推拿对失神经肌萎缩大鼠肌特异性microRNA和肌卫星细胞增殖分化相关因子的影响

doi:10.3969/j.issn.1006-9771.2020.11.009

Abstract

Abstract:

Objective To investigate the effects and mechanism of Tuina on denervation-induced atrophy.Methods A total of 42 Sprague-Dawley rats were randomly divided into sham group (n = 6), model group (n = 18) and Tuina group (n = 18). The model group and Tuina group freed and excised right tibia nerve about one centimeter, while the sham group freed the right tibia nerve only. From the second day after operation, Tuina group accepted Tuina on the injured area, while the sham group and the model group were only fixed without any intervention. Six rats were sacrificed on the 14th, 21st and 28th day after operation in the model and Tuina groups, and the sham group was sacrificed on the 28th day after operation. The gastrocnemius muscles were measured wet weight ratio. The diameter and area of muscle cells were measured under HE staining. The expression of Pax7, MyoD, MyoG, microRNA-1, microRNA-133a and microRNA-206 in the gastrocnemius muscles were detected with reverse transcription real-time quantitative polymerase chain reaction.Results Compared with the sham group, the wet weight ratio, the area of muscle cells (except the 14-day-Tuina group) and the diameter of muscle cells decreased at each time point in the model group and Tuina group (P < 0.05); compared with the model group, the wet weight ratio, muscle cell diameter and muscle cell area increased at each time point in Tuina group ( P < 0.05). Compared with the sham group, the expression of Pax7 increased in the 14-day-model group ( P < 0.05) and decreased in the 28-day-model group ( P < 0.05), and it increased at each time point (except 28-day) in Tuina group ( P < 0.05); compared with the model group, the expression of Pax7 increased at each time point in Tuina group ( P < 0.05). Compared with the sham group, the expression of MyoD and MyoG increased at each time point in the model group and Tuina group ( P < 0.05); compared with the model group, the expression of MyoD and MyoG increased at each time point (except 14-day) in Tuina group ( P < 0.05). Compared with the sham group, the expression of microRNA-1 and microRNA-133a decreased, and microRNA-206 increased in the model group and Tuina group at 21-day ( P < 0.05); compared with the model group, the expression of microRNA-1, microRNA-133a and microRNA-206 increased in Tuina group ( P < 0.05). Conclusion Tuina may activate the Pax7/MyoD/MyoG pathway by increasing the expression of muscle-specific microRNA, to promote the proliferation and differentiation of muscle satellite cells, and delay denervation-induced atrophy.

Key words: denervation-induced atrophy, Tuina, muscle satellite cells, proliferation, differentiation, microRNA, rats

CLC Number:

R685

MA Xiang,TANG Cheng-lin,ZHAO Dan-dan,AN Hui-yu,WAN Xiao-feng,QIAO Tong-qian. Effects of Tuina on Muscle-specific microRNA and Factors Related with Proliferation and Differentiation of Muscle Satellite Cells in Denervation-induced Atrophy in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(11): 1297-1304.

Figures/Tables 8

References 33

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组别	n	14 d	21 d	28 d
假手术组	6	1.00±0.02^*	1.00±0.02^*	1.00±0.02
模型组	6	0.58±0.07^a	0.38±0.03^a	0.25±0.02^a
推拿组	6	0.70±0.04^a,b	0.46±0.04^a,b	0.37±0.06^a,b
F值		115.220	865.980	677.010
P值		< 0.001	< 0.001	< 0.001

组别	n	14 d	21 d	28 d
假手术组	6	39.92±0.69^*	39.92±0.69^*	39.92±0.69
模型组	6	34.14±1.04^a	30.83±1.81^a	23.85±1.97^a
推拿组	6	38.11±1.33^a,b	32.86±0.96^a,b	26.93±0.71^a,b
F值		46.909	87.552	270.374
P值		< 0.001	< 0.001	< 0.001

组别	n	14 d	21 d	28 d
假手术组	6	1028.27±19.33^*	1028.27±19.33^*	1028.27±19.33
模型组	6	815.56±102.71^a	733.10±66.23^a	572.43±54.44^a
推拿组	6	967.50±79.14^b	868.17±15.67^a,b	653.08±37.16^a,b
F值		12.277	76.807	222.430
P值		0.001	< 0.001	< 0.001

组别	n	14 d	21 d	28 d
假手术组	6	1.01±0.12^*	1.01±0.12^*	1.01±0.12
模型组	6	1.83±0.15^a	1.06±0.44	0.50±0.21^a
推拿组	6	2.49±0.33^a,b	1.67±0.34^a,b	1.09±0.40^b
F值		67.533	7.651	8.293
P值		< 0.001	0.005	0.004

组别	n	14 d	21 d	28 d
假手术组	6	1.09±0.16^*	1.09±0.16^*	1.09±0.16
模型组	6	3.96±0.53^a	8.65±0.44^a	6.29±0.53^a
推拿组	6	4.65±0.34^a,b	11.63±0.50^a,b	7.49±0.29^a,b
F值		154.470	1132.584	533.664
P值		< 0.001	< 0.001	< 0.001

Effects of Tuina on Muscle-specific microRNA and Factors Related with Proliferation and Differentiation of Muscle Satellite Cells in Denervation-induced Atrophy in Rats

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组别	n	14 d	21 d	28 d
假手术组	6	1.01±0.15^*	1.01±0.15^*	1.01±0.15
模型组	6	21.65±1.49^a	33.73±1.48^a	13.36±0.77^a
推拿组	6	21.64±0.91^a	48.04±2.00^a,b	32.84±2.77^a,b
F值		833.071	1688.995	560.949
P值		< 0.001	< 0.001	< 0.001

组别	n	microRNA-133a	microRNA-206	microRNA-1
假手术组	6	1.03±0.18^*	1.05±0.31^*	1.01±0.13^*
模型组	6	0.21±0.06^a	3.11±0.51^a	0.59±0.11^a
推拿组	6	0.46±0.13^a,b	4.94±1.11^a,b	0.77±0.07^a,b
F值		57.763	42.764	23.095
P值		< 0.001	< 0.001	< 0.001

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