任务导向性康复训练对小鼠脊髓损伤后神经回路可塑性及前肢运动功能的影响

doi:10.3969/j.issn.1006-9771.2020.10.006

Abstract

Abstract:

Objective To explore the effect of task-based rehabilitative training on neural circuit plasticity and forelimb motor function after C₅ spinal cord injury in mice.
Methods A total of 21 healthy C57/BL mice were randomly and equally divided into sham group, model group and training group. The model was established by left C₅ spinal cord crush injury. The lamina was removed without damaging the spinal cord in the sham group. Four weeks after injury, the training group received task-based rehabilitative training for four weeks. The horizontal ladder and rearing tests were used to assess motor function for forelimb before injury, and three days, two weeks, four weeks, six weeks and eight weeks after injury. The axons of the corticospinal tract in all mice were observed six weeks after injury by using biotinylated dextran amin (BDA) anterograde tracing. Eight weeks after injury, motor-evoked potential was applied to measure nerve conduction velocities in forelimb, while the axon sprouting and syntagmatic relation of neuron in the anterior horn of gray matter above lesion were observed by immunofluorescence double-labeling of BDA/neuron-specific nuclei protein (NeuN); the expression of Synapsin in the anterior horn of gray matter at lesion was observed by immunofluorescence double-labeling of NeuN/Synapsin I.
Results Eight weeks after injury, the latency of P1 and N1 was longer in the model group than in the sham group ( P < 0.05), and was shorter in the training group than in the model group ( P < 0.05). Compared with the sham group, the error rate of left forelimb increased, and the usage rate decreased ( P < 0.05) in the model group and the training group; compared with the model group, the error rate of left forelimb decreased six weeks and eight weeks after injury ( P < 0.05), and the usage rate increased eight weeks after injury ( P < 0.05) in the treatment group. Compared with the model group, more axon sprouting co-localized with neurons in the anterior horn of gray matter above lesion ( P < 0.05), and the expression of Synapsin I increased in the training group ( P < 0.05).
Conclusion Task-based rehabilitative training could promote the neural circuit plasticity and improve the motor function of forelimb after spinal cord injury in mice.

Key words: spinal cord injury, task-based rehabilitative training, neuroplasticity, forelimb, motor function, mice

CLC Number:

R651.2

PAN Lu,TAN Bo-tao,LUO Mei-ling,LIU Yuan,WU Ya-min,YU Le-hua,YIN Ying. Effect of Task-based Rehabilitation Training on Neural Circuit Plasticity and Forelimb Motor Function post Spinal Cord Injury in Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(10): 1152-1160.

Figures/Tables 10

References 33

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组别	n	P1	N1
假手术组	7	5.300±0.265	6.343±0.658
模型组	7	7.986±1.404^a	9.386±1.826^a
训练组	7	5.886±0.471^b	6.943±0.820^b
F值		18.508	12.285
P值		< 0.001	< 0.001

组别	n	术前	术后3 d	术后2周	术后4周	术后6周	术后8周
假手术组	7	1.7±1.0	1.1±0.9	1.6±0.9	1.3±1.5	1.6±1.6	1.1±0.5
模型组	7	2.2±1.7	31.8±6.3^a	16.5±4.8^a	12.8±4.0^a	22.3±9.3^a	15.2±2.7^a
训练组	7	2.1±1.6	27.4±3.1^a	18.1±2.3^a	10.3±5.1^a	11.8±3.6^a,b	8.8±2.9^a,b
F值		0.355	115.523	60.251	17.240	22.227	65.328
P值		0.706	< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	术前	术后3 d	术后2 周	术后4周	术后6周	术后8周
假手术组	7	8.6±6.9	10.0±5.8	12.1±3.9	12.8±4.9	10.8±8.4	10.8±4.5
模型组	7	11.4±6.9	1.4±3.8^a	3.6±6.2^a	5.0±5.0^a	4.3±4.5	3.6±4.8^a
训练组	7	10.0±7.1	2.9±4.9^a	2.9±4.9^a	4.1±5.3^a	8.6±6.9	14.3±5.3^b
F值		0.265	6.200	7.136	6.138	1.629	8.750
P值		0.770	0.009	0.005	0.009	0.224	0.002

组别	n	轴突(a.u. /mm²)	NeuN/BDA(个/mm²)
假手术组	7	267325.286±97575.678	17.143±4.451
模型组	7	768600.857±86511.326	19.143±4.811
训练组	7	1861362.762±771881.369^a,b	33.286±4.990^a,b
F值		32.019	23.964
P值		< 0.001	< 0.001

组别	n	Synapsin I(a.u. /mm²)
假手术组	7	35.545±2.903
模型组	7	15.760±1.469^a
训练组	7	25.080±1.812^a,b
F值		148.354
P值		< 0.001

Effect of Task-based Rehabilitation Training on Neural Circuit Plasticity and Forelimb Motor Function post Spinal Cord Injury in Mice

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