大鼠脊髓突触体中生理活性物质分析

doi:10.3969/j.issn.1006-9771.2018.12.008

Abstract

Abstract: Objective To analyze the physiologically active substances, named amino acid neurotransmitters, ATP and Ca²⁺ in rat spinal synaptosomes. Methods The spinal synaptosome was extracted by discontinuous Percoll density gradient centrifugation from a female Sprague-Dawley rat. The amino acid neurotransmitters were determined with high performance liquid chromatography, ATP content, Na⁺-K⁺-ATPase activity, Ca²⁺-Mg²⁺-ATPase activity and total ATPase activity with ultraviolet spectrophotometer, and Ca²⁺ concentration with fluorescence spectrophotometer. Results The amino acid neurotransmitters contained mainly aspartic acid, glycine, glutamic acid and r-aminobutyric acid. The concentration of ATP was about 414.7461 μmol/mg, total ATPase activity > Na⁺-K⁺-ATPase activity > Ca²⁺-Mg²⁺-ATPasese activity. The concentration of Ca²⁺ could be calculated from absorbancy directly. Conclusion The rat spinal synaptosome contains a variety of physiologically active substances, which can be accurately analyzed to explore their activities.

Key words: spinal cord, synaptosomes, amino acid neurotransmitters, adenosine triphosphate, calcium ion, rats

CLC Number:

R338.2

LIU Zheng, YUAN Wen-hua, ZHAO Shu-xuan, SHEN Wei, FENG Xiao-fei, ZHOU Hai-yu. Analysis of Physiologically Active Substances in Rat Spinal Synaptosome[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(12): 1398-1402.

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Analysis of Physiologically Active Substances in Rat Spinal Synaptosome

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