经颅直流电刺激联合任务导向性康复训练对脊髓损伤大鼠前肢运动障碍的效果

doi:10.3969/j.issn.1006-9771.2023.07.006

Abstract

Abstract:

Objective To observe the effect of transcranial direct current stimulation (tDCS) combined with task-oriented rehabilitation training single pellet reaching and grasping (SPG) on the motor function of forelimb in rats with unilateral contusion of C₅ spinal cord.

Methods A total of 60 adult male Sprague-Dawley rats were randomly divided into sham operation group (sham group), spinal cord injury (SCI) group, tDCS group, SPG group, false group and tDCS+SPG group, with ten rats in each group. Only C₅ lamina was removed in the sham group, and the C₅ spinal cord contusion model was established by IH spinal cord impactor in the other five groups. The rats received tDCS in tDCS group, SPG in SPG group, tDCS without current in false group, tDCS combined with SPG in tDCS+SPG group, and no treatment in the SCI and the sham groups. The rats were evaluated with Rearing and Grooming tests, and motor-evoked potential (MEP).

Results Four weeks after operation, compared with SCI group, the scores of Rearing and Grooming increased in tDCS group and tDCS+SPG group (P < 0.05), and they were more in the tDCS+SPG group than in tDCS group and SPG group (P < 0.05); the score of Grooming increased in SPG group (P < 0.05); while the amplitude of MEP increased in tDCS group, SPG group and tDCS+SPG group (P < 0.05), and the latency shortened in tDCS group and tDCS+SPG group (P < 0.05); and the amplitude increased more in tDCS+SPG group than in tDCS group and SPG group (P < 0.01).

Conclusion tDCS could promote the recovery of motor function in rats with SCI, and the combination therapy of tDCS and task-oriented rehabilitation training is more effective.

Key words: spinal cord injury, transcranial direct current stimulation, task-oriented rehabilitation training, motor function, rats

CLC Number:

R651.2

LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.

Figures/Tables 3

References 23

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组别	n	术前	术后4周
假手术组	10	76.80±5.01	76.70±5.12
脊髓损伤组	10	75.70±6.89	30.20±3.22
tDCS组	10	75.90±7.91	41.10±3.32^a
SPG组	10	76.20±7.21	36.20±3.19
假刺激组	10	76.90±5.84	31.20±3.30
tDCS+SPG组	10	74.20±6.99	50.60±2.68^b,c,d
F值		0.214	242.410
P值		0.955	< 0.001

组别	n	术前	术后4周
假手术组	10	5.00±0.00	5.00±0.00
脊髓损伤组	10	5.00±0.00	2.80±0.60
tDCS组	10	5.00±0.00	3.76±0.28^a
SPG组	10	5.00±0.00	3.35±0.37^b
假刺激组	10	5.00±0.00	2.85±0.54
tDCS+SPG组	10	5.00±0.00	4.28±0.37^c,d,e
F值			44.120
P值			< 0.001

组别	n	潜伏期/ms	振幅/μV
假手术组	10	7.18±0.18	49.84±2.19
脊髓损伤组	10	9.20±0.27	6.37±1.32
tDCS组	10	8.09±0.42^a	17.94±1.92^b
SPG组	10	8.90±0.45	9.28±1.23^a
假刺激组	10	9.05±0.36	6.10±0.93
tDCS+SPG组	10	7.77±0.38^b	39.55±3.63^c,d,e
F值		52.440	816.341
P值		< 0.001	< 0.001

Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury

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