丰富环境对缺血性脑卒中小鼠海马区Bcl-2和Bax蛋白表达和认知功能的效果

doi:10.3969/j.issn.1006-9771.2020.05.009

Abstract

Abstract:

Objective To explore the effect of enriched environment on spatial learning and memory of mice after ischemic stroke and the expression of apoptotic regulatory protein Bcl-2 and Bax in hippocampus.Methods Clean grade adult male C57BL/6 mice (n = 42) were occluded left middle cerebral artery permanently. Three days after operation, the modeling mice were randomly divided into standard group (n = 16) and enriched environment group (n = 16). Other eight mice were as sham group. The sham group and the standard group were fed in standard environment, while the enriched group was fed in enriched environment. They were tested with Morris Water Maze 21 days after intervention, and the expression of Bcl-2 and Bax protein in hippocampus was detected with Western blotting and immunofluorescence staining.Results The latency was less in the enriched group than in the standard group (P < 0.05), and the time and swimming distance staying in the target quadrant were more ( P < 0.05), as well as the times crossing the target quadrant ( P < 0.05). Compared with the standard group, the expression of Bcl-2 increased in the enriched group, while the expression of Bax decreased ( P < 0.05). Conclusion Enriched environment can improve the spatial learning and memory of mice after ischemic stroke, which may be associated with reducing apoptosis in hippocampus.

Key words: ischemic stroke, enriched environment, cognition, apoptosis, mice

CLC Number:

R743.32

WANG Chuan-jie,WU Yi,TAO Feng,YANG Lei. Effects of Enriched Environment on Cognition and Expression of Bcl-2/Bax Protein in Hippocampus after Ischemic Stroke in Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(5): 539-543.

Figures/Tables 6

References 35

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组别	n	第1天	第2天	第3天	第4天	第5天	F值	P值
假手术组	8	63.8±16.5	51.9±13.8	43.3±12.5	30.3±13.8	20.6±9.2	17.328	< 0.001
标准饲养组	8	62.4±13.3	56.2±16.8^a	52.7±14.8^a	46.9±12.6^a	40.4±10.8^a	4.765	0.013
丰富环境组	8	60.2±11.8	53.5±10.9^b	46.5±15.6^b	32.1±9.8^b	22.9±7.3^b	15.863	< 0.001
F值		0.308	3.343	6.342	10.239	11.298
P值		0.792	0.028	0.011	< 0.001	< 0.001

组别	n	游动距离(m)	游动时间(s)	穿过原平台次数
假手术组	8	6.3±2.5	21.9±3.6	3.9±0.5
标准饲养组	8	2.4±1.3^a	8.4±2.9^a	1.2±0.6^a
丰富环境组	8	5.8±1.8^b	19.5±2.3^b	3.5±0.3^b
F值		12.728	13.146	7.932
P值		< 0.001	< 0.001	0.002

组别	n	Bcl-2	Bax	Bcl-2/Bax
假手术组	8	0.893±0.125	0.497±0.145	1.753±0.138
标准饲养组	8	0.497±0.139^a	0.804±0.119^a	0.671±0.163^a
丰富环境组	8	0.943±0.128^b	0.625±0.133^b	1.435±0.103^b
F值		13.384	12.751	15.654
P值		< 0.001	< 0.001	< 0.001

组别	n	Bcl-2	Bax	Bcl-2/Bax
假手术组	8	10976.8±0.125	4064.7±527.5	2.644±0.168
标准饲养组	8	4896.9±0.139^a	8065.8±709.9^a	0.609±0.119^a
丰富环境组	8	9825.3±886.1^b	5389.5±587.3^b	1.805±0.171^b
F值		17.769	14.264	19.096
P值		< 0.001	< 0.001	< 0.001

Effects of Enriched Environment on Cognition and Expression of Bcl-2/Bax Protein in Hippocampus after Ischemic Stroke in Mice

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