水通道蛋白过表达对脊髓损伤大鼠便秘的影响

doi:10.3969/j.issn.1006-9771.2020.02.008

Abstract

Abstract:

Objective To investigate the relation between aquaporins (AQPs) and fecal water content in rats with spinal cord injury.Methods A total of 48 female Sprague-Dawley rats were divided into control group (n= 24) and spinal cord injury group (SCI group, n = 24). SCI group underwent transection at T₈, while the control group was only subjected to laminectomy. Posterior limb function was assessed by Basso-Beattie-Bresnahan (BBB) score before modeling and on the 1st, 3rd, 7th, 14th and 28th day after SCI. Fecal water content was measured before modeling and on the 3rd, 14th and 28th day after SCI. Colon specimens were collected to detect the expression of AQP1, AQP3 and AQP4 by immunohistochemistry on the 3rd, 14th and 28th day after SCI.Results The BBB score was significantly lower in SCI group than in the control group (t > 69.230, P< 0.001) after SCI, as well as the fecal water content (t > 5.814, P< 0.001). The expression of AQP1, AQP3, and AQP4 in the colon was higher in SCI group than in the control group (|t|> 5.165,P < 0.01) on the 3rd, 14th, and 28th day after SCI. The expression of AQPs negatively correlated with fecal water content ( r = -0791~-0.730, P< 0.001).Conclusion The expression of AQPs in the colon of rats after SCI was up-regulated, which was correlated with excessive water absorption after SCI.

Key words: spinal cord injury, bowel dysfunction, aquaporin, constipation, colon, fecal water content, rats

CLC Number:

R651.2

XU Pei-pei,YANG Ming-liang,LIU Chang-bin,LI Ya-jing,LIU Zi-tong,LI Jian-jun. Aquaporin Over-expression for Constipation in Rats with Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(2): 181-188.

Figures/Tables 11

References 44

[1]	Gamblin A, Garry J G, Wilde H W, et al. Cost analysis of inpatient rehabilitation after spinal injury: a retrospective cohort analysis[J]. Cureus, 2019, 11(9):e5747.
[2]	Kim J E, Go J, Sung J E, et al. Uridine stimulate laxative effect in the loperamide-induced constipation of SD rats through regulation of the mAChRs signaling pathway and mucin secretion[J]. BMC Gastroenterol, 2017, 17(1):21. doi: 10.1186/s12876-017-0576-y
[3]	Ikarashi N, Nagoya C, Kon R, et al. Changes in the expression of aquaporin-3 in the gastrointestinal tract affect drug absorption[J]. Int J Mol Sci, 2019, 20(7):E1559.
[4]	Zhu S, Ran J, Yang B, et al. Aquaporins in digestive system[J]. Adv Exp Med Biol, 2017, 969:123-130.
[5]	Peplowski M A, Dicay M, Baggio C H, et al. Interferon gamma decreases intestinal epithelial aquaporin 3 expression through downregulation of constitutive transcription[J]. J Mol Med (Berl), 2018, 96(10):1081-1093. doi: 10.1007/s00109-018-1681-2 pmid: 30090948
[6]	Zhu X, Shen W, Wang Y, et al. Nicotinamide adenine dinucleotide replenishment rescues colon degeneration in aged mice[J]. Signal Transduct Target Ther, 2017, 2:17017. doi: 10.1038/sigtrans.2017.17
[7]	Ikarashi N, Baba K, Ushiki T, et al. The laxative effect of bisacodyl is attributable to decreased aquaporin-3 expression in the colon induced by increased PGE2 secretion from macrophages[J]. Am J Physiol Gastrointest Liver Physiol, 2011, 301(5):G887-G895. doi: 10.1152/ajpgi.00286.2011
[8]	Ikarashi N, Kon R, Iizasa T, et al. Inhibition of aquaporin-3 water channel in the colon induces diarrhea[J]. Biol Pharm Bull, 2012, 35(6):957-962. doi: 10.1248/bpb.35.957
[9]	Vitores A A, Sloley S S, Martinez C, et al. Some autonomic deficits of acute or chronic cervical spinal contusion reversed by interim brainstem stimulation[J]. J Neurotrauma, 2018, 35(3):560-572. doi: 10.1089/neu.2017.5123
[10]	Ambalayam S, Jain S, Mathur R. Abnormal feeding behaviour in spinalised rats is mediated by hypothalamus: Restorative effect of exposure to extremely low frequency magnetic field[J]. Spinal Cord, 2016, 54(12):1076-1087. doi: 10.1038/sc.2016.32 pmid: 27163452
[11]	Yoshiyama M, de Groat W C. Effect of bilateral hypogastric nerve transection on voiding dysfunction in rats with spinal cord injury[J]. Exp Neurol, 2002, 175(1):191-197. doi: 10.1006/exnr.2002.7887
[12]	Diogo C C, da Costa L M, Pereira J E, et al. Kinematic and kinetic gait analysis to evaluate functional recovery in thoracic spinal cord injured rats[J]. Neurosci Biobehav Rev, 2019, 98:18-28. doi: 10.1016/j.neubiorev.2018.12.027
[13]	Cavalcante M T, Cavalcante L S, Bezerra C K, et al. Mangiferin, a natural xanthone, accelerates gastrointestinal transit in mice involving cholinergic mechanism[J]. World J Gastroenterol, 2012, 18(25):3207-3214.
[14]	Fei G, Raehal K, Liu S, et al. Lubiprostone reverses the inhibitory action of morphine on intestinal secretion in guinea pig and mouse[J]. J Pharmacol Exp Ther, 2010, 334(1):333-340. doi: 10.1124/jpet.110.166116
[15]	Patel S P, Smith T D, VanRooyen J L, et al. Serial diffusion tensor imaging in vivo predicts long-term functional recovery and histopathology in rats following different severities of spinal cord injury[J]. J Neurotrauma, 2016, 33(10):917-928. doi: 10.1089/neu.2015.4185
[16]	Liu C B, Yang D G, Zhang X, et al. Degeneration of white matter and gray matter revealed by diffusion tensor imaging and pathological mechanism after spinal cord injury in canine[J]. CNS Neurosci Ther, 2019, 25(2):261-272. doi: 10.1111/cns.2019.25.issue-2
[17]	Loo D D, Wright E M, Zeuthen T. Water pumps[J]. J Physiol, 2002, 542(Pt 1):53-60. doi: 10.1113/jphysiol.2002.018713
[18]	Das S, Jayaratne R, Barrett K E. The role of ion transporters in the pathophysiology of infectious diarrhea[J]. Cell Mol Gastroenterol Hepatol, 2018, 6(1):33-45. doi: 10.1016/j.jcmgh.2018.02.009
[19]	Magalhaes D, Cabral J M, Soares-da-Silva P, et al. Role of epithelial ion transports in inflammatory bowel disease[J]. Am J Physiol Gastrointest Liver Physiol, 2016, 310(7):G460-G476. doi: 10.1152/ajpgi.00369.2015
[20]	Zhu C, Ye J L, Yang J, et al. Differential expression of intestinal ion transporters and water channel aquaporins in young piglets challenged with enterotoxigenic Escherichia coli K88[J]. J Anim Sci, 2017, 95(12):5240-5252. doi: 10.2527/jas2017.1806 pmid: 29293799
[21]	Zhi H, Yuan W T. Expression of aquaporin 3, 4, and 8 in colonic mucosa of rat models with slow transit constipation[J]. Zhonghua Wei Chang Wai Ke Za Zhi, 2011, 14(6):459-461.
[22]	Ishihara E, Nagahama M, Naruse S, et al. Neuropathological alteration of aquaporin 1 immunoreactive enteric neurons in the streptozotocin-induced diabetic rats[J]. Auton Neurosci, 2008, 138(1-2):31-40. doi: 10.1016/j.autneu.2007.09.002
[23]	Cao M, Yang M, Ou Z, et al. Involvement of aquaporins in a mouse model of rotavirus diarrhea[J]. Virol Sin, 2014, 29(4):211-217. doi: 10.1007/s12250-014-3469-z
[24]	Ikarashi N, Kon R, Sugiyama K. Aquaporins in the colon as a new therapeutic target in diarrhea and constipation[J]. Int J Mol Sci, 2016, 17(7):E1172.
[25]	Ikarashi N, Kon R, Iizasa T, et al. Inhibition of aquaporin-3 water channel in the colon induces diarrhea[J]. Biol Pharm Bull, 2012, 35(6):957-962. doi: 10.1248/bpb.35.957
[26]	Kon R, Yamamura M, Matsunaga Y, et al. Laxative effect of repeated Daiokanzoto is attributable to decrease in aquaporin-3 expression in the colon[J]. J Nat Med, 2018, 72(2):493-502. doi: 10.1007/s11418-018-1174-1
[27]	Cao Y, He Y, Wei C, et al. Aquaporins alteration profiles revealed different actions of senna, sennosides, and sennoside a in diarrhea-rats[J]. Int J Mol Sci, 2018, 19(10):E3210.
[28]	Guttman J A, Samji F N, Li Y, et al. Aquaporins contribute to diarrhoea caused by attaching and effacing bacterial pathogens[J]. Cell Microbiol, 2007, 9(1):131-141. pmid: 16889624
[29]	Hamabata T, Liu C, Takeda Y. Positive and negative regulation of water channel aquaporins in human small intestine by cholera toxin[J]. Microb Pathog, 2002, 32(6):273-277. doi: 10.1006/mpat.2002.0502
[30]	Yamamoto T, Kuramoto H, Kadowaki M. Downregulation in aquaporin 4 and aquaporin 8 expression of the colon associated with the induction of allergic diarrhea in a mouse model of food allergy[J]. Life Sci, 2007, 81(2):115-120. pmid: 17574630
[31]	Hardin J A, Wallace L E, Wong J F, et al. Aquaporin expression is downregulated in a murine model of colitis and in patients with ulcerative colitis, Crohn's disease and infectious colitis[J]. Cell Tissue Res, 2004, 318(2):313-323. pmid: 15338270
[32]	Ikarashi N. [The elucidation of the function and the expression control mechanism of aquaporin-3 in the colon][J]. [in Japanese]. Yakugaku Zasshi, 2013, 133(9):955-961. doi: 10.1248/yakushi.13-00173
[33]	Itoh A, Tsujikawa T, Fujiyama Y, et al. Enhancement of aquaporin-3 by vasoactive intestinal polypeptide in a human colonic epithelial cell line[J]. J Gastroenterol Hepatol, 2003, 18(2):203-210. doi: 10.1046/j.1440-1746.2003.02949.x
[34]	Cremon C, Carini G, Wang B, et al. Intestinal serotonin release, sensory neuron activation, and abdominal pain in irritable bowel syndrome[J]. Am J Gastroenterol, 2011, 106(7):1290-1298. doi: 10.1038/ajg.2011.86
[35]	Kon R, Ikarashi N, Hayakawa A, et al. Morphine-induced constipation develops with increased aquaporin-3 expression in the colon via increased serotonin secretion[J]. Toxicol Sci, 2015, 145(2):337-347. doi: 10.1093/toxsci/kfv055
[36]	Alcaino C, Knutson K R, Treichel A J, et al. A population of gut epithelial enterochromaffin cells is mechanosensitive and requires Piezo2 to convert force into serotonin release[J]. Proc Natl Acad Sci U S A, 2018, 115(32):E7632-E7641. doi: 10.1073/pnas.1804938115
[37]	Dimidi E, Christodoulides S, Scott S M, et al. Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation[J]. Adv Nutr, 2017, 8(3):484-494. doi: 10.3945/an.116.014407
[38]	Besecker E M, Deiter G M, Pironi N, et al. Mesenteric vascular dysregulation and intestinal inflammation accompanies experimental spinal cord injury[J]. Am J Physiol Regul Integr Comp Physiol, 2017, 312(1):R146-R156. doi: 10.1152/ajpregu.00347.2016
[39]	Bai C, An H, Wang S, et al. Treatment and prevention of bacterial translocation and endotoxemia with stimulation of the sacral nerve root in a rabbit model of spinal cord injury[J]. Spine (Phila Pa 1976), 2011, 36(5):363-371. doi: 10.1097/BRS.0b013e3181d25495
[40]	Frias B, Phillips A A, Squair J W, et al. Reduced colonic smooth muscle cholinergic responsiveness is associated with impaired bowel motility after chronic experimental high-level spinal cord injury[J]. Auton Neurosci, 2019, 216:33-38. doi: 10.1016/j.autneu.2018.08.005
[41]	Penning C, Delemarre J B, Bemelman W A, et al. Proximal and distal gut hormone secretion in slow transit constipation[J]. Eur J Clin Invest, 2000, 30(8):709-714. pmid: 10964163
[42]	Furness J B. The enteric nervous system and neurogastroenterology[J]. Nat Rev Gastroenterol Hepatol, 2012, 9(5):286-294. doi: 10.1038/nrgastro.2012.32 pmid: 22392290
[43]	Qualls-Creekmore E, Tong M, Holmes G M. Time-course of recovery of gastric emptying and motility in rats with experimental spinal cord injury [J]. Neurogastroenterol Motil, 2010, 22(1): 62-69, e27-e28.
[44]	Guo J, Zhu Y, Yang Y, et al. Electroacupuncture at Zusanli (ST36) ameliorates colonic neuronal nitric oxide synthase upregulation in rats with neurogenic bowel dysfunction following spinal cord injury[J]. Spinal Cord, 2016, 54(12):1139-1144. doi: 10.1038/sc.2016.76 pmid: 27377302

组别	n	术前	第1天	第3天	第7天	第14天	第28天	F值	P值
对照组	8	21.000±0.000	20.850±0.338	20.950±0.158	20.900±0.211	20.950±0.158	20.900±0.316	0.535	0.749
脊髓损伤组	8	21.000±0.000	0.000±0.000	0.100±0.211	4.100±0.738	7.100±0.460	9.700±0.350	4011.875	< 0.001
t值			195.373	250.200	69.230	90.135	75.132
P值			< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	术前	第3天	第14天	第28天	F值	P值
对照组	8	0.624±0.019	0.624±0.032	0.618±0.033	0.621±0.033	0.073	0.974
脊髓损伤组	8	0.621±0.018	0.523±0.023	0.538±0.016	0.547±0.015	44.840	< 0.001
t值		0.306	7.319	6.156	5.814
P值		0.764	< 0.001	< 0.001	< 0.001

组别	n	第3天	第14天	第28天	F值	P值
对照组	8	2.24±0.24	2.26±0.20	2.38±0.29	0.681	0.517
脊髓损伤组	8	5.44±0.77	5.85±0.88	5.53±0.53	0.688	0.514
t值		-11.263	-11.254	-14.823
P值		< 0.001	< 0.001	< 0.001

组别	n	第3天	第14天	第28天	F值	P值
对照组	8	2.39±0.16	2.31±0.18	2.37±0.14	0.518	0.603
脊髓损伤组	8	5.74±0.90	6.03±0.98	5.49±0.94	0.666	0.524
t值		-10.368	-10.569	-9.350
P值		< 0.001	< 0.001	< 0.001

组别	n	第3天	第14天	第28天	F值	P值
对照组	8	2.21±0.26	2.19±0.26	2.20±0.27	0.014	0.986
脊髓损伤组	8	5.14±0.16	5.40±0.13	5.25±0.92	0.085	0.919
t值		-5.165	-6.908	-9.041
P值		0.001	< 0.001	< 0.001

Aquaporin Over-expression for Constipation in Rats with Spinal Cord Injury

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 11

References 44

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LIU Dong, XU Zihan, LI Jiang, JU Ping. Effect of high-frequency repetitive transcranial magnetic stimulation in M1 region combined with dorsolateral prefrontal cortex on electroencephalogram θ frequency band amplitude of patients with neuropathic pain after spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2024, 30(1): 87-94.
[2]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[3]	LIU Ning, LIU Yuquan, ZHU Bin, YU Lingjia, TAN Haining, YANG Yong, LI Xiang. Application of International Standards for Neurological Classification of Spinal Cord Injury in China: a bibliometrics re-analysis [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 808-815.
[4]	WANG Yiji, ZHOU Hongjun, HE Zejia, LIU Genlin, ZHENG Ying, HAO Chunxia, WEI Bo, KANG Haiqiong, ZHANG Ying, LU Xiaolei, YUAN Yuan, MENG Qianru. Relationship between symmetry of lower limb function and gait symmetry in patients with incomplete spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(6): 639-645.
[5]	YUAN Yuan, ZHOU Hongjun, CONG Xinying, LIU Genlin, WEI Bo, ZHENG Ying, HAO Chunxia, ZHANG Ying, WANG Yiji, KANG Haiqiong, LU Xiaolei, MENG Qianru. Relationship between impairment and magnetic resonance imaging finding in patients with traumatic cervical spinal cord injury after surgery [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(6): 725-730.
[6]	JIANG Le, DU Liangjie, HUANG Fubiao. Mood states and cognitive performance in patients with complete spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 576-581.
[7]	GUO Shuang, XIE Yongqi, ZHANG Liang, ZHANG Chunjia, PENG Run, YANG Degang, YANG Mingliang. Related factors and prediction model for neurological outcome of dance-associated pediatric spinal cord injury without radiographic abnormality [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(5): 582-589.
[8]	ZHANG Yuan, YANG Jian. Exercise rehabilitation interventions for people with spinal cord injury and their health benefits: a systematic review of systematic reviews based on ICD-11 and ICF [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(12): 1377-1385.
[9]	SHI Xiaoyu, YANG Jian. Adaptive physical activity and its health benefits for patients with spinal cord injury based on ICF: a scoping review [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(12): 1395-1404.
[10]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[11]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[12]	LIU Genlin,ZHOU Hongjun,LI Jianjun,WEI Bo,ZHENG Ying,HAO Chunxia,ZHANG Ying,WANG Yiji,KANG Haiqiong,LU Xiaolei,YUAN Yuan,MENG Qianru. Advance in neurological classification of spinal cord injury with complications [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(8): 934-938.
[13]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.
[14]	KANG Haiqiong,ZHOU Hongjun,LIU Genlin,WEI Bo,ZHENG Ying,ZHANG Ying,HAO Chunxia,WANG Yiji,LU Xiaolei,YUAN Yuan,MENG Qianru. Changes of bone mineral density in distal femur and proximal tibia in patients with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 855-858.
[15]	ZHANG Miaoyuan,HE Ying,LI Xiaoxia,PENG Min,ZHANG Lei,LIU Shuying,KONG Ying. Self-management status and related factors of patients with intermittent clean catheterization after spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(6): 716-724.