沉默白细胞介素-1β基因对脊髓钝挫伤大鼠波形蛋白的影响

doi:10.3969/j.issn.1006-9771.2018.01.012

Abstract

Abstract: Objective To observe the expression of vimentin (Vim) after silence of interleukin-1β (IL-1β) in rats with spinal cord contusion (SCC).Methods The model of SCC was established in 30 Sprague-Dawley rats with Allen's method. The rats were randomized into vector group (n=15) and silence group (n=15), which were injected blank lentivirus vector and vector of IL-1β siRNA, respectively; and divided in three, seven and 28 days subgroups. The relationship between IL-1β and Vim was predicted with GeneMANIA bioinformatics. The expression of Vim protein and mRNA in spinal cord was detected with immunohistochemistry and real-time quantitative polymerase chain reaction.Results GeneMANIA bioinformatic analysis indicated that there was some direct and indirect relationship between IL-1β and Vim. The Vim protein and mRNA expressed in the spinal cord, and was less in the silence group than in the vector group (t>2.875, P<0.05). Conclusion Silence of IL-1β can inhibit the expression of Vim in SCC rats, which may promote the recovery of spinal cord function.

Key words: spinal cord contusion, interleukin-1β, vimentin, siRNA, rats

CLC Number:

R651.2

HU Xi, NIU Ying-jie, HUANG Yuan, LI Ying-jie, ZENG Xi, XIANG Yang, ZHANG Xiao, LIANG Nan. Effect of Interleukin-1β Silence on Expression of Vimentin in Rats with Spinal Cord Contusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 66-70.

References

[1] Toyooka T, Nawashiro H, Shinomiya N, et al. Down-regulation of glial fibrillary acidic protein and vimentin by RNA interference improves acute urinary dysfunction associated with spinal cord injury in rats [J]. Injury, 2011,28(4): 607-618.
[2] Bradbury EJ, Carter LM. Manipulating the glial scar: chondroitinase ABC as a therapy for spinal cord injury [J]. Brain Res Bull, 2011, 84(4): 306-316.
[3] Cemil B, Topuz K, Demircan MN, et al. Curcumin improves early functional results after experimental spinal cord injury [J]. Acta Neurochir (Wien), 2010, 152(9): 1583-1590.
[4] de Rivero JP, Lotocki G, Marcillo AE, et al. A molecular platform in neurons regulates inflammation after spinal cord injury [J]. J Neurosci, 2008, 28(13): 3404-3414.
[5] Cheng Z, Zhu W, Cao K, et al. Anti-inflammatory mechanism of neural stem cell transplantation in spinal cord injury [J]. Int J Mol Sci, 2016, 17(9): E1380.
[6] Cong L, Chen W. Neuroprotective effect of ginsenoside Rd in spinal cord injury rats [J]. Basic Clin Pharmacol Toxicol, 2016, 119(2): 193-201.
[7] Zhao J, Wang L, Li Y. Electroacupuncture alleviates the inflammatory response via effects on M1 and M2 macrophages after spinal cord injury [J]. Acupunct Med, 2017, 35(3): 224-230.
[8] Biglari B, Swing T, Child C, et al. A pilot study on temporal changes in IL-1beta and TNF-alpha serum levels after spinal cord injury: the serum level of TNF-alpha in acute SCI patients as a possible marker for neurological remission [J]. Spinal Cord, 2015, 53(7): 510-514.
[9] Endo T, Ajiki T, Inoue H, et al. Early exercise in spinal cord injured rats induces allodynia through TrkB signaling [J]. Biochem Biophys Res Commun, 2009, 381(3): 339-344.
[10] Sun D, Lye-Barthel M, Masland RH, et al. Structural remodeling of fibrous astrocytes after axonal injury [J]. J Neurosci, 2010, 30: 14008-14019.
[11] Ekmark-Lewén S, Flygt J, Kiwanuka O, et al. Traumatic axonal injury in the mouse is accompanied by a dynamic inflammatory response, astroglial reactivity and complex behavioral changes [J]. J Neuroinflam, 2013, 10: 44.
[12] Lee KW, Zhao X, Im JY, et al. Apoptosis signal-regulating kinase 1 mediates MPTP toxicity and regulates glial activation [J]. PLoS One, 2012, 7(1): e29935.
[13] Wang X, Budel S, Baughman K, et al. Ibuprofen enhances recovery from spinal cord injury by limiting tissue loss and stimulating axonal growth [J]. J Neurotrauma, 2009, 26(4): 81-95.
[14] Lin B, Xu Y, Zhang B, et al. MEK inhibition reduces glial scar formation and promotes the recovery of sensorimotor function in rats following spinal cord injury [J]. Exp Ther Med, 2014, 7(1): 66-72.
[15] Ritz MF, Hausmann ON. Effect of 17beta-estradiol on functional outcome, release of cytokines, astrocyte reactivity and inflammatory spreading after spinal cord injury in male rats [J]. Brain Res, 2008, 1203: 177-188.
[16] Heimfarth L, Loureiro SO, Dutra MF, et al. In vivo treatment with diphenyl ditelluride induces neurodegeneration in striatum of young rats: Implications of MAPK and Akt pathways [J]. Toxicol Appl Pharmacol, 2012, 264: 143-152.
[17] Sutoh Yoneyama M, Hatakeyama S, Habuchi T, et al. Vimentin intermediate filament and plectin provide a scaffold for invadopodia, facilitating cancer cell invasion and extravasation for metastasis [J]. Eur J Cell Biol, 2014, 93(4): 157-169.
[18] Desclaux M, Perrin FE, Do-Thi A, et al. Lentiviral-mediated silencing of glial fibrillary acidic protein and vimentin promotes anatomical plasticity and functional recovery after spinal cord injury [J]. Neurosci Res, 2015, 93(1): 43-55.
[19] Li TZ, Yan Y, Liu Q, et al. Effect of suppressing apoptosis signal regulating kinase 1 on GFAP and vimentin expression and hindlimb mobility in rats after spinal cord injury [J]. Nan Fang Yi Ke Da Xue Xue Bao, 2015, 35(6): 795-800.
[20] Xia Y, Zhao T, Li J, et al. Antisense vimentin cDNA combined with chondroitinase ABC reduces glial scar and cystic cavity formation following spinal cord injury in rats [J]. Biochem Biophys Res Commun, 2008, 377(2): 562-566.
[21] Low K, Blesch A, Herrmann J, et al. A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury [J]. Gene Ther, 2010, 17(5): 577-591.
[22] Ismailoğlu Ö, Oral B, Sütcü R, et al. Neuroprotective effects of raloxifene on experimental spinal cord injury in rats [J]. Am J Med Sci, 2013, 345(1): 39-44.
[23] 宋兴华,曹力,艾尔肯,等. 大鼠慢性颈髓损伤后颈髓中IL-1β的表达及其与神经细胞凋亡的关系[J]. 中国脊柱脊髓杂志, 2007, 17(3): 222-225.
[24] Adams RA, Bauer J, Fliek MJ, et al. The fibrin-derived γ377¬395 peptide inhibits mieroglia activation and suppresses relapsing paralysis in central nervous system autoimmune disease [J]. J Exp Med, 2007, 204(7): 571-582.
[25] Hernandez-Rodriguez J, Segarra M, Vilardell C, et al. Tissue production of pro-inflammatory cytokines (IL-1beta, TNF-alpha and IL-6) correlates with the intensity of the systemic inflammatory response and with corticosteroid requirements in giant-cell arteritis [J]. Rheumatology (Oxford), 2004, 43(3): 294-301.
[26] Nesic O, Xu GY, Mcadoo D, et al. IL-1β receptor antagonist prevents apoptosis and caspase-3 activation after spinal cord injury [J]. J Neurotrauma, 2001, 18(9): 947-956.
[27] Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, et al. Spinal cord injury induces a long-lasting upregulation of interleukin-1β in astrocytes around the central canal [J]. Neuroscience, 2015, 284: 283-289.

Effect of Interleukin-1β Silence on Expression of Vimentin in Rats with Spinal Cord Contusion

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[2]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[3]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[4]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.
[5]	ZHOU Xiaojue,FENG Jing,PANG Rizhao,LIU Jie,ZHANG Anren. Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 544-551.
[6]	SONG Shaofei,HOU Yuanyuan,WANG Yunlei,ZHANG Tong. Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 552-558.
[7]	LI Tong,FANG Zhipeng,SHAO Yuping,WANG Ping. Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1270-1277.
[8]	Jing-yi WANG,Jie YIN,Jian-cheng LIU,Ri-zhao PANG,Wen-chun WANG. Effect of Iridoid-rich Fraction from Valeriana Jatamansi Jones on Neuron Pyroptosis in Rats with Acute Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 653-660.
[9]	Wen-mei ZHOU,Tao TAO,Shuang WU,Ting-long WANG,Zheng-yi YANG,Ying ZHANG. Effects of Enriched Environment on Neurological Function and Glucose Metabolism in Ischemic Penumbra in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(5): 522-529.
[10]	Qiong-fen WANG,Feng-bo WANG,Ke WANG,Yong-qiang ZHONG,Jiao-jiao WANG. Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(3): 302-309.
[11]	Xiang-zhe LI,Jie DING,Qing-hua WANG,Chuan-ming DONG,Tong WANG,Qin-feng WU. Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase (GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 131-136.
[12]	Kun AI,Ming XU,Qiong LIU,Shi-feng DENG,Ji-sheng LIU,Fang QI,Xi-qin YI,Qi-rui QU,Hong ZHANG. Effect of Electroacupuncture on Content of Cyclic Adenosine Monophosphate and Protein Kinase A, and Phosphorylation of Myosin Light Chain Kinase in Detrusor of Rats with Detrusor Hyperreflex after Suprasacral Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 137-144.
[13]	LING Meng-yu,YANG Yi-zhuo,LIU Shuai,YE Chao-qun. Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(11): 1272-1281.
[14]	PENG Zhi-feng, ZHANG Yi-ping, ZHANG Ji-hong. Effect of Early Exercise Combined with Pentylenetetrazol on Neurologic Function after Cerebral Ischemia-reperfusion in Rats and Its Mechanism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(1): 27-30.
[15]	CHEN Xiao-ping,WANG Ming-yang,MA Deng-lei,GONG Shi-li,ZHANG Li,LI Ya-li,LI Lin,HU Chao-ying,ZHANG Lan. Effects of Analgecine on Apoptosis and Neuroinflammation in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(9): 1038-1044.