高压氧对大鼠脊髓损伤后蛋白激酶R样内质网激酶表达和运动功能的影响

doi:10.3969/j.issn.1006-9771.2018.12.007

Abstract

Abstract: Objective To investigate the effect of hyperbaric oxygen (HBO) on the behaviors, organizational morphology and the expression of protein kinase R-like ER kinase (PERK) in rats with spinal cord injury. Methods Thirty-six male Sprague-Dawley rats were randomly divided into normal group, sham group, model group and HBO group, with nine cases in each group. The normal group did not receive any treatment, the sham group received only laminectomy, and the other two groups were established spinal cord injury model with modified Allen's method. Six hours after operation, the model group was treated with regular air, and HBO group received HBO treatment for seven days. The recovery of locomotor function was evaluated by BBB (Basso Beattie and Bresnahan) on the day before operation and six hours, three days, seven days after operation. HE staining was used to observe the change of tissue morphology in injured spinal cord, and the expression of PERK in spinal cord was detected by Western blotting. Results There was no neurological deficit in the normal group and the sham group. The neurological score was lower in the model group than in the sham group three days and seven days after operation (P < 0.05), and was higher in HBO group than in the model group seven days after operation (P < 0.05). There was no obvious structural change in the normal group and the sham group, however, the model group showed swelling cells, condensed cytoplasm, and nucleus pycnosis hyperchromatic, and the HBO group showed slighter swelling cell compared with the model group. The expression of PERK was higher in the model group than in the sham group, and was lower in HBO group than in the model group seven days after operation (P < 0.05). Conclusion HBO could reduce the expression of PERK in the injured spinal cord, and improve the recovery of hind limb locomotor function in spinal cord injury rats.

Key words: spinal cord injury, hyperbaric oxygen, protein kinase R-like ER kinase, rats

CLC Number:

R651.2

YING Xin-wang, CHEN Xiao-long, GU Peng-peng, LI Si-si, JIANG Song-he. Effect of Hyperbaric Oxygen on Protein Kinase R-like ER Kinase Expression and Motor Function in Rats with Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(12): 1393-1397.

References

[1] Lv ZR, Liu XH.The roles of protein kinase R-like ER kinase signal pathway in endoplasmic reticulum stress[J]. [in Chinese]. Sheng Li Ke Xue Jin Zhan, 2011, 42(2): 154-157.
[2] Wang P, Li J, Tao J, et al.The luminal domain of the ER stress sensor protein PERK binds misfolded proteins and thereby triggers PERK oligomerization[J]. J Biol Chem, 2018, 293(11): 4110-4121.
[3] Scheuner D, Song B, McEwen E, et al. Translational control is required for the unfolded protein response and in vivo glucose homeostasis[J]. Mol Cell, 2001, 7(6): 1165-1176.
[4] Wang C, Guan Y, Yang J.Cytokines in the progression of pancreatic beta-cell dysfunction[J]. Int J Endocrinol, 2010, 2010: 515136.
[5] Fang F, Gong PS, Song XF, et al.Low-dose radiation induces endoplasmic reticulum stress and activates PERK-CHOP signaling pathway in mouse testicular cells[J]. [in Chinese]. Zhonghua Nan Ke Xue, 2012, 18(9): 777-782.
[6] Lee H, Noh JY, Oh Y, et al.IRE1 plays an essential role in ER stress-mediated aggregation of mutant huntingtin via the inhibition of autophagy flux[J]. Hum Mol Genet, 2012, 21(1): 101-114.
[7] He Z, Zhou Y, Huang Y, et al.Dl-3-n-butylphthalide improves functional recovery in rats with spinal cord injury by inhibiting endoplasmic reticulum stress-induced apoptosis[J]. Am J Transl Res, 2017, 9(3): 1075-1087.
[8] 于正道. 高压氧综合治疗脊髓损伤64例疗效分析[J]. 中华航海医学与高气压医学杂志, 2013, 20(6): 421-422.
[9] Tan JW, Zhang F, Liu HJ, et al.Hyperbaric oxygen ameliorated the lesion scope and nerve function in acute spinal cord injury patients: A retrospective study[J]. Clin Biochem, 2018, 53: 1-7.
[10] Khuyagbaatar B, Kim K, Kim YH.Conversion equation between the drop height in the new york university impactor and the impact force in the infinite horizon impactor in the contusion spinal cord injury model[J]. J Neurotrauma, 2015, 32(24): 1987-1993.
[11] Basso DM, Beattie MS, Bresnahan JC.A sensitive and reliable locomotor rating scale for open field testing in rats[J]. J Neurotrauma, 1995, 12(1): 1-21.
[12] Houle JD, Cote MP.Axon regeneration and exercise-dependent plasticity after spinal cord injury[J]. Ann N Y Acad Sci, 2013, 1279: 154-163.
[13] Dumont RJ, Okonkwo DO, Verma S, et al.Acute spinal cord injury, part I: pathophysiologic mechanisms[J]. Clin Neuropharmacol, 2001, 24(5): 254-264.
[14] Kwon BK, Tetzlaff W, Grauer JN, et al.Pathophysiology and pharmacologic treatment of acute spinal cord injury[J]. Spine J, 2004, 4(4): 451-464.
[15] Schilero GJ, Bauman WA, Radulovic M.Traumatic spinal cord injury: pulmonary physiologic principles and management[J]. Clin Chest Med, 2018, 39(2): 411-425.
[16] 李红玲,马维. 高压氧在骨科疾病中的应用研究进展[J]. 中国康复医学杂志, 2014, 29(10): 999-1002.
[17] 孙文志,鲁世保. 高压氧治疗脊髓损伤机制的研究进展[J]. 中国脊柱脊髓杂志 2014, 24(12): 1116-1119.
[18] Vince JE, Wong WW, Gentle I, et al.Inhibitor of apoptosis proteins limit RIP3 kinase-dependent interleukin-1 activation[J]. Immunity, 2012, 36(2): 215-227.
[19] Liu X, Yang J, Li Z, et al.Hyperbaric oxygen treatment protects against spinal cord injury by inhibiting endoplasmic reticulum stress in rats[J]. Spine (Phila Pa 1976), 2015, 40(24): E1276-E1283.
[20] Hetz C.The unfolded protein response: controlling cell fate decisions under ER stress and beyond[J]. Nat Rev Mol Cell Biol, 2012, 13(2): 89-102.
[21] Bernales S, Papa FR, Walter P.Intracellular signaling by the unfolded protein response[J]. Annu Rev Cell Dev Biol, 2006, 22: 487-508.
[22] 张嘉宾,张盼盼,王红阳,等. 磷酸化蛋白激酶样内质网激酶、C/EBP同源蛋白在慢性间歇低氧大鼠肺组织中的表达变化及意义[J]. 中国现代医学杂志, 2016, 26(15): 38-43.
[23] Liu Z, Lv Y, Zhao N, et al.Protein kinase R-like ER kinase and its role in endoplasmic reticulum stress-decided cell fate[J]. Cell Death Dis, 2015, 6: e1822.
[24] Schonthal AH.Pharmacological targeting of endoplasmic reticulum stress signaling in cancer[J]. Biochem Pharmacol, 2013, 85(5): 653-666.
[25] Hirsch I, Weiwad M, Prell E, et al.ERp29 deficiency affects sensitivity to apoptosis via impairment of the ATF6-CHOP pathway of stress response[J]. Apoptosis, 2014, 19(5): 801-815.
[26] Wang XZ, Ron D.Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase[J]. Science, 1996, 272(5266): 1347-1349.
[27] Yin J, Gu L, Wang Y, et al.Rapamycin improves palmitate-induced ER stress/NF kappa B pathways associated with stimulating autophagy in adipocytes[J]. Mediators Inflamm, 2015: 272313.
[28] Wang L, Li W, Kang Z, et al.Hyperbaric oxygen preconditioning attenuates early apoptosis after spinal cord ischemia in rats[J]. J Neurotrauma, 2009, 26(1): 55-66.

Effect of Hyperbaric Oxygen on Protein Kinase R-like ER Kinase Expression and Motor Function in Rats with Spinal Cord Injury

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LIU Dong, XU Zihan, LI Jiang, JU Ping. Effect of high-frequency repetitive transcranial magnetic stimulation in M1 region combined with dorsolateral prefrontal cortex on electroencephalogram θ frequency band amplitude of patients with neuropathic pain after spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2024, 30(1): 87-94.
[2]	SUN Tengfang, REN Mengting, YANG Lin, WANG Yaoting, WANG Hongyu, YAN Xingzhou. Effect of hyperbaric oxygen therapy combined with repetitive peripheral magnetic stimulation on ankle motor function and balance of stroke patients [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(8): 875-881.
[3]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[4]	LIU Ning, LIU Yuquan, ZHU Bin, YU Lingjia, TAN Haining, YANG Yong, LI Xiang. Application of International Standards for Neurological Classification of Spinal Cord Injury in China: a bibliometrics re-analysis [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 808-815.
[5]	WANG Yiji, ZHOU Hongjun, HE Zejia, LIU Genlin, ZHENG Ying, HAO Chunxia, WEI Bo, KANG Haiqiong, ZHANG Ying, LU Xiaolei, YUAN Yuan, MENG Qianru. Relationship between symmetry of lower limb function and gait symmetry in patients with incomplete spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(6): 639-645.
[6]	YUAN Yuan, ZHOU Hongjun, CONG Xinying, LIU Genlin, WEI Bo, ZHENG Ying, HAO Chunxia, ZHANG Ying, WANG Yiji, KANG Haiqiong, LU Xiaolei, MENG Qianru. Relationship between impairment and magnetic resonance imaging finding in patients with traumatic cervical spinal cord injury after surgery [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(6): 725-730.
[7]	JIANG Le, DU Liangjie, HUANG Fubiao. Mood states and cognitive performance in patients with complete spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 576-581.
[8]	GUO Shuang, XIE Yongqi, ZHANG Liang, ZHANG Chunjia, PENG Run, YANG Degang, YANG Mingliang. Related factors and prediction model for neurological outcome of dance-associated pediatric spinal cord injury without radiographic abnormality [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 582-589.
[9]	ZHANG Yuan, YANG Jian. Exercise rehabilitation interventions for people with spinal cord injury and their health benefits: a systematic review of systematic reviews based on ICD-11 and ICF [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(12): 1377-1385.
[10]	SHI Xiaoyu, YANG Jian. Adaptive physical activity and its health benefits for patients with spinal cord injury based on ICF: a scoping review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(12): 1395-1404.
[11]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[12]	YUAN Huiping, FENG Xiaojun, JIANG Dongsheng, CHE Xingwang, FAN Lianbin. Effect of hyperbaric oxygen combined with dynamic scalp acupuncture on motor function for stroke patients with hemiplegia [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1208-1213.
[13]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[14]	LIU Genlin,ZHOU Hongjun,LI Jianjun,WEI Bo,ZHENG Ying,HAO Chunxia,ZHANG Ying,WANG Yiji,KANG Haiqiong,LU Xiaolei,YUAN Yuan,MENG Qianru. Advance in neurological classification of spinal cord injury with complications [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(8): 934-938.
[15]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.