电针百会、神庭穴对缺血再灌注损伤大鼠学习记忆能力及海马CA1区嘌呤受体P2X7表达的影响

doi:10.3969/j.issn.1006-9771.2018.01.008

Abstract

Abstract: Objective To observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning and memory in rats after cerebral ischmeia-reperfusion and the possible mechanism.Methods A total of 42 male Sprague-Dawley rats were randomly divided into sham group (n=12) and operation group (n=30). The left middle cerebral arteries of the operation group were occluded with the modified Longa's method for 90 minutes and reperfused, and 24 qualified rats were randomly divided into model group (n=12) and electroacupuncture group (n=12), and the latter accepted electroacupuncture at Baihui and Shenting for seven days. They were assessed with Longa's score two hours after modeling, and one, three, seven days after intervention. They were tested with Barnes maze since three days after intervention, once a day for five days. The expression of purinoceptor P2X7 in CA1 of the hippocampus were detected with immunofluorescence seven days after intervention, while the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in CA1 were detected with enzyme-linked immunosorbent assay.Results The Longa's score was improved in the electroacupuncture group compared with that in the model group seven days after intervention (P<0.05); while the escape latency and the times entering the wrong hole increased in the model group compared with that in the sham group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.001). The expression of P2X7, IL-1β and TNF-α increased in the model group compared with the sham operation group (P<0.001), and decreased in the electroacupuncture group compared with that in the model group (P<0.05).Conclusion Electroacupuncture at Baihui and Shenting can improve the learning and memory in rats after cerebral ischemia-reperfusion, which may associate with inhibition of P2X7 to alleviate inflammation in hippocampus.

Key words: cerebral ischemia-reperfusion, electroacupuncture, purinoceptor, P2X7, hippocampus, learning and memory, inflammation, rats

CLC Number:

R743.3

HUANG Jia, SONG Chang-ming, YANG Min-guang, LI Jian-hong, HUANG Sheng, LIU Wei-lin, TAO Jing, CHEN Li-dian. Effects of Electroacupuncture at Baihui and Shenting on Learning and Memory and Expression of Purinoceptor P2X7 in Hippocampal CA1 after Cerebral Ischmeia-reperfusion in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 43-48.

References

[1] Mozaffarian D, Benjamin EJ, Go AS, et al. Executive Summary: Heart Disease and Stroke Statistics-2016 Update: a report from the American Heart Association [J]. Circulation, 2016, 133(4): 447-454.
[2] Murphy SL, Xu J, Kochanek KD, et al. Deaths: Final Data for 2013 [J]. Natl Vital Stat Rep, 2016, 64(2): 1-119.
[3] Agnès J, Christine B, Olivier R, et al. Post-stroke cognitive impairment: high prevalence and determining factors in a cohort of mild stroke [J]. J Alzheimers Dis, 2014, 40(4): 1029-1038.
[4] Taylor RA, Sansing LH. Microglial responses after ischemic stroke and intracerebral hemorrhage [J]. Clin Dev Immunol, 2013, 2013: 746068.
[5] Feng X, Yang S, Liu J, et al. Electro-acupuncture ameliorates cognitive impairment through inhibition of NF-κB-mediated neuronal cell apoptosis in cerebral ischemia-reperfusion injured rats [J]. Mol Med Rep, 2013, 7(5): 1516-1522.
[6] 宋长明,黄佳,林冰冰,等. 电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响[J]. 中国康复理论与实践, 2017, 23(7): 750-755.
[7] 林志诚,杨珊莉,薛偕华,等. 针刺百会穴改善脑卒中患者记忆力的中枢机制[J]. 中国康复理论与实践, 2015, 20(2): 184-188.
[8] Jiang C, Yang S, Tao J, et al. Clinical Efficacy of acupuncture treatment in combination with RehaCom Cognitive Training for improving cognitive function in stroke: a 2×2 factorial design randomized controlled trial [J]. J Am Med Dir Assoc, 2016, 17(12): 1114-1122.
[9] Tort ABL, Komorowski RW, Manns JR, et al. Theta-gamma coupling increases during the learning of item-context associations [J]. Proc Natl Acad Sci USA, 2009, 106(49): 20942-20947.
[10] Nikonenko AG, Radenovic L, Andjus PR, et al. Structural features of ischemic damage in the hippocampus [J]. Anat Rec, 2009, 292(12): 1914-1921.
[11] Kerchner GA, Hess CP, Hammondrosenbluth KE, et al. Hippocampal CA1 apical neuropil atrophy in mild Alzheimer disease visualized with 7-T MRI (Podcast) [J]. Neurology, 2010, 75(15): 1381-1387.
[12] Kerchner GA, Deutsch GK, Zeineh M, et al. Hippocampal CA1 apical neuropil atrophy and memory performance in Alzheimer's disease [J]. Neuroimage, 2012, 63(1): 194-202.
[13] Pankratov YV, Lalo UV, Krishtal OA. Role for P2X receptors in long-term potentiation [J]. J Neurosci, 2002, 22(19): 8363-8369.
[14] Chin Y, Kishi M, Sekino M, et al. Involvement of glial P2Y1 receptors in cognitive deficit after focal cerebral stroke in a rodent model [J]. J Neuroinflammation, 2013, 10(1): 95.
[15] Illes P, Verkhratsky A. Purinergic neurone-glia signalling in cognitive-related pathologies [J]. Neuropharmacology, 2016, 104: 62-75.
[16] Amantea D, Micieli G, Tassorelli C, et al. Rational modulation of the innate immune system for neuroprotection in ischemic stroke [J]. Front Neurosci, 2015, 9(12): 2366-2378.
[17] Burnstock G. P2X ion channel receptors and inflammation [J]. Purinergic Signal, 2016, 12(1): 59-67.
[18] Deng Y, Guo XL, Yuan X, et al. P2X7 receptor antagonism attenuates the intermittent hypoxia-induced spatial deficits in a murine model of sleep apnea via inhibiting neuroinflammation and oxidative stress [J]. Chin Med J (Engl), 2015, 128(16): 2168-2175.
[19] Chu K, Yin B, Wang J, et al. Inhibition of P2X7 receptor ameliorates transient global cerebral ischemia/reperfusion injury via modulating inflammatory responses in the rat hippocampus [J]. J Neuroinflammation, 2012, 9(1): 150-159.
[20] Joana A, Alberto PS, Miroslav G, et al. P2X7 receptor blockade prevents ATP excitotoxicity in neurons and reduces brain damage after ischemia [J]. Neurobiol Dis, 2012, 45(3): 954-961.
[21] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J]. Stroke, 1989, 20(1): 84-91.
[22] 李忠仁. 实验针灸学[M]. 北京:中国中医药出版社, 2003.
[23] 李梦瑶,李向荣,高琼珏,等. 电针对局灶性脑缺血再灌注大鼠血中EPCs含量及其神经功能缺损程度的影响[J]. 中医药通报, 2017, 16(3): 39-42.
[24] 皮敏,陈鹏典,杨卓欣,等. 电针督脉联合人脐血间充质干细胞移植对脑缺血大鼠神经功能缺损及细胞凋亡的影响[J]. 时珍国医国药, 2014, 25(2): 492-495.
[25] 梁超,姜涛,王静芝,等. 不同变频组合电针预处理对急性脑缺血大鼠神经功能和脑皮质促红细胞生成素的影响[J]. 中国中医基础医学杂志, 2017, 23(5): 686-688.
[26] 蔡丽,刘毅,陆小青. 针刺对卒中后抑郁大鼠空间学习记忆功能及海马CA3区脑源性神经营养因子的影响[J]. 上海针灸杂志, 2016, 35(4): 462-465.
[27] 陈彬,张志涛,骆健明,等. 针刺治疗对缺血缺氧性脑病模型鼠学习与记忆能力的影响[J]. 中国医药, 2014, 9(8): 1159-1163.
[28] Burnstock G. Purinergic signaling in acupuncture [J]. Science, 2014, 346(6216): S23-S25.
[29] Weng ZJ, Wu LY, Zhou CL, et al. Effect of electro-acupuncture on P2X3 receptor regulation in the peripheral and central nervous systems of rats with visceral pain caused by irritable bowel syndrome [J]. Purinergic Signal, 2015, 11(3): 321-329.
[30] Guo X, Chen J, Yuan L, et al. Electro-acupuncture at He-Mu points reduces P2X4 receptor expression in visceral hypersensitivity [J]. Neural Regen Res, 2013, 8(22): 2069-2077.
[31] Liu W, Wang X, Zheng Y, et al. Electro-acupuncture inhibits inflammatory injury by targeting the miR-9-mediated NF-κB signaling pathway following ischemic stroke [J]. Mol Med Rep, 2016, 13(2): 1618-1626.
[32] Lan L, Tao J, Chen A, et al. Electro-acupuncture exerts anti-inflammatory effects in cerebral ischemia-reperfusion injured rats via suppression of the TLR4/NF-κB pathway [J]. Int J Mol Med, 2013, 31(1): 75-80.
[33] Chen SH, Sun H, Xu H, et al. [Effects of acupuncture of "Baihui"(GV 20) and "Zusanli"(ST 36) on expression of cerebral IL-1beta and TNF-alpha proteins in cerebral ischemia reperfusion injury rats] [J]. [in Chinese]. Zhen Ci Yan Jiu, 2012,37(6): 470-475.
[34] Cheng CY, Lin JG, Tang NY, et al. Electro-acupuncture-like stimulation at the Baihui (GV20) and Dazhui (GV14) acupoints protects rats against subacute-phase cerebral ischemia-reperfusion injuries by reducing S100B-mediated neurotoxicity [J]. PLoS One, 2014, 9(3): e91426.

Effects of Electroacupuncture at Baihui and Shenting on Learning and Memory and Expression of Purinoceptor P2X7 in Hippocampal CA1 after Cerebral Ischmeia-reperfusion in Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	CHEN Yiting, WANG Qian, CUI Shenhong, LI Yingcai, ZHANG Siyu, WEI Yanxu, REN Hui, LENG Jun, CHEN Bin. Effect of bilateral sequential repetitive transcranial magnetic stimulation on motor function of upper limbs in stroke patients [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(8): 926-932.
[2]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[3]	KANG Xiaoyu, LIU Lixu, WANG Wenzhu, WANG Yunlei. Effects of pramipexole combined with levodopa on cognitive and mitochondrial function of rats after global cerebral ischemia-reperfusion injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 533-540.
[4]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[5]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[6]	ZHANG Li,WU Shanhong,ZHAO Lele,WANG Yan. Role of Netrin-1 and Slit2 in regulating the imbalance of Rho GTPases after Jiaji electroacupuncture combined with nerve mobilization for sciatic nerve injury in rabbits [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(8): 914-926.
[7]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.
[8]	ZHOU Xiaojue,FENG Jing,PANG Rizhao,LIU Jie,ZHANG Anren. Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 544-551.
[9]	SONG Shaofei,HOU Yuanyuan,WANG Yunlei,ZHANG Tong. Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 552-558.
[10]	LI Tong,FANG Zhipeng,SHAO Yuping,WANG Ping. Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1270-1277.
[11]	LAN Wanting,ZHONG Jiugen,SHEN Yingying,GONG Jiaheng,ZOU Zhi,HOU Xiaohui. Mechanism of exercise reducing neuroinflammation in autism spectrum disorder: a visualized analysis [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(10): 1190-1197.
[12]	DING Yanyi,ZHANG Shenghang,LIU Yulu,YU Yan,YANG Minguang,LIANG Shengxiang,LIU Weilin,TAO Jing. Effect of electroacupuncture on regional homogeneity of brain function in rats with vascular cognitive impairment [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(1): 55-61.
[13]	Jing-yi WANG,Jie YIN,Jian-cheng LIU,Ri-zhao PANG,Wen-chun WANG. Effect of Iridoid-rich Fraction from Valeriana Jatamansi Jones on Neuron Pyroptosis in Rats with Acute Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 653-660.
[14]	Wen-mei ZHOU,Tao TAO,Shuang WU,Ting-long WANG,Zheng-yi YANG,Ying ZHANG. Effects of Enriched Environment on Neurological Function and Glucose Metabolism in Ischemic Penumbra in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(5): 522-529.
[15]	Qiong-fen WANG,Feng-bo WANG,Ke WANG,Yong-qiang ZHONG,Jiao-jiao WANG. Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(3): 302-309.