大鼠骨骼肌急性损伤后早期运动训练和按摩对肌卫星细胞增殖相关因子的影响

doi:10.3969/j.issn.1006-9771.2020.01.008

Abstract

Abstract:

Objective To study the effect of treadmill exercise and massage shortly after acute injury on expression of key growth factor in muscle satellite cells activation, insulin-like growth factor-1 (IGF-1), and mitogen-activated protein kinase (MAPK) / mitogen-activated protein kinase kinase (MEK) / extracellular regulated protein kinases (ERK)1/2 signaling pathways in muscle satellite cell in rats.
Methods A total of 40 SPF male adult Sprague-Dawley rats were randomly divided into groups A (n = 8), B (n = 8), C (n = 8), D (n = 8) and E (n = 8). Group A did not receive any treatment, while the other rats were contused the gastrocnemius muscle with self-made impactor. Group B received no intervention, groups C and D received massage and treadmill exercise shortly after injury, respectively, while group E received both treadmill exercise and massage shortly after injury. As the model was established, samples of gastrocnemius were obtained from all the rats 24 hours after injury, and observed under HE staining, detected the expression of MyoD1 and MyoG mRNA with reverse transcription polymerase chain reaction (RT-PCR), and expression of IGF-1, p-MAPK, p-MEK and p-ERKI/2 protein with Western blotting.
Results The expression of MyoD1 mRNA was more in groups C, D and E than in group B, which was the most in group C; less expression of MyoG mRNA, which was the most in group E (P < 0.05). The expression of p-MAPK, p-MEK and p-EPK1/2 was more in groups C, D and E than in group B, which was the most in group D ( P < 0.05). The expression of IGF-1 increased in group C compared with that in group B ( P < 0.05), and it decreased in group D ( P < 0.05).
Conclusion Early intervention of treadmill exercise and massage may promote the activation of muscle satellite cells in different ways.

Key words: acute skeletal muscle injury, treadmill exercise, massage, muscle satellite cells, rats

CLC Number:

R685.5

HE Zhou,CHANG Qing,TANG Cheng-lin,ZHU Zheng-wei. Effect of Early Treadmill Exercise and Massage on Activative Factors of Muscle Satellite Cells after Acute Injury of Skeletal Muscle in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(1): 49-54.

Figures/Tables 4

References 38

[1]	张安宁. PI3K/Akt/mTOR信号通路在大鼠急性骨骼肌钝挫伤[J]. 中国运动医学杂志, 2018, 37(7):594-600.
[2]	李同辉. 运动员骨骼肌钝挫伤的研究进程[J]. 当代体育科技, 2013, 3(12):29-31.
[3]	杨桂其, 史传华, 朱小军, 等. 大学生体育运动损伤的预防与处理[J]. 体育世界(学术版), 2018(11):171-176.
[4]	杨辉, 唐成林, 常青, 等. 在大鼠骨骼肌急性损伤修复过程中按摩对神经型一氧化氮合成酶、肝细胞生长因子mRNA表达的影响[J]. 中国康复医学杂志, 2015, 30(3):224-230.
[5]	郭全虎. 24小时内推拿对骨骼肌急性钝挫伤模型大鼠肌细胞膜修复相关蛋白dysferlin表达的影响[J]. 中国康复医学杂志, 2018, 33(2):151-157.
[6]	高尚, 唐康来, 张吉强, 等. 不同强度跑台训练对大鼠跟腱微损伤修复影响的实验研究[J]. 中国修复重建外科杂志, 2017, 31(5):574-581.
[7]	胡劲松, 李飞跃. 冰敷和消肿散外敷对损伤骨骼肌IGF-1 mRNA表达及组织形态的影响[J]. 上海中医药杂志, 2018, 52(9):74-81.
[8]	屈伟, 傅继华. 骨骼肌损伤的病理进程及治疗措施的研究进展[J]. 北方药学, 2015, 12(3):118-119.
[9]	Bleakley C M, Glasgow P, MacAuley D C. PRICE needs updating, should we call the POLICE?[J]. Br J Sports Med, 2012, 46(4):220-221. doi: 10.1136/bjsports-2011-090297
[10]	李长飞, 张建礼. 急性软组织损伤处理原则的思考[J]. 福建体育科技, 2018, 37(3):41-43.
[11]	Bleakley Chris M, O'Connor Seán R, Tully Mark A, et al. Effect of accelerated rehabilitation on function after ankle sprain: randomised controlled trial[J]. BMJ, 2010, 340:c1964. doi: 10.1136/bmj.c1964
[12]	Tedesco F S, Dellavalle A. Repairing skeletal muscle: regenerativ potential of skeletal muscle stem cells[J]. Clin Invest, 2010, 120(1):11-19. doi: 10.1172/JCI40373
[13]	Tidball J G. Mechanisms of muscle injury, repair, and regeneration[J]. Compr Physiol, 2011, 1(4):2029-2062. doi: 10.1002/cphy.c100092 pmid: 23733696
[14]	刘晓光, 肖卫华, 赵淋淋, 等. 间充质干细胞移植治疗骨骼肌损伤的研究进展[J]. 中国康复医学杂志, 2015, 30(12):1313-1317.
[15]	刘晓光, 赵淋淋, 曾志刚, 等. 骨骼肌损伤修复过程中肌再生调节因子和血管再生因子表达规律研究[J]. 中国康复医学杂志, 2016, 31(12):1294-1300.
[16]	Hill M, Wernig A, Goldspink G. Muscle satellite (stem) cell activation during local tissue injury and repair[J]. J Anat, 2003, 203(1):89-99. doi: 10.1046/j.1469-7580.2003.00195.x
[17]	孙婧瑜, 陆耀飞. 机械生长因子对骨骼肌卫星细胞的调控[J]. 中国组织工程研究与临床康复, 2010, 14(20):3740-3743.
[18]	Charge S B, Rudnicki M A. Cellular and molecular regulation of muscle regeneration[J]. Physiol Rev, 2004, 84(1):209-238. doi: 10.1152/physrev.00019.2003
[19]	肖卫华, 陈佩杰, 刘宇. 巨噬细胞在骨骼肌急性损伤修复中的作用研究进展[J]. 中国运动医学杂志, 2014(3):269-274.
[20]	Zanou N, Gailly P. Skeletal muscle hypertrophy and regeneration: interplay between the myogenic regulatory factors (MRFs) and insulin-like growth factors (IGFs) pathways[J]. Cell Mol Life Sci, 2013, 70(21):4117-4130. doi: 10.1007/s00018-013-1330-4
[21]	郑莉芳, 陈佩杰, 周永战, 等. 老年骨骼肌再生能力受损的机制研究进展[J]. 生理科学进展, 2017, 48(5):393-397.
[22]	Bikle D D, Tahimic C, Chang W, et al. Role of IGF-I signaling in muscle bone interactions[J]. Bone, 2015, 80:79-88. doi: 10.1016/j.bone.2015.04.036
[23]	杨奕. 成年大鼠骨骼肌成肌细胞的培养和鉴定[J]. 哈尔滨医科大学学报, 2009, 43(2):137-139.
[24]	Luo W, Li E, Nie Q, et al. Myomaker, Regulated by MYOD, MYOG and miR-140-3p, promotes chicken myoblast fusion[J]. Int J Mol Sci, 2015, 16(11):26186-26201. doi: 10.3390/ijms161125946
[25]	Hodgson B, Mafi R, Mafi P, et al. The regulation of differentiation of mesenchymal stem-cells into skeletal muscle: a look at signalling molecules involved in myogenesis[J]. Curr Stem Cell Res Ther, 2018, 13(5):384-407. doi: 10.2174/1574888X11666170907113151
[26]	Knapp J R, Davie J A, Meadows E, et al. Loss of myogenin in postnatal life leads to normal skeletal muscle but reduced body size[J]. Development, 2006, 133(4):601-610.
[27]	曾缨, 张成, 刘克玄, 等. 成肌调节因子MyoD与myogenin在肌肉损伤修复过程的动态变化[J]. 南方医科大学学报, 2004, 24(5):542-545.
[28]	侯懿烜, 柳满然, 张萍, 等. 骨骼肌损伤修复的生理生化机制研究[J]. 激光生理学报, 2011, 20(5):662-667.
[29]	杨辉, 常青, 唐成林, 等. 跑台运动训练与按摩联合作用对大鼠骨骼肌急性损伤修复过程中炎症的发展及肌卫星细胞增殖的影响[J]. 体育科学, 2015, 35(3):51-58.
[30]	张安宁, 黄思琴, 唐成林, 等. 电针联合跑台训练对大鼠骨骼肌钝挫伤的修复作用[J]. 针刺研究, 2017, 42(5):391-396.
[31]	侯懿烜, 郑元义, 张萍, 等. 按摩增强琥珀酸脱氢酶和钾-钠ATP酶活性促进兔股四头肌的损伤修复[J]. 第三军医大学学报, 2011, 33(19):2012-1015.
[32]	Kraener R. Response of IGF-I to endogenous increases in growth hormone after heavy restance exercise[J]. J Appl Physiol, 1995, 79(4):1310-1315. doi: 10.1152/jappl.1995.79.4.1310
[33]	史仍飞, 危小焰. 机械生长因子(MGF)与运动[J]. 体育科学, 2006, 26(6):90-92.
[34]	Haddad F, Adams G R. Selected contribution: acute cellular and molecular response to resistance exercise[J]. J Appl Physiol, 2002, 93(1):394-403. pmid: 12070230
[35]	刘晓光, 徐苗苗, 陈佩杰, 等. IGF-1在治疗骨骼肌损伤中的应用潜能及其相关机制[J]. 生命的化学, 2016, 36(4):496-502.
[36]	Muller A, Thelen M H, Zuidwijk M J, et al. Expression of MyoD in cultured primary myotubes is dependent on contractile activity: correlation with phenotype-specific expression of a sarcoplasmic reticulum Ca²+-ATPase isoform[J]. Biochem Biophys Res Commun, 1996, 229(1):198-204. doi: 10.1006/bbrc.1996.1780
[37]	Sakuma K, Watanabe K, Sano M, et al. The adaptive response of MyoD family proteins in overloaded, regenerating and denervated rat muscles[J]. Biochim Biophys Acta, 1999, 1428( 1999):284-292.
[38]	Hämäläinen N, Pette D. The histochemical profiles of fast fiber types IIB, IID, and IIA in skeletal muscles of mouse, rat, and rabbit[J]. J Histochem Cytochem, 1993, 41(5):733-743. pmid: 8468455

组别	n	IGF-1	p-MAPK	p-MEK	P-ERK1/2
A组	8	1.089±0.036	0.678±0.040	0.404±0.024	0.370±0.014
B组	8	0.832±0.057^a	0.439±0.056^a	0.343±0.003^a	0.274±0.016^a
C组	8	0.938±0.065^a,b	0.669±0.015^b	0.619±0.015^a,b	0.633±0.043^a,b
D组	8	0.759±0.045^a,b,c	1.067±0.037^a,b,c	0.917±0.019^a,b,c	1.022±0.051^a,b,c
E组	8	0.844±0.036^a,c,d	0.749±0.04^a,b,c,d	0.506±0.035^a,b,c,d	0.855±0.057^a,b,c,d
F值		53.357	256.328	847.419	493.807
P值		< 0.001	< 0.001	< 0.001	< 0.001

组别	n	MyoD1	MyoG
A组	8	0.417±0.010	0.175±0.006
B组	8	0.445±0.007^a	0.234±0.007^a
C组	8	0.493±0.008^a,b	0.208±0.003^a,b
D组	8	0.480±0.011^a,b,c	0.216±0.002^a,b,c
E组	8	0.464±0.009^a,b,c,d	0.222±0.005^a,b,c,d
F值		87.458	169.181
P值		< 0.001	< 0.001

Effect of Early Treadmill Exercise and Massage on Activative Factors of Muscle Satellite Cells after Acute Injury of Skeletal Muscle in Rats

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 38

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[2]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[3]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[4]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.
[5]	ZHOU Xiaojue,FENG Jing,PANG Rizhao,LIU Jie,ZHANG Anren. Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 544-551.
[6]	SONG Shaofei,HOU Yuanyuan,WANG Yunlei,ZHANG Tong. Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 552-558.
[7]	LI Zewen,ZHANG Peixiao,LI Li. Effect of sling-massage exercise on upper limb spasticity for stroke patients at recovery stage [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1252-1258.
[8]	LI Tong,FANG Zhipeng,SHAO Yuping,WANG Ping. Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1270-1277.
[9]	LI Jian-fei,LI Qian,LI Jia-lu,WANG Jian,LIU Li,LI Li. Long-term Effect of Sling-massage Exercise on Cervical Spondylopathy [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(7): 834-839.
[10]	Jing-yi WANG,Jie YIN,Jian-cheng LIU,Ri-zhao PANG,Wen-chun WANG. Effect of Iridoid-rich Fraction from Valeriana Jatamansi Jones on Neuron Pyroptosis in Rats with Acute Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 653-660.
[11]	Wen-mei ZHOU,Tao TAO,Shuang WU,Ting-long WANG,Zheng-yi YANG,Ying ZHANG. Effects of Enriched Environment on Neurological Function and Glucose Metabolism in Ischemic Penumbra in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(5): 522-529.
[12]	Qiong-fen WANG,Feng-bo WANG,Ke WANG,Yong-qiang ZHONG,Jiao-jiao WANG. Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(3): 302-309.
[13]	Xiang-zhe LI,Jie DING,Qing-hua WANG,Chuan-ming DONG,Tong WANG,Qin-feng WU. Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase (GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 131-136.
[14]	Kun AI,Ming XU,Qiong LIU,Shi-feng DENG,Ji-sheng LIU,Fang QI,Xi-qin YI,Qi-rui QU,Hong ZHANG. Effect of Electroacupuncture on Content of Cyclic Adenosine Monophosphate and Protein Kinase A, and Phosphorylation of Myosin Light Chain Kinase in Detrusor of Rats with Detrusor Hyperreflex after Suprasacral Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 137-144.
[15]	LING Meng-yu,YANG Yi-zhuo,LIU Shuai,YE Chao-qun. Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(11): 1272-1281.