“三法三穴”推拿手法对坐骨神经损伤大鼠运动功能和脊髓NogoA及其受体表达的影响

doi:10.3969/j.issn.1006-9771.2018.12.009

Abstract

Abstract: Objective To investigate the effects of Tuina of Three Handing-Three Points on hindlimb motor function and expression of protein NogoA and its receptor NgR in the spinal cord of rats with sciatic nerve injury. Methods A total of 64 male Sprague-Dawley rats were randomly assigned to normal group (n = 16), sham group (n = 16), model group (n = 16) and Tuina group (n = 16). The model group and Tuina group prepared the sciatic nerve injury model with brace method. Tuina group received Tuina in methods of pressing, strumming and circular rubbing on Yinmen (BL37), Chengshan (BL57) and Yanglingquan (GB34) with Tuina manipulation emulator. The rats were assessed with oblique plate test seven days after operation and 20 days after Tuina. Then, the spinal cord tissues were extracted to detect the expression of protein of NogoA and NgR with Western blotting. Results The scores of the oblique plate test decreased in the model group and Tuina group seven days after operation compared with those in the normal group (P < 0.05), while the expression NgR increased (P < 0.05). The scores of the oblique plate test increased in Tuina group compared with those in the model group 20 days after Tuina (P < 0.05), while the expression of NgR decreased (P < 0.05), similar to the normal group (P > 0.05). Conclusion Tuina of Three Handing-Three Points can improve the hindlimb motor function in rats with sciatic nerve injury, which may be related to inhibition of the expression of NgR in the spinal cord after sciatic nerve injury.

Key words: sciatic nerve injury, Tuina, NogoA, receptor, nerve regeneration, rats

CLC Number:

R745.4

LI Yi-zhen, YANG Chao, YU Tian-yuan, LU Meng-qian, MIAO Run-pei, LÜ Tao-tao. Effects of Tuina of Three Handing-Three Points on Motor Function and Expression of NogoA and Its Receptor in Spinal Cord of Rats with Sciatic Nerve Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(12): 1403-1407.

References

[1] 姚斌彬,梅旭晖,吴剑聪,等. 基于马达蛋白探讨推拿对坐骨神经损伤大鼠轴浆运输功能的影响[J]. 南京中医药大学学报, 2013, 29(4): 338-341.
[2] 吴凡,张蓓,郑慧敏,等. 推拿治疗坐骨神经损伤大鼠行为学研究[J]. 成都中医药大学学报, 2013, 36(1): 41-43.
[3] 卢新刚,喻立炜,苟海昕,等. 一指禅推拿对坐骨神经损伤大鼠神经形态及功能的影响[J]. 中国中医药信息杂志, 2017, 24(9): 35-38.
[4] 陈德英,程赛宇,李成仁. 坐骨神经损伤与修复过程中相应脊髓神经元变化的形态学观察[J]. 中国临床康复, 2002, 6(8): 1119-1120.
[5] 刘仁红,周晓光. Nogo蛋白及其受体在中枢神经再生中的作用[J]. 国际儿科学杂志, 2006, 33(2): 111-113.
[6] 李忠仁. 实验针灸学[M]. 北京:中国中医药出版社, 2003: 147-148.
[7] 白丽敏,姜国华. 神经解剖学[M]. 北京:中国中医药出版社, 2011: 38.
[8] Tom VJ, Sandrowfeinberg HR, Miller K, et al.Combining peripheral nerve grafts and chondroitinase promotes functional axonal regeneration in the chronically injured spinal cord[J]. J Neurosci, 2011, 29(47): 14881-14890.
[9] Raiker SJ, Lee H, Baldwin KT, et al.Oligodendrocyte-myelin glycoprotein and Nogo negatively regulate activity-dependent synaptic plasticity[J]. J Neurosci, 2010, 30(37): 12432-12445.
[10] 毕小彬,邓宇斌,王亚柱. 髓鞘相关抑制因子与中枢神经系统损伤[J]. 国际病理科学与临床杂志, 2006, 26(4): 343-346.
[11] Fournier AE, Grandpre T, Strittmatter SM.Identification of a receptor mediating Nogo-66 inhibition of axonal regeneration[J]. Nature, 2001, 409(6818): 341-346.
[12] Hunt D, Mason MR, Campbell G, et al.Nogo receptor mRNA expression in intact and regenerating CNS neurons[J]. Mol Cell Neurosci, 2002, 20(4): 537-552.
[13] Liu BP, Fournier A, GrandPré T, et al. Myelin-associated glycoprotein as a functional ligand for the Nogo-66 receptor[J]. Science, 2002, 297(5584): 1190-1193.
[14] Schwab ME.Functions of Nogo proteins and their receptors in the nervous system[J]. Nat Rev Neurosci, 2010, 11(12): 799-811.
[15] Wang KC, Kim JA, Sivasankaran R, et al.P75 interacts with the Nogo receptor as a co-receptor for Nogo, MAG and OMgp[J]. Nature, 2002, 420(6911): 74-78.
[16] Mi S, Lee X, Shao Z, et al.LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex[J]. Nat Neurosci, 2004, 7(3): 221.
[17] 王峻,刘爱香,秦宁,等. Nogo受体拮抗剂对新生大鼠缺氧缺血性脑损伤神经修复作用的研究[J]. 当代医学, 2012, 18(19): 4-6.
[18] Gou X, Wang Q, Yang Q, et al.TAT-NEP1-40 as a novel therapeutic candidate for axonal regeneration and functional recovery after stroke[J]. J Drug Target, 2011, 19(2): 86-95.
[19] Cao Y, Shumsky JS, Sabol MA, et al.Nogo-66 receptor antagonist peptide (NEP1-40) administration promotes functional recovery and axonal growth after lateral funiculus injury in the adult rat[J]. Neurorehab Neural Repair, 2008, 22(3): 262-278.
[20] 李鹏,许玉本,夏雷,等. 新型Nogo受体拮抗剂A3NEP1-40对大鼠坐骨神经损伤的保护作用[J]. 美中国际创伤杂志, 2015(4): 1-7.
[21] 闵友江,孙洁,贾永忠,等. 脊髓损伤大鼠NogoA、NgR mRNA和蛋白的时相表达及电针治疗时间窗[J]. 中国康复理论与实践, 2018, 24(6): 621-628.
[22] 李小琴,高玉峰,于跃,等. 电针对大鼠脊髓中抑制性生长因子的影响[J]. 环球中医药, 2013, 6(9): 653-657.
[23] 鲁梦倩,于天源,姚斌彬,等. 推拿对坐骨神经损伤模型大鼠神经超微结构的影响[J]. 南京中医药大学学报, 2015, 31(4): 349-352.
[24] 梅旭晖,纪倩,姚斌彬,等. 推拿对坐骨神经损伤大鼠神经生长因子及其受体p75NTR的影响[J]. 中华中医药杂志, 2013, 28(7): 1994-1997.
[25] 高玉峰,姚斌彬,吴剑聪,等. 推拿对大鼠坐骨神经损伤后MAP-2和NF-M表达的影响[J]. 中医药导报, 2014, 20(1): 11-13.
[26] Pan F, Yu TY, Wong S, et al.Chinese Tuina downregulates the elevated levels of tissue plasminogen activator in sciatic nerve injured Sprague-Dawley rats[J]. Chin J Integr Med, 2017, 23(8): 1-8.
[27] 高玉峰. 神经元细胞骨架蛋白在推拿治疗坐骨神经损伤中的作用及机理探讨[D]. 北京:北京中医药大学, 2014.

Effects of Tuina of Three Handing-Three Points on Motor Function and Expression of NogoA and Its Receptor in Spinal Cord of Rats with Sciatic Nerve Injury

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[2]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[3]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[4]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.
[5]	ZHOU Xiaojue,FENG Jing,PANG Rizhao,LIU Jie,ZHANG Anren. Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 544-551.
[6]	SONG Shaofei,HOU Yuanyuan,WANG Yunlei,ZHANG Tong. Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 552-558.
[7]	LI Tong,FANG Zhipeng,SHAO Yuping,WANG Ping. Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1270-1277.
[8]	YIN Tao,ZHENG Zuncheng,GAO Qiang. Effect of sling exercise therapy combined with Tuina on nerve conduction of upper extremities after radicular cervical spondylosis [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(1): 95-99.
[9]	Jing-yi WANG,Jie YIN,Jian-cheng LIU,Ri-zhao PANG,Wen-chun WANG. Effect of Iridoid-rich Fraction from Valeriana Jatamansi Jones on Neuron Pyroptosis in Rats with Acute Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 653-660.
[10]	Wen-mei ZHOU,Tao TAO,Shuang WU,Ting-long WANG,Zheng-yi YANG,Ying ZHANG. Effects of Enriched Environment on Neurological Function and Glucose Metabolism in Ischemic Penumbra in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(5): 522-529.
[11]	Qiong-fen WANG,Feng-bo WANG,Ke WANG,Yong-qiang ZHONG,Jiao-jiao WANG. Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(3): 302-309.
[12]	Xiang-zhe LI,Jie DING,Qing-hua WANG,Chuan-ming DONG,Tong WANG,Qin-feng WU. Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase (GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 131-136.
[13]	Kun AI,Ming XU,Qiong LIU,Shi-feng DENG,Ji-sheng LIU,Fang QI,Xi-qin YI,Qi-rui QU,Hong ZHANG. Effect of Electroacupuncture on Content of Cyclic Adenosine Monophosphate and Protein Kinase A, and Phosphorylation of Myosin Light Chain Kinase in Detrusor of Rats with Detrusor Hyperreflex after Suprasacral Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 137-144.
[14]	YANG He,TANG Qiang,ZHU Lu-wen. Effect of Acupuncture-rehabilitation Therapy on Neural Function and Expression of Forkhead Box P3 and Retinoic Acid-related Orphan Receptor γt in Mice with Cerebral Ischemia [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(12): 1437-1442.
[15]	LING Meng-yu,YANG Yi-zhuo,LIU Shuai,YE Chao-qun. Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(11): 1272-1281.