穴位埋线对局灶性脑缺血再灌注损伤大鼠运动功能的影响

doi:10.3969/j.issn.1006-9771.2020.10.008

Abstract

Abstract:

Objectives To investigate the effect of acupoint catgut embedding on motor function of rats with cerebral ischemia-reperfusion, and explore its possible mechanism.
Methods A total of 48 three-month old healthy specified pathogen free male Sprague-Dawley rats were randomly divided into three groups with 16 rats in each group. The right external carotid artery was severed only in sham operation group. Right middle cerebral artery occlusion was induced for 90 minutes under isoflurane anesthesia using a monofilament in model group and acupoint catgut embedding group. Ninety minutes after reperfusion, the acupoint catgut embedding group accepted acupoint catgut embedding on Baihui (DU20) acupoint and anterior oblique line of vertex-temporal. After one, three, seven and 14 days of reperfusion, they were assessed with Neurological Evaluation and Screen Prehensile Test. After 14 days of reperfusion, the infarct volume was measured with TTC staining; the expression of glial fibrillary acidic protein (GFAP) and Synapsin I was detected with immunohistochemistry, Western blotting and reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR).
Results Compared with the sham operation group, the neurological score significantly decreased (t > 11.851, P < 0.001), the prehensile time significantly decreased ( t > 6.190, P < 0.001), the expression of GFAP increased ( P < 0.05), and the expression of Synapsin I decreased ( P < 0.05) in the model group. Compared with the model group, the neurological score significantly increased ( t > 3.464, P < 0.001), the prehensile time significantly increased ( t > 3.642, P < 0.001), the cerebral infarction volume significantly decreased ( F = 93.426, P < 0.001), the expression of GFAP decreased ( P < 0.05), and the expression of Synapsin I increased ( P < 0.05) in the acupoint catgut embedding group.
Conclusion Acupoint catgut embedding could promote the recovery of motor function of rats with cerebral ischemia-reperfusion injury, which may be related with inhibiting the proliferation of astrocytes, increasing the regeneration of axons in the ischemic penumbra and enhancing synaptic connection.

Key words: ischemia-reperfusion injury, acupoint catgut embedding, motor function, synaptic plasticity, rats

CLC Number:

R743.3

HUANG Qin,ZHOU Li-zhi,TAN Cai-ling,ZHENG Su,PENG Li. Effects of Acupoint Catgut Embedding on Motor Function in Cerebral Ischemia-reperfusion Injury in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(10): 1167-1175.

Figures/Tables 10

References 42

[1]	GBD 2013 Mortality and Causes of Death Collaborators. Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013BD 2013 Mortality and Causes of Death Collaborators. Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013[J]. Lancet, 2015, 385(9963):117-171. doi: 10.1016/S0140-6736(14)61682-2
[2]	Yeo L L, Paliwal P, Teoh H L, et al. Timing of recanalization after intravenous thrombolysis and functional outcomes after acute ischemic stroke[J]. JAMA Neurol, 2013, 70(3):353-358. doi: 10.1001/2013.jamaneurol.547
[3]	Biondi O, Grondard C, Lecolle S, et al. Exercise-induced activation of NMDA receptor promotes motor unit development and survival in a type 2 spinal muscular atrophy model mouse[J]. J Neurosci, 2008, 28(4):953-962. doi: 10.1523/JNEUROSCI.3237-07.2008
[4]	Huo J, Zhao J, Yuan Y, et al. [Research status of the effect mechanism on catgut-point embedding therapy][J]. [in Chinese]. Zhongguo Zhen Jiu, 2017, 37(11):1251-1254.
[5]	Guo A S, Li A H, Chen X, et al. [Effect of acupoint catgut embedding on motor function and serum high sensitivity C-reactive protein and IL-6 levels in patients with acute cerebral infarction][J]. [in Chinese]. Zhen Ci Yan Jiu, 2013, 38(3):224-228, 258.
[6]	Longa E Z, Weinstein P R, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1):84-91. pmid: 2643202
[7]	Garcia J H, Wagner S, Liu K F, et al. Neurological deficit and extent of neuronal necrosis attributable to middle cerebral artery occlusion in rats. Statistical validation[J]. Stroke, 1995, 26(4):627-634, 635. pmid: 7709410
[8]	Ashwal S, Tone B, Tian H R, et al. Core and penumbral nitric oxide synthase activity during cerebral ischemia and reperfusion[J]. Stroke, 1998, 29(5):1037-1046, discussion 1047. pmid: 9596255
[9]	Cervera M A, Soekadar S R, Ushiba J, et al. Brain-computer interfaces for post-stroke motor rehabilitation: a meta-analysis[J]. Ann Clin Transl Neurol, 2018, 5(5):651-663. doi: 10.1002/acn3.2018.5.issue-5
[10]	Biasiucci A, Leeb R, Iturrate I, et al. Brain-actuated functional electrical stimulation elicits lasting arm motor recovery after stroke[J]. Nat Commun, 2018, 9(1):2421. doi: 10.1038/s41467-018-04673-z pmid: 29925890
[11]	Bockbrader M A, Francisco G, Lee R, et al. Brain computer interfaces in rehabilitation medicine[J]. PM R, 2018, 10:233-243. doi: 10.1016/S1934-1482(18)30028-5
[12]	Mehrholz J, Pohl M, Platz T, et al. Electromechanical and robot-assisted arm training for improving activities of daily living, arm function, and arm muscle strength after stroke[J]. Cochrane Database Syst Rev, 2018, 9:D6876.
[13]	Chisari C, Bertolucci F, Monaco V, et al. Robot-assisted gait training improves motor performances and modifies Motor Unit firing in poststroke patients[J]. Eur J Phys Rehabil Med, 2015, 51(1):59-69.
[14]	Baker E W, Kinder H A, West F D. Neural stem cell therapy for stroke: a multimechanistic approach to restoring neurological function[J]. Brain Behav, 2019, 9(3):e1214.
[15]	Huang L, Zhang L. Neural stem cell therapies and hypoxic-ischemic brain injury[J]. Prog Neurobiol, 2019, 173:1-17. doi: S0301-0082(17)30113-2 pmid: 29758244
[16]	Webb R L, Kaiser E E, Scoville S L, et al. Human neural stem cell extracellular vesicles improve tissue and functional recovery in the murine thromboembolic stroke model[J]. Transl Stroke Res, 2018, 9(5):530-539. doi: 10.1007/s12975-017-0599-2
[17]	许兰, 李奕琴, 徐远红. 穴位埋线对脑卒中后运动功能障碍及生活质量的影响[J]. 世界最新医学信息文摘, 2017, 17(90):93-98.
	Xu L, Li Y Q, Xu Y H, et al. Effects of acupoint catgut embedding on motor dysfunction and quality of life after stroke[J]. World Latest Med Inform, 2017, 17(90):93-98.
[18]	Sharp F R, Lu A, Tang Y, et al. Multiple molecular penumbras after focal cerebral ischemia[J]. J Cereb Blood Flow Metab, 2000, 20(7):1011-1032.
[19]	Chen Z, Hu Q, Xie Q, et al. Effects of treadmill exercise on motor and cognitive function recovery of MCAO mice through the caveolin-1/VEGF signaling pathway in ischemic penumbra[J]. Neurochem Res, 2019, 44(4):930-946. doi: 10.1007/s11064-019-02728-1
[20]	Thirugnanachandran T, Ma H, Singhal S, et al. Refining the ischemic penumbra with topography[J]. Int J Stroke, 2018, 13(3):277-284. doi: 10.1177/1747493017743056 pmid: 29140184
[21]	Canedo A. Primary motor cortex influences on the descending and ascending systems[J]. Prog Neurobiol, 1997, 51(3):287-335. pmid: 9089791
[22]	Rouiller E M, Olivier E. Functional recovery after lesions of the primary motor cortex[J]. Prog Brain Res, 2004, 143:467-475. pmid: 14653189
[23]	Wang X, Chen A, Wu H, et al. Enriched environment improves post-stroke cognitive impairment in mice by potential regulation of acetylation homeostasis in cholinergic circuits[J]. Brain Res, 2016, 1650:232-242. doi: S0006-8993(16)30646-1 pmid: 27637156
[24]	Nie J, Yang X. Modulation of synaptic plasticity by exercise training as a basis for ischemic stroke rehabilitation[J]. Cell Mol Neurobiol, 2017, 37(1):5-16. doi: 10.1007/s10571-016-0348-1
[25]	Peters D M, Fridriksson J, Stewart J C, et al. Cortical disconnection of the ipsilesional primary motor cortex is associated with gait speed and upper extremity motor impairment in chronic left hemispheric stroke[J]. Hum Brain Mapp, 2018, 39(1):120-132. doi: 10.1002/hbm.23829
[26]	Kang N, Weingart A, Cauraugh J H. Transcranial direct current stimulation and suppression of contralesional primary motor cortex post-stroke: a systematic review and meta-analysis[J]. Brain Inj, 2018, 32(9):1063-1070. doi: 10.1080/02699052.2018.1481526
[27]	Thompson S N, Gibson T R, Thompson B M, et al. Relationship of calpain-mediated proteolysis to the expression of axonal and synaptic plasticity markers following traumatic brain injury in mice[J]. Exp Neurol, 2006, 201(1):253-265. doi: 10.1016/j.expneurol.2006.04.013
[28]	Eng L F, Ghirnikar R S, Lee Y L. Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000)[J]. Neurochem Res, 2000, 25(9-10):1439-1451. pmid: 11059815
[29]	Shen S W, Duan C L, Chen X H, et al. Neurogenic effect of VEGF is related to increase of astrocytes transdifferentiation into new mature neurons in rat brains after stroke[J]. Neuropharmacology, 2016, 108:451-461. doi: 10.1016/j.neuropharm.2015.11.012
[30]	Yang Z, Wang K K. Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker[J]. Trends Neurosci, 2015, 38(6):364-374. doi: 10.1016/j.tins.2015.04.003
[31]	宋晓磊, 冯晓东. 穴位埋线治疗脑卒中后肌张力障碍临床研究[J]. 中医学报, 2011, 26(12):1533-1534.
	Song X L, Feng X D. Clinical research on catgut embedding therapy in treating dystonia after stroke[J]. Chin J Chin Med, 2011, 26(12):1533-1534.
[32]	全超, 徐远红. 穴位埋线对脑卒中偏瘫患者步行功能影响的三维步态研究[J]. 世界最新医学信息文摘, 2019, 19(20):158-159.
	Quan C, Xu Y H. The clinical effect of acupoint embedding therapy on hemiplegic gait based on evaluating by gait analysis[J]. World Latest Med Inform, 2019, 19(20):158-159.
[33]	冯晓东, 李瑞青, 任彬彬, 等. 穴位埋线对脑卒中后上肢痉挛患者的疗效观察[J]. 中国康复医学杂志, 2013, 28(9):843-845.
	Feng X D, Li R Q, Ren B B, et al. The clinical effect of acupoint embedding therapy on patients with upper limb spasm after stroke[J]. Chin J Rehabil Med, 2013, 28(9):843-845.
[34]	余智. 病灶头皮反射区埋线治疗脑卒中后感觉障碍临床研究[J]. 中国针灸, 2014, 34(7):631-635.
	Yu Z. Clinical research of catgut implantation at scalp reflecting foci for post-stroke sensory disturbance[J]. Chin Acupunc Moxib, 2014, 34(7):631-635.
[35]	李瑞青, 徐志伟, 孙伟娟, 等. 穴位埋线对脑缺血再灌注后肢体痉挛大鼠谷氨酰胺合成酶表达的影响[J]. 中华中医药杂志, 2018, 33(10):4736-4739.
	Li R Q, Xu Z W, Sun W J, et al. Effects of catgut implantation in acupoints on GS expressions in brain of rats with spasticity after stroke[J]. Zhong Hua Zhong Yi Yao Za Zhi, 2018, 33(10):4736-4739.
[36]	Huang F, Xie Y, Zhao S, et al. The effectiveness and safety of acupoint catgut embedding for the treatment of postmenopausal osteoporosis: a systematic review and meta-analysis[J]. Evid Based Complement Alternat Med, 2019, 2019:2673763.
[37]	Zhang L, Wang Y, Xu Y, et al. Metabonomic analysis reveals efficient ameliorating effects of acupoint stimulations on the menopause-caused alterations in mammalian metabolism[J]. Sci Rep, 2014, 4:3641. doi: 10.1038/srep03641
[38]	Chen G Z, Xu Y X, Zhang J W, et al. Effect of acupoint catgut-embedding on the quality of life, reproductive endocrine and bone metabolism of postmenopausal women[J]. Chin J Integr Med, 2010, 16(6):498-503. doi: 10.1007/s11655-010-0574-1
[39]	Du K, Wang X, Chi L, et al. Role of sigma-1 receptor/p38 MAPK inhibition in acupoint catgut embedding-mediated analgesic effects in complete freund's adjuvant-induced inflammatory pain[J]. Anesth Analg, 2017, 125(2):662-669. doi: 10.1213/ANE.0000000000001857
[40]	Cui W Q, Sun W S, Xu F, et al. Spinal serotonin 1A receptor contributes to the analgesia of acupoint catgut embedding by inhibiting phosphorylation of the N-methyl-D-aspartate receptor GluN1 subunit in complete Freund's adjuvant-induced inflammatory pain in rats[J]. J Pain, 2019, 20(1):11-16.
[41]	李箭, 李义, 马福彦. 穴位埋线疗法在髋关节置换术后镇痛的疗效[J]. 长春中医药大学学报, 2017, 33(5):781-783.
	Li J, Li Y, Ma F Y, et al. Acesodyne effect of catgut embedment in acupoint therapy after hip replacemen[J]. J Changchun Univ Chin Med, 2017, 33(5):781-783.
[42]	陈盼碧, 陈静, 崔瑾, 等. 穴位埋线法对原发性痛经大鼠神经-内分泌-免疫网络的调控与影响[J]. 针刺研究, 2018, 43(1):29-33.
	Chen P B, Chen J, Cui J, et al. Effects of the acupoint catgut embedding on nerve-endocrine-immune network in dysmenorrhea rat[J]. Zhen Ci Yan Jiu, 2018, 43(1):29-33.

组别	n	第1天	第3天	第7天	第14天	F值	P值
假手术组	8	18.000±0.000	18.000±0.000	18.000±0.000	18.000±0.000
模型组	8	9.714±0.680	10.429±0.649	11.143±0.404	12.143±0.349	4.849	0.012
穴位埋线组	8	12.000±0.436	13.000±0.436	14.429±0.481	15.857±0.459	34.972	0.001
F值		84.125	72.633	89.431	80.688
P值		< 0.001	< 0.001	< 0.001	< 0.001
t值^a		12.557	11.851	13.370	12.554
P值^a		< 0.001	< 0.001	< 0.001	< 0.001
t值^b		3.464	4.025	6.406	7.961
P值^b		< 0.001	< 0.001	< 0.001	< 0.001

组别	n	第1天	第3天	第7天	第14天	F值	P值
假手术组	6	56.633±1.570	57.682±1.314	60.000±0.000	60.000±0.000	3.632	0.086
模型组	6	16.248±4.453	20.390±4.613	28.148±3.685	38.028±3.035	28.255	0.002
穴位埋线组	6	41.593±5.143	46.118±4.128	46.920±4.309	53.307±3.113	4.979	0.014
F值		25.636	27.297	23.919	20.132
P值		< 0.001	< 0.001	< 0.001	< 0.001
t值^a		7.084	7.217	6.880	6.190
P值^a		< 0.001	< 0.001	< 0.001	< 0.001
t值^b		4.446	4.979	4.055	4.304
P值^b		< 0.001	< 0.001	< 0.001	< 0.001

组别	n	第1天	第3天	第7天	第14天	F值	P值
假手术组	6	57.905±1.290	58.090±1.744	59.233±0.767	56.518±1.846	0.880	0.474
模型组	6	5.002±0.503	9.040±0.744	12.058±0.557	21.612±1.599	131.541	< 0.001
穴位埋线组	6	11.565±1.719	22.003±1.785	30.260±1.810	37.283±2.027	33.492	< 0.001
F值		511.961	285.814	406.954	91.017
P值		< 0.001	< 0.001	< 0.001	< 0.001
t值^a		29.352	23.070	28.284	13.469
P值^a		< 0.001	< 0.001	< 0.001	< 0.001
t值^b		3.642	6.097	10.913	6.047
P值^b		< 0.001	< 0.001	< 0.001	< 0.001

组别	n	脑梗死体积
假手术组	4	0
模型组	4	22.7±1.899
穴位埋线组	4	5.64±0.9384^a

组别	n	GFAP	突触素I
假手术组	4	0.046±0.008	0.081±0.011
模型组	4	0.061±0.007^a	0.043±0.010^a
穴位埋线组	4	0.035±0.008^b	0.059±0.007^b
F值		4.060	4.230
P值		< 0.05	< 0.05

Effects of Acupoint Catgut Embedding on Motor Function in Cerebral Ischemia-reperfusion Injury in Rats

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组别	n	GFAP蛋白	突触素I蛋白
假手术组	4	2.146±0.597	1.348±0.218
模型组	4	3.049±1.223^a	0.515±0.426^a
穴位埋线组	4	1.930±1.177^b	1.156±0.262^b
F值		0.326	1.917
P值		< 0.05	< 0.05

组别	n	GFAP mRNA	突触素I mRNA
假手术组	4	1.439±0.184	2.690±0.096
模型组	4	0.942±0.015^a	0.917±0.065^a
穴位埋线组	4	0.457±0.063^b	1.447±0.069^b
F值		19.014	135.629
P值		< 0.05	< 0.05

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