超短波促进巨噬细胞表型转化并抑制大鼠脊髓损伤后炎症反应的作用

doi:10.3969/j.issn.1006-9771.2018.06.003

Abstract

Abstract: Objective To explore the effect of ultrashort wave on macrophage polarization and inflammation response after spinal cord injury in rats.Methods A total of 96 Sprague-Dawley rats were randomly divided into sham group, model group and ultrashort wave group, with 32 rats in each group. The spinal cord injury model was established by modified Allen's method in the model group and the ultrashort wave group. The ultrashort wave group received ultrashort wave since the first day after modeling. BBB score was applied to evaluate the locomotion recovery. HE staining was used to assess the injury area, while immunochemistry, real-time PCR and Western blotting were applied to measure the mRNA and protein expression of inducible nitric oxide synthase (iNOS), arginase-1 (Arg-1) and tumor necrosis factor-α (TNF-α).Results Compared with the model group, the BBB score increased (P<0.05), the expression of iNOS and TNF-α decreased, and the expression of Arg-1 increased (P<0.05). Conclusion Ultrashort wave therapy may promote macrophage polarization from M1 to M2, inhibit inflammatory response and promote recovery after spinal cord injury in rats.

Key words: spinal cord injury, ultrashort wave, macrophage polarization, tumor necrosis factor-α, repair, rats

CLC Number:

R651.2

GAO Lin, FENG Zhi-ping, DAI Chen-fei, ZHANG Zhi-qiang, ZHANG Li-xin. Effect of Ultrashort Wave on Macrophage Polarization and Inflammatory Response after Spinal Cord Injury in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(6): 634-640.

References

[1] Majdan M, Plancikova D, Nemcovska E, et al.Mortality due to traumatic spinal cord injuries in Europe: a cross-sectional and pooled analysis of population-wide data from 22 countries[J]. Scand J Trauma Resusc Emerg Med, 2017, 25(1): 64.
[2] Ahuja CS, Wilson JR, Nori S, et al.Traumatic spinal cord injury[J]. Nat Rev Dis Primers, 2017, 3: 17018.
[3] Shultz RB, Zhong Y.Minocycline targets multiple secondary injury mechanisms in traumatic spinal cord injury[J]. Neural Regen Res, 2017, 12(5): 702-713.
[4] Bermudez S, Khayrullina G, Zhao Y, et al.NADPH oxidase isoform expression is temporally regulated and may contribute to microglial/macrophage polarization after spinal cord injury[J]. Mol Cell Neurosci, 2016, 77: 53-64.
[5] Kong X, Gao J.Macrophage polarization: a key event in the secondary phase of acute spinal cord injury[J]. J Cell Mol Med, 2017, 21(5): 941-954.
[6] Geng CK, Cao HH, Ying X, et al.The effects of hyperbaric oxygen on macrophage polarization after rat spinal cord injury[J]. Brain Res, 2015, 1606: 68-76.
[7] Yin YM, Lu Y, Zhang LX, et al.Bone marrow stromal cells transplantation combined with ultrashortwave therapy promotes functional recovery on spinal cord injury in rats[J]. Synapse, 2015, 69(3): 139-147.
[8] Basso DM, Beattie MS, Bresnahan JC.A sensitive and reliable locomotor rating scale for open field testing in rats[J]. J Neurotrauma, 1995, 12(1): 1-21.
[9] Anderson AJ.Mechanisms and pathways of inflammatory responses in CNS trauma: spinal cord injury[J]. J Spinal Cord Med, 2002, 25(2): 70-79.
[10] Anwar MA, Al Shehabi TS, Eid AH.Inflammogenesis of secondary spinal cord injury[J]. Front Cell Neurosci, 2016, 10: 98.
[11] Gensel JC, Zhang B.Macrophage activation and its role in repair and pathology after spinal cord injury[J]. Brain Res, 2015, 1619: 1-11.
[12] Peng Z, Gao W, Yue B, et al.Promotion of neurological recovery in rat spinal cord injury by mesenchymal stem cells loaded on nerve-guided collagen scaffold through increasing alternatively activated macrophage polarization[J]. J Tissue Eng Regen Med, 2018, 12(3): e1725-e1736.
[13] Zhao J, Wang L, Li Y.Electroacupuncture alleviates the inflammatory response via effects on M1 and M2 macrophages after spinal cord injury[J]. Acupunct Med, 2017, 35(3): 224-230.
[14] Li J, Liu Y, Xu H, et al.Nanoparticle-delivered IRF5 siRNA facilitates M1 to M2 transition, reduces demyelination and neurofilament loss, and promotes functional recovery after spinal cord injury in mice[J]. Inflammation, 2016, 39(5): 1704-1717.
[15] 孙师,万峪岑,赵利娜,等. 骨髓间充质干细胞联合超短波对大鼠脊髓损伤后早期AQP-4和GAP-43的影响[J]. 中国康复医学杂志, 2016, 31(6): 625-631.
[16] Ghosh M, Xu Y, Pearse DD.Cyclic AMP is a key regulator of M1 to M2a phenotypic conversion of microglia in the presence of Th2 cytokines[J]. J Neuroinflammation, 2016, 13: 9.
[17] Li F, Cheng B, Cheng J, et al.CCR5 blockade promotes M2 macrophage activation and improves locomotor recovery after spinal cord injury in mice[J]. Inflammation, 2015, 38(1): 126-133.
[18] Liang F, Liu M, Fu X, et al.Dexmedetomidine attenuates neuropathic pain in chronic constriction injury by suppressing NR2B, NF-κB, and iNOS activation[J]. Saudi Pharm J, 2017, 25(4): 649-654.
[19] Wang S, Ren D.Allicin protects traumatic spinal cord injury through regulating the HSP70/Akt/iNOS pathway in mice[J]. Mol Med Rep, 2016, 14(4): 3086-3092.
[20] Akgün B, Ozturk S, Artas G, et al.Effects of intrathecal caffeic acid phenethyl ester (CAPE) on IL-6 and TNF-α levels and local inflammatory responses in spinal cord injuries[J]. Turk Neurosurg, 2017. [Epub ahead of print]. doi: 10.5137/1019-5149.JTN.20011-17.1.
[21] Beattie MS.Inflammation and apoptosis: linked therapeutic targets in spinal cord injury[J]. Trends Mol Med, 2004, 10(12): 580-583.
[22] Peng RJ, Jiang B, Ding XP, et al.Effect of TNF-α inhibition on bone marrow-derived mesenchymal stem cells in neurological function recovery after spinal cord injury via the Wnt signaling pathway in a rat model[J]. Cell Physiol Biochem, 2017, 42(2): 743-752.
[23] Hao J, Li B, Duan HQ, et al.Mechanisms underlying the promotion of functional recovery by deferoxamine after spinal cord injury in rats[J]. Neural Regen Res, 2017, 12(6): 959-968.
[24] Pei JP, Fan LH, Nan K, et al.HSYA alleviates secondary neuronal death through attenuating oxidative stress, inflammatory response, and neural apoptosis in SD rat spinal cord compression injury[J]. J Neuroinflammation, 2017, 14(1): 97.

Effect of Ultrashort Wave on Macrophage Polarization and Inflammatory Response after Spinal Cord Injury in Rats

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