电针风池对急性脑梗死大鼠脑星形胶质细胞和神经元的保护效果

doi:10.3969/j.issn.1006-9771.2021.03.009

Abstract

Abstract: Objective

To investigate the effect of electroacupuncture at Fengchi (GB20) on astrocytes and neurons in rats with acute cerebral infarction.

Methods

A total of 64 male Sprague-Dawley rats were randomly divided into sham group (n = 16), model group (n = 16), non-acupoint group (n = 16) and Fengchi group (n = 16). The latter three groups were modeled with middle cerebral artery occlusion (MCAO), and the latter two groups were treated with electroacupuncture after modeling, at the point near Fengchi and Fengchi, respectively, for seven days. All the rats were assessed with neurological deficit score after treatment, while the cerebral infarction area and water content were measured, the histopathology of hippocampus was observed by HE staining, the expression levels of brain-derived neurotrophic factor (BDNF), glial fibrillary acidic protein (GFAP) and neuron-specific enolase (NSE) in hippocampus were detected with ELISA, the apoptosis of brain neurons were measured with TUNEL, and the expression levels of caspase-3 and Bcl-2 in brain homogenate were detected with Western blotting and reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR).

Results

Compared with the sham group, the neurological deficit score, cerebral infarction area and water content increased significantly (P < 0.05) in the other groups; while the the expression of GFAP and NSE in hippocampus increased (P < 0.05) and the expression of BDNF decreased (P < 0.05); the number of apoptosis in hippocampus increased (P < 0.05), and the expression of caspase-3 increased (P < 0.05) and the expression of Bcl-2 decreased (P < 0.05). Compared with the model group and non-acupoint group, the neurological deficit score, cerebral infarction area and water content decreased in the Fengchi group (P < 0.05); while the the expression of GFAP and NSE in hippocampus decreased (P < 0.05) and the expression of BDNF increased (P < 0.05); the number of apoptosis in hippocampus decreased (P < 0.05), and the expression of caspase-3 decreased (P < 0.05) and the expression of Bcl-2 increased (P < 0.05).

Conclusion

Electroacupuncture at Fengchi can protect the brain from acute cerebral infarction, adjust the expression of GFAP, NSE and BDNF, and reduce the neuronal apoptosis.

Key words: cerebral infarction, electroacupuncture, astrocyte, neurons, rats

CLC Number:

R743.32

Qiong-fen WANG,Feng-bo WANG,Ke WANG,Yong-qiang ZHONG,Jiao-jiao WANG. Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(3): 302-309.

Figures/Tables 8

References 37

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组别	n	评分
假手术组	16	0
模型组	16	2.44±0.51^a
非穴组	16	2.31±0.70^a
风池组	16	1.44±0.63^a,b,c
H值		46.732
P值		< 0.001

组别	n	梗死面积	脑组织含水量
假手术组	4	0	68.68±4.64
模型组	4	47.51±2.20^a	88.28±6.16^a
非穴组	4	46.84±3.10^a	86.73±5.67^a
风池组	4	24.60±2.12^a,b,c	77.30±4.51^a,b,c
统计值		H = 12.918	F = 11.851
P值		0.005	0.001

组别	n	BDNF	GFAP	NSE
假手术组	8	60.59±4.18	293.94±12.28	78.88±8.07
模型组	8	18.25±1.90^a	414.66±18.32^a	154.11±10.69^a
非穴组	8	18.19±2.18^a	413.36±15.73^a	154.43±9.93^a
风池组	8	41.55±2.75^a,b,c	323.48±14.88^a,b,c	97.31±5.74^a,b,c
F值		402.917	128.713	135.543
P值		< 0.001	< 0.001	< 0.001

组别	n	凋亡细胞数	caspase-3 mRNA (2^-ΔΔCt)	Bcl-2 mRNA (2^-ΔΔCt)	caspase-3蛋白 (/GAPDH)	Bcl-2蛋白 (/GAPDH)
假手术组	8	12.00±5.89	0.96±0.20	4.77±0.16	0.11±0.09	0.80±0.11
模型组	8	62.38±12.91^a	7.33±0.98^a	2.46±0.31^a	1.01±0.09^a	0.30±0.10^a
非穴组	8	72.13±10.30^a	7.43±0.74^a	2.91±0.37^a	1.00±0.11^a	0.31±0.10^a
风池组	8	32.63±5.10^a,b,c	4.07±0.67^a,b,c	6.98±0.27^a,b,c	0.37±0.13^a,b,c	2.01±0.10^a,b,c
F值		74.437	57.263	151.894	58.433	194.096
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

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Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction

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