运动预处理对脑缺血再灌注大鼠线粒体ATP敏感性钾通道蛋白及细胞凋亡的影响

doi:10.3969/j.issn.1006-9771.2018.05.001

Abstract

Abstract: Objective To explore the effect and mechanism of exercise preconditioning on neurological deficits in rats after cerebral ischemia-reperfusion. Methods Thirty-six healthy Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12) and exercise preconditioning group (n=12). The latter two groups were occluded middle cerebral artery for 120 minutes and reperfused with modified suture method. The rats were evaluated with Longa's score two, twelve and 24 hours after reperfusion. The expression of mitochondrial ATP-sensitive potassium (mitoK_ATP) channel proteins inwardly rectifying potassium channel (Kir6.2) and sulphonylurea receptor 1 (SUR1) were detected with Western blotting and the cerebral cell apoptosis was detected with TUENL assay 24 hours after reperfusion. Results Compared with the model group, the Longa's score decreased in the exercise preconditioning group 24 hours after reperfusion (P<0.05), while the expression of Kir6.2 and SUR1 decreased (P<0.05), and TUNEL-positive cells decreased (P<0.05).Conclusion Exercise preconditioning may improve neurological function after cerebral ischemia-reperfusion, which may associate with inhibiting the expression of mitoK_ATP channel proteins and cell apoptosis.

Key words: cerebral ischemia-reperfusion, exercise, preconditioning, mitochondrial ATP-sensitive potassium channels, cell apoptosis, rats

CLC Number:

R743.3

LI Hong-yu, TANG Qiang, ZHU Lu-wen, WANG Xue, ZHENG Ting-ting, CHEN Yu-hong, ZHAO Yi-dian, YIN Xia. Effect of Exercise Preconditioning on Cell Apoptosis and Expression of Mitochondrial ATP-sensitive Potassium Channels Proteins in Rats after Cerebral Ischemia-reperfusion[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(5): 497-501.

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Effect of Exercise Preconditioning on Cell Apoptosis and Expression of Mitochondrial ATP-sensitive Potassium Channels Proteins in Rats after Cerebral Ischemia-reperfusion

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