不同线栓法复制局灶性脑缺血模型大鼠恢复期学习记忆能力的差异及其机制

doi:10.3969/j.issn.1006-9771.2022.07.008

Abstract

Abstract:

Objective To explore the difference of learning and memory function between permanent cerebral ischemia and ischemia-reperfusion rat models by filament-occluded method after three weeks of natural recovery, and to observe the changes of brain structure.

Methods A total of 21 SPF male Sprague-Dawley rats were randomly divided into sham group (n = 7), permanent ischemia group (n = 7) and ischemia-reperfusion group (n = 7). The latter two group underwent left middle cerebral artery occlusion, and the ischemia-reperfusion group was reperfused after 90 minutes of ischemia. All the rats were tested with Morris Water Maze 16 to 22 days after modeling, to record escape latency in the navigation experiment, as well as first latency, platform quadrant swimming time ratio and distance ratio, boundary swimming time ratio and distance ratio, average speed, and the swimming path in the space exploration experiment. On the 22nd day after modeling, four rats with similar Longa scores in each group were scanned by magnetic resonance by diffusion tensor imaging, to measure the fractional anisotropy (FA) of cerebral ischemic cortex, striatum, hippocampus and their corresponding contralateral regions, to calculate ratio of FA (rFA); as well as the volume of infarct (VI) and ratio of VI (rVI).

Results For navigation experiment, the escape latency decreased in all the groups with the time (F = 36.202, P < 0.001), but it was not significant for the permanent ischemia group (F = 4.004, P > 0.05). The escape latency was longer in the permanent ischemia group than in the other two groups at each time points (P < 0.05). For space exploration experiment, the boundary time ratio and distance ratio were more in the permanent ischemia group and the ischemia-reperfusion group than in the sham group (P < 0.017), and the first latency was longer in the permanent ischemia group than in the sham group (P < 0.017). The swimming path of ischemia-reperfusion group and sham operation group was better than that of the permanent ischemia group. FA and rFA in left cortex and striatum were less in the permanent ischemia group and the ischemia-reperfusion group than in the sham group (P < 0.05), while FA and rFA of left cortex and FA of left striatum were less in the permanent ischemia group than in the ischemia-reperfusion group (P < 0.05). The VI and rVI were larger in the permanent ischemia group than in the ischemia-reperfusion group (t > 4.185, P < 0.01). FA and rFA of left cortex and striatum correlated with escape latency, boundary time ratio and distance ratio (|r| > 0.498, P < 0.05). The VI and rVI correlated with escape latency and boundary time ratio (|r| > 0.538, P < 0.05).

Conclusion Cognitive impairment is more severe in the rat model of permanent cerebral ischemia, which may relate to the larger damage of structure of nerve fibers in ischemic cortex and striatum, and larger infarct size.

Key words: cerebral ischemia, permanent ischemia, ischemia-reperfusion, learning and memory, rats

CLC Number:

R743.3

MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.

Figures/Tables 8

References 33

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组别	n	第1天	第2天	第3天	第4天	第5天	F值	P值
假手术组	7	48.457±6.43	19.214±12.08^a	12.117±4.00^a	11.099±3.92^a	6.900±1.64^a,b,c,d	44.268	< 0.001
缺血再灌注组	7	62.264±18.56	31.089±24.55^a	26.210±26.22^a	23.166±23.87^a,b	18.526±22.75^a,b	20.376	< 0.001
永久性缺血组	7	81.647±13.73^e,f	72.447±24.47^e,f	64.357±24.90^a,e,f	58.917±26.99^e,f	51.183±37.72^a,e,f	4.004	0.058
F值		10.165	12.172	11.590	9.889	5.696
P值		0.001	< 0.001	0.001	0.001	0.012

组别	n	潜伏期/s	象限时间比/%	象限距离比/%	边界时间比/%	边界距离比/%	速度/m·s^-1
假手术组	7	6.866±3.80	33.921±7.48	28.747±4.69	7.909±3.40	5.211±2.36	0.753±0.16
缺血再灌注组	7	17.101±9.21	30.844±9.76	33.734±9.92	20.759±11.51^a	29.241±30.19^a	0.783±0.13
永久性缺血组	7	55.349±42.68^a	26.346±5.55	27.753±5.33	46.163±35.48^a	42.847±37.79^a	0.810±0.11
χ²值		12.475	4.148	2.167	12.632	11.688	0.925
P值		0.002	0.126	0.338	0.002	0.003	0.630

组别	n	左侧FA	右侧FA	rFA
假手术组	4	0.212±0.01	0.228±0.03	0.937±0.07
缺血再灌注组	4	0.095±0.01^a	0.229±0.02	0.421±0.06^a
永久性缺血组	4	0.069±0.01^a,b	0.216±0.04	0.321±0.04^a,b
F值		251.107	0.237	133.492
P值		< 0.001	0.794	< 0.001

组别	n	左侧FA	右侧FA	rFA
假手术组	4	0.242±0.02	0.262±0.03	0.930±0.07
缺血再灌注组	4	0.121±0.01^a	0.311±0.04	0.396±0.08^a
永久性缺血组	4	0.091±0.01^a,b	0.276±0.03	0.328±0.02^a
F值		96.358	2.235	114.896
P值		< 0.001	0.163	< 0.001

组别	n	左侧FA	右侧FA	rFA
假手术组	4	0.223±0.08	0.225±0.07	1.000±0.08
缺血再灌注组	4	0.235±0.04	0.251±0.03	0.942±0.17
永久性缺血组	4	0.241±0.03	0.236±0.06	1.061±0.25
F值		0.133	0.214	0.445
P值		0.877	0.811	0.654

Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods

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组别	n	梗死体积/mm³	健侧体积/mm³	梗死体积比/%
缺血再灌注组	4	186.823±28.17	946.825±49.67	19.711±2.60
永久性缺血组	4	298.720±32.87^a	953.827±52.13	31.510±5.01^a
t值		5.170	0.194	4.185
P值		0.002	0.852	0.006

DTI参数	逃避潜伏期		边界时间比		边界距离比
DTI参数	r值	P值	r值	P值	r值	P值
皮质左侧FA	-0.791	< 0.001	-0.860	< 0.001	-0.678	0.001
皮质rFA	-0.786	< 0.001	-0.859	< 0.001	-0.707	< 0.001
纹状体左侧FA	-0.665	0.001	-0.731	< 0.001	-0.517	0.020
纹状体rFA	-0.498	0.026	-0.619	0.004	-0.580	0.007
梗死体积	0.538	0.031	0.691	0.003	0.306	0.249
梗死体积比	0.606	0.013	0.682	0.004	0.322	0.223

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