电针预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及凋亡相关蛋白表达的影响

doi:10.3969/j.issn.1006-9771.2018.01.010

Abstract

Abstract: Objective To investigate the effect of electroacupuncture (EA) preconditioning on apoptosis after cerebral ischemia-reperfusion (I/R).Methods A total of 72 male Sprague-Dawley rats were randomly divided into sham group (n=24), model group (n=24) and EA group (n=24). The rats in latter two groups were occluded the right middle cerebral arteries for two hours and reperfused. EA group was treated with EA at Baihui (GV20) for two weeks before modeling. They were assessed with modified Neurological Severity Scores (mNSS) 24 hours after modeling. Then, the cerebral infarct volume was measured with TTC staining, the apoptosis was detected with TUENL assay, and the expression of p53, Bax and Bcl-2 proteins in ischemic penumbra was detected with Western blotting.Results Compared with the model group, the score of mNSS, cerebral infarct volume and the number of TUNEL-positive cells all significantly decreased (P<0.05) in EA group; while the expression of p53 and Bax proteins decreased (P<0.05), Bcl-2 increased (P<0.05), and Bax/Bcl-2 decreased (P<0.05).Conclusion EA preconditioning can induce tolerance to cerebral I/R injury, which might associate with the inhibition of p53 protein and down-regulation of the Bax/Bcl-2 ratio in ischemic penumbra, to inhibit cerebral cell apoptosis.

Key words: cerebral ischemia-reperfusion, electroacupuncture, preconditioning, apoptosis, p53, Bax, Bcl-2, rats

CLC Number:

R743.3

YE Tao, ZHU Lu-wen, TANG Qiang, LI Hong-yu, WU Xiao-jun, CHEN Chen, JIANG Yun-fei, LI Jia-shuai. Effects of Electroacupuncture Preconditioning on Apoptosis and Expression of Apoptosis-related Proteins in Rats after Cerebral Ischemia-reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 54-59.

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Effects of Electroacupuncture Preconditioning on Apoptosis and Expression of Apoptosis-related Proteins in Rats after Cerebral Ischemia-reperfusion

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