体外培养大鼠星形胶质细胞水通道蛋白4的表达

doi:10.3969/j.issn.1006-9771.2017.09.012

Abstract

Abstract: ObjectiveTo explore the expression of aquaporin 4 (AQP4) in primary and secondary cultured rat astrocytes. MethodsRat cortical astrocytes from a newborn (one day) Wistar rat were cultured. Astrocytes were identified with immunofluorescence staining of glial fibrilillary acidic protein (GFAP). The expression of AQP4 was determined with real-time quantitative polymerase chain reaction and immunofluorescence staining three, five, seven and nine days of primary culture, and nine days of secondary culture. ResultsThe purity of GFAP-positive cells was more than 95%. The expression of AQP4 mRNA was found three days of primary culture, remained unchanged five days of primary culture (P>0.05), and increased seven and nine days of primary culture (P<0.05). The expression of AQP4 mRNA was not different between nine days of primary culture and nine days of secondary culture (P>0.05). AQP4 immunofluorescence staining showed the same trend of AQP4 mRNA. ConclusionAQP4 may express since three days of primary culture in rat astrocytes in vitro, and increase slowly until nine days of primary culture.

Key words: aquaporin 4, astrocyte, cell culture, rats

CLC Number:

R338.2

XU Li-xin, DONG Li-ping, SHI Zhong-fang, YAN Xu, YANG Shao-hua, YUAN Fang. Expression of Aquaporin 4 in Astrocytes of Rats in Vitro[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(9): 1051-1055.

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Expression of Aquaporin 4 in Astrocytes of Rats in Vitro

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