Akt and p-mTOR reduced in the LY294002 group (P<0.05), while the expression of PI3K varied little (P>0.05); the expression of p-mTOR decreased in the rapamycin group (P<0.05), while the expression of PI3K and p-Akt varied little (P>0.05). ConclusionPI3K/Akt/mTOR signaling pathway is over activated in substantia nigra in rats with Parkinson's disease, which may play an important role in occurrence and development of the disease.

Key words: Parkinson's disease, phosphatidylinositol 3-kinase, protein kinase B, mammalian target of rapamycin, substantia nigra, rats

CLC Number:

R742.5

HU Xin-yue, LIU Bin, XU Jia-li, ZHANG Jin-xia. Expression of Phosphatidylinositol 3-Kinase, Protein Kinase B and Mammalian Target of Rapamycin in Substantia Nigra in Rats with Parkinson's Disease[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(9): 1043-1050.

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Expression of Phosphatidylinositol 3-Kinase, Protein Kinase B and Mammalian Target of Rapamycin in Substantia Nigra in Rats with Parkinson's Disease

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