电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马5-羟色胺1A受体表达的影响

doi:10.3969/j.issn.1006-9771.2017.10.005

Abstract

Abstract: Objective To explore the effect of electroacupuncture at Baihui (DU20) and Shenting (DU24) acupoints on learning and memory ability in rats with middle cerebral artery occlusion and reperfusion (MCAO/R), and the potential mechanisms.Methods A total of 90 healthy male Sprague-Dawley rats were randomly divided into sham group (n=18) and operation group (n=72). The MCAO/R model was established by suture method in the operation group. Finally, 54 qualified rats of the operation group were randomly divided into ischemia group (n=18), electroacupuncture group (n=18) and non-acupoint group (n=18). The electroacupuncture group received electroacupuncture at Baihui (DU20) and Shenting (DU24) for 14 days. The cerebral infarction volume was measured by magnetic resonance imaging (MRI). The learning and memory ability was tested by Morris water maze. The protein expression of 5-hydroxytryptamine 1A (5-HT_1A) receptor was detected by immunohistochemistry.Results There was no significant difference in the cerebral infarction volume among three groups before intervention (F=1.678, P>0.05). Compared with the ischemia group and the non-acupoint group, the cerebral infarction volume significantly reduced (P<0.001); the latency significantly shortened (P<0.001) and the times crossing the platform decreased (P<0.05); the expression of 5-HT_1A receptor decreased in the left hippocampus (P<0.05) in the electroacupuncture group after intervention. There was no significant difference in all the indices between the non-acupoint group and the ischemia group after intervention (P>0.05).Conclusion Electroacupuncture at Baihui (DU20) and Shenting (DU24) could effectively increase the learning and memory ability of MCAO/R rats, which might relate with inhibiting the expression of 5-HT_1A receptor in hippocampus.

Key words: cerebral ischemia-reperfusion, electroacupuncture, learning and memory, 5-hydroxytryptamine 1A receptor, rats

CLC Number:

R743.3

JIN Hao, LIU Wei-lin, LIN Bing-bing, LI Long, PENG Hong-wei, ZHANG Xiu-feng, SONG Chang-ming, HUANG Jia, TAO Jing, CHEN Li-dian. Effects of Electroacupuncture at Baihui and Shenting Acupoints on Learning and Memory Ability and Expression of 5-hydroxytryptamine 1A Receptor in Rats with Cerebral Ischemia-Reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(10): 1145-1151.

References

[1] 中华医学会神经病学分会神经康复学组,中华医学会神经病学分会脑血管病学组,卫生部脑卒中筛查与防治工程委员会办公室. 张通执笔. 中国脑卒中康复治疗指南(2011 完全版)[J]. 中国康复理论与实践, 2012, 18(4): 301-318.
[2] Sun H, Zou X, Liu L. Epidemiological factors of stroke: a survey of the current Status in China [J]. J Stroke, 2013, 15(2): 109-114.
[3] Park JH, Kim BJ, Bae HJ, et al. Impact of post-stroke cognitive impairment with no dementia on health-related quality of life [J]. J Stroke, 2013, 15(1): 49-56.
[4] Savva GM, Stephan BC. Epidemiological studies of the effect of stroke on incident dementia: a systematic review [J]. Stroke, 2010, 41(1): e41-e46.
[5] Li W, Huang R, Shetty RA, et al. Transient focal cerebral ischemia induces long-term cognitive function deficit in an experimental ischemic stroke model [J]. Neurobiol Dis, 2013, 59: 18-25.
[6] Dacosta-Aguayo R, Grana M, Fernandez-Andujar M, et al. Structural integrity of the contralesional hemisphere predicts cognitive impairment in ischemic stroke at three months [J]. PLoS One, 2014, 9(1): e86119.
[7] Yang S, Ye H, Huang J, et al. The synergistic effect of acupuncture and computer-based cognitive training on poststroke cognitive dysfunction：a study protocol for a randomized controlled trial of 2×2 factorial design [J]. BMC Complement Altern Med, 2014, 14: 290.
[8] 冯晓东,刘娇,陈立典. 电针对局灶性脑缺血大鼠学习记忆能力的影响及其机制[J]. 中国康复理论与实践, 2013, 19(3): 227-230.
[9] Berumen LC, Rodríguez A, Miledi R, et al. Serotonin receptors in hippocampus [J]. Scientific World Journal, 2012, 2012: 823493.
[10] Glikmann-Johnston Y, Saling MM, Chen J, et al. Hippocampal 5-HT 1A receptor binding is related to object-location memory in humans [J]. Brain Struct Funct, 2015, 220(1): 559-570.
[11] Ogren SO, Eriksson TM, Elvander-Tottie E, et al. The role of 5-HT(1A) receptors in learning and memory [J]. Behav Brain Res, 2008, 195(1): 54-77.
[12] 段晓晶. 针刺对脑卒中后抑郁大鼠单胺类递质及受体影响的实验研究[D]. 郑州:河南中医学院, 2013.
[13] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion and reperfusion without craniectomy in rats [J]. Stroke, 1989, 20(1): 84-91.
[14] 李忠仁. 实验针灸学[M]. 北京:中国中医药出版社, 2007: 327-329.
[15] Zhang YF, Yu JC, Zhang XZ, et al. Effect of acupuncture intervention on hippocampal neuron loss and astrocytosis in SAMP 8 mice [J]. Zhen Ci Yan Jiu, 2013, 38(5): 358-364.
[16] Feng XD, Yang SL, Liu J, et al. Electroacupuncture ameliorates cognitive impairment through inhibition of NF-κB-mediated neuronal cell apoptosis in cerebral ischemia reperfusion injured rats [J]. Mol Med Rep, 2013, 7(5): 1516-1522.
[17] Liu F, Li ZM, Jiang YJ, et al. A meta-analysis of acupuncture use in the treatment of cognitive impairment after stroke [J]. J Altern Complement Med, 2014, 20(7): 535-544.
[18] 吴羽楠,张蕴,林如辉,等.电针对脑缺血再灌注大鼠学习记忆能力及RhoA蛋白表达的影响[J]. 中国康复理论与实践, 2015, 21(1): 17-21.
[19] Bannerman DM, Sprengel R, Sanderson DJ, et al. Hippocampal synaptic plasticity, spatial memory and anxiety [J]. Nat Rev Neurosci, 2014, 15(3): 181-192.
[20] Witte AV, Kerti L, Margulies DS, et al. Effects of resveratrol on memory performance, hippocampal functional connectivity, and glucose metabolism in healthy older adults [J]. J Neurosci, 2014, 34(23): 7862-7870.
[21] Horner AJ, Doeller CF. Plasticity of hippocampal memories in humans [J]. Curr Opin Neurobiol, 2017, 43: 102-109.
[22] 郭瑞,那杰,白旭,等. 5-HT 1A 受体参与学习记忆的分子机制研究进展[J]. 现代生物医学进展, 2014, 14(12): 2390-2393.
[23] Glikmann-Johnston Y, Saling MM, Reutens DC, et al. Hippocampal 5-HT 1A receptor and spatial learning and memory [J]. Front Pharmacol, 2015, 6: 289.
[24] Park J, Chávez AE, Mineur YS, et al. CaMKII phosphorylation of TARPγ-8 is a mediator of LTP and learning and memory [J]. Neuron, 2016, 92(1): 75-83.
[25] Le Franois B, Soo J, Millar AM, et al. Chronic stress antidepressant treatment alter mild 5-HT 1A and receptor expression by DNA methylation of a modifying conserved Sp4 site [J]. Neurobiol Dis, 2015, 82: 332-341.
[26] Berends AC, Luiten PG, Nyakas C. A review of the neuroprotective properties of the 5-HT 1A receptor agonist repinotan HCl (BAYx3702) in ischemic stroke [J]. CNS Drug Rev, 2005, 11(4): 379-402.
[27] Buhot M, Martin S, Segu L. Role of serotonin in memory impairment [J]. Ann Med, 2000, 32(3): 210-221.
[28] Lee CH, Ahn JH, Won MH. New expression of 5-HT 1A receptor in astrocytes in the gerbil hippocampal CA1 region following transient global cerebral ischemia [J]. Neurol Sci, 2015, 36(3): 383-389.
[29] 林玲,刘国良,孙缦利. 海马CA1区5-HT 1A 受体调控PTSD大鼠空间记忆的作用[J]. 中国病理生理杂志, 2017, 33(1): 98-103.

Effects of Electroacupuncture at Baihui and Shenting Acupoints on Learning and Memory Ability and Expression of 5-hydroxytryptamine 1A Receptor in Rats with Cerebral Ischemia-Reperfusion

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	CHEN Yiting, WANG Qian, CUI Shenhong, LI Yingcai, ZHANG Siyu, WEI Yanxu, REN Hui, LENG Jun, CHEN Bin. Effect of bilateral sequential repetitive transcranial magnetic stimulation on motor function of upper limbs in stroke patients [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(8): 926-932.
[2]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[3]	KANG Xiaoyu, LIU Lixu, WANG Wenzhu, WANG Yunlei. Effects of pramipexole combined with levodopa on cognitive and mitochondrial function of rats after global cerebral ischemia-reperfusion injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 533-540.
[4]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[5]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[6]	ZHANG Li,WU Shanhong,ZHAO Lele,WANG Yan. Role of Netrin-1 and Slit2 in regulating the imbalance of Rho GTPases after Jiaji electroacupuncture combined with nerve mobilization for sciatic nerve injury in rabbits [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(8): 914-926.
[7]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.
[8]	ZHOU Xiaojue,FENG Jing,PANG Rizhao,LIU Jie,ZHANG Anren. Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 544-551.
[9]	SONG Shaofei,HOU Yuanyuan,WANG Yunlei,ZHANG Tong. Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 552-558.
[10]	LI Tong,FANG Zhipeng,SHAO Yuping,WANG Ping. Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1270-1277.
[11]	DING Yanyi,ZHANG Shenghang,LIU Yulu,YU Yan,YANG Minguang,LIANG Shengxiang,LIU Weilin,TAO Jing. Effect of electroacupuncture on regional homogeneity of brain function in rats with vascular cognitive impairment [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(1): 55-61.
[12]	Jing-yi WANG,Jie YIN,Jian-cheng LIU,Ri-zhao PANG,Wen-chun WANG. Effect of Iridoid-rich Fraction from Valeriana Jatamansi Jones on Neuron Pyroptosis in Rats with Acute Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 653-660.
[13]	Wen-mei ZHOU,Tao TAO,Shuang WU,Ting-long WANG,Zheng-yi YANG,Ying ZHANG. Effects of Enriched Environment on Neurological Function and Glucose Metabolism in Ischemic Penumbra in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(5): 522-529.
[14]	Qiong-fen WANG,Feng-bo WANG,Ke WANG,Yong-qiang ZHONG,Jiao-jiao WANG. Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(3): 302-309.
[15]	Xiang-zhe LI,Jie DING,Qing-hua WANG,Chuan-ming DONG,Tong WANG,Qin-feng WU. Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase (GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 131-136.