减重平板训练对脊髓损伤大鼠神经病理性疼痛及脊髓后角谷氨酸脱羧酶-65/67表达的影响

doi:10.3969/j.issn.1006-9771.2021.02.002

Abstract

Abstract: Objective

To investigate the effect of body weight-supported treadmill training on neuropathic pain and expression of glutamate decarboxylase-65/67 (GAD-65/67) in spinal dorsal horn of rats with spinal cord injury (SCI).

Methods

A total of 24 Sprague-Dawley rats were randomly divided into sham group, SCI-sedentary (SCI-Sed) group and SCI-Exercise (SCI-Ex) group, with eight rats in each group. Allen's method was used to make T₁₀ incomplete SCI model. Seven days after SCI, SCI-Ex group was given body weight-supported treadmill training. Before SCI, and seven days, 14 days, 21 days, 28 days and 35 days after SCI, the von Frey filaments and thermal stimulation pain tester were used to evaluate the mechanical and thermal pain thresholds. Then, Western blotting and immunohistochemical analysis were performed on the spinal cord of all rats to detect the expression of GAD-65 and GAD-67.

Results

The mechanical and thermal pain thresholds were higher in SCI-Ex group than in SCI-Sed group 21 days, 28 days and 35 days after SCI (P < 0.01). Compared with the sham group, the expression of GAD-65 and GAD-67 decreased in SCI and SCI-Ex groups (P < 0.05), and increased in SCI-Ex group compared with that of SCI-Sed group (P < 0.05).

Conclusion

Body weight-supported treadmill training could increase the synthesis of GAD-65/67 in the distal spinal cord dorsal horn of incomplete SCI rats, and improve the pain thresholds of hind limbs in rats with SCI.

Key words: spinal cord injury, body weight-supported treadmill training, neuropathic pain, γ-aminobutyric acid, glutamate decarboxylase-65/67, rats

CLC Number:

R651.2

Xiang-zhe LI,Jie DING,Qing-hua WANG,Chuan-ming DONG,Tong WANG,Qin-feng WU. Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase (GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 131-136.

Figures/Tables 6

References 29

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组别	n	术前	7 d	14 d	21 d	28 d	35 d
Sham组	8	6.50±1.41	6.50±1.41	6.75±1.49	6.25±1.28	6.75±1.49	6.50±0.93
SCI-Sed组	8	6.25±1.67	2.05±1.91^a	0.70±0.26^a	0.50±0.11^a	0.63±0.25^a	0.70±0.26^a
SCI-Ex组	8	6.25±1.67	1.95±1.97^a	1.93±1.72^a	2.05±0.87^a,b	2.93±1.17^a,c	3.50±1.41^a,c
F值		0.066	16.952	46.941	87.881	63.191	69.016
P值		0.936	< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	术前	7 d	14 d	21 d	28 d	35 d
Sham组	8	15.45±3.55	15.31±5.50	14.58±2.87	15.55±2.35	15.74±2.77	16.92±1.63
SCI-Sed组	8	15.72±4.47	3.55±2.46^a	4.05±2.98^a	3.47±2.20^a	4.82±1.66^a	4.45±2.37^a
SCI-Ex组	8	15.48±2.97	4.47±2.88^a	5.99±5.36^a	9.38±3.25^a,b	10.41±3.08^a,b	10.86±2.62^a,c
F值		0.012	23.036	16.429	41.902	36.036	61.724
P值		0.988	< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	GAD-65	GAD-67
Sham组	4	1.00±0.18	1.00±0.10
SCI-Sed组	4	0.41±0.08^b	0.43±0.07^c
SCI-Ex组	4	0.61±0.09^a,d	0.72±0.06^b,d
F值		22.900	51.693
P值		< 0.001	< 0.001

组别	n	GAD-65	GAD-67
Sham组	4	1.00±0.19	1.00±0.23
SCI-Sed组	4	0.46±0.18^b	0.27±0.18^b
SCI-Ex组	4	0.76±0.14^a,c	0.56±0.15^b,c
F值		30.351	45.162
P值		< 0.001	< 0.001

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Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase (GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury

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