隔日限食疗法对脊髓损伤大鼠的保护作用及其机制

doi:10.3969/j.issn.1006-9771.2018.02.007

Abstract

Abstract: Objective To observe the effect of every-other-day fasting (EODF) on pathology and functional recovery of rats with spinal cord clip-compression injury, and to explore the related mechanism.Methods A total of 36 Sprague-Dawley rats were randomly assigned to groups A (sham operation), B (sham operation and EODF), C (spinal cord injury) and D (spinal cord injury and EODF) with nine rats in each group. The spinal cord injury rat model in T₁₀ was established by using medical aneurysm clip in latter two groups. The motor function was assessed by Basso-Beattie-Bresnahan (BBB) score one day before and one day, two, four, six, eight, ten, twelve weeks after operation; and toluidine blue staining was performed for pathological observation at twelve weeks after operation. Another 180 Sprague-Dawley rats were randomly assigned in same way. The level of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were detected with ELISA six hours, twelve hours, one day, three days and seven days after operation.Results The BBB score reached lowest on the first day in groups C and D (P<0.05), and gradually increased with time, and were higher in group D than in group C eight weeks, ten weeks and twelve weeks after operation (P<0.05). The pathology in spinal cord was less in group D than in group C. Compared with group A, the level of serum TNF-α increased twelve hours after operation (P<0.05), peaked one day after operation, and returned back seven days after operation; the level of serum IL-10 increased at each time point after operation (P<0.05). Compared with group C, the level of serum TNF-α was lower in group D one day after operation (P<0.05); the level of serum IL-10 was not significantly different at each time point after operation (P>0.05).Conclusion Long-term EODF can promote the hind limb motor recovery in rats with spinal cord clip-compression injury, and alleviate pathological damage. EODF might inhibit acute inflammatory reaction at acute stage of spinal cord injury, which may be correlated with its neuroprotective effect.

Key words: spinal cord injury, every-other-day fasting, motor function, tissue structure, inflammatory response, rats

CLC Number:

R651.2

HE Yu, ZHANG An-ren, SUN Nian-yi, WANG Wen-chun, WANG Zhi-qiang, TIAN Li-jun, ZOU Wen-chen, ZHANG Zhi-qiang. Protective Effect and Mechanism of Every-other-day Fasting on Rats with Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(2): 153-159.

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Protective Effect and Mechanism of Every-other-day Fasting on Rats with Spinal Cord Injury

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