超短波对急性肺损伤大鼠炎症反应的疗效及其机制

doi:10.3969/j.issn.1006-9771.2020.00.019

Abstract

Abstract:

Objective To observe the effects of ultrashort wave (USW) on lipopolysaccharide (LPS) induced acute lung injury (ALI) and nucleotide-binding oligomerization domain like receptor protein 3 (NLRP3) signaling pathway in rats.
Methods Twenty-four three-month-old male Sprague-Dawley rats were randomly divided into control group (n = 8), ALI group (n = 8) and USW group (n = 8). The ALI and USW groups were instilled with LPS to induce ALI, and the USW group was treated with ultrashort wave 0, four and eight hours after instillation, 15 minutes a time. Twenty-four hours after instillation, the lung tissue of the rats was measured the wet/dry mass ratio (W/D), and observed under HE staining. Serum levels of interleukin (IL)-1β and IL-18 were detected with ELISA. The mRNA and protein expression of NLRP3, caspase-1 and IL-1β in the lung tissue were detected with reverse transcription polymerase chain reaction and Western blotting, respectively.
Results W/D increased in ALI group compared with that of the control group (P < 0.05), and it decreased in USW group without significance compared with that of ALI group ( P > 0.05). Lung injury score increased in ALI group compared with that of the control group ( P < 0.05), and it decreased in USW group compared with that of ALI group ( P < 0.05); as well as the serum IL-1β and IL-18, and mRNA and protein expression of NLRP3, caspase-1 and IL-1β.
Conclusion USW can alleviate the inflammatory of acute lung injury, which may associate with inhibiting of NLRP3 signaling pathway.

Key words: acute lung injury, ultrashort wave, inflammatory, nucleotide-binding oligomerization domain like receptor protein 3, signaling pathway, rats

CLC Number:

R563.1

ZHOU Jun,LIU Dan-ni,WANG Li-qiong,WANG Qian,LI Yi,QU Meng-jian,LIU Jing,ZENG Ya-hua,HE Cheng-qi. Effects of Ultrashort Wave on Inflammatory of Acute Lung Injury and Related Signaling Pathway in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(10): 1176-1181.

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References 41

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组别	n	W/D	肺损伤评分	IL-1β (pg/ml)	IL-18 (pg/ml)
对照组	8	1.97±0.72	1.25±0.46	22.41±6.56	137.50±59.70
ALI组	8	4.42±0.71^a	10.38±1.30^a	42.88±9.64^a	436.25±136.17^a
超短波组	8	4.12±1.27	3.75±0.71^b	31.36±9.03^b	250.00±73.48^b
F值		14.205	221.356	10.818	18.972
P值		< 0.001	< 0.001	0.001	< 0.001

组别	n	NLRP3	caspase-1	IL-1β
对照组	8	0.80±0.34	1.25±0.26	0.96±0.21
ALI组	8	3.88±1.27^a	11.74±2.68^a	12.91±5.90^a
超短波组	8	1.70±0.45^b	4.89±1.64^b	1.50±1.02^b
F值		23.266	51.361	22.832
P值		< 0.001	< 0.001	< 0.001

组别	n	NLRP3	caspase-1	IL-1β
对照组	8	0.16±0.04	0.11±0.05	0.16±0.06
ALI组	8	0.37±0.02^a	0.26±0.02^a	0.46±0.06^a
超短波组	8	0.20±0.05^b	0.20±0.05^c	0.30±0.12^b
F值		54.580	19.328	18.361
P值		< 0.001	< 0.001	< 0.001

Effects of Ultrashort Wave on Inflammatory of Acute Lung Injury and Related Signaling Pathway in Rats

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