线栓法建立卒中后吞咽障碍的大鼠模型

doi:10.3969/j.issn.1006-9771.2023.10.005

Abstract

Abstract:

Objective To explore a feasible animal model of dysphagia after stroke.
Methods Twenty-two clean Sprague-Dawley rats were randomly divided into normal group (n = 11) and model group (n = 11). The model of dysphagia after stroke was established by the thread embolism, and the normal group received no intervention. The latency of the first swallowing attack and the number of swallowing were recorded three and seven days after modeling. The cerebral infarction was detected by TTC staining, and the neuronal apoptosis in ischemic brain was detected by TUNEL fluorescence staining.
Results Compared with the normal group, the swallowing latency prolonged and the number of swallowing reduced three days in the model group, however, there was no significant difference (P > 0.05); seven days after modeling, the swallowing latency prolonged (P < 0.05), and the number of swallowing slightly reduced with little significant difference (P > 0.05). Compared with the normal group, the brain tissue showed obvious infarction area and a large number of apoptotic cells, while the body mass reduced in the model group (P < 0.05).
Conclusion The model rats express some features of dysphagia, which may become a transformation model of dysphagia after stroke.

Key words: stroke, dysphagia, animal model, rats

CLC Number:

R743.3

HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.

Figures/Tables 6

References 35

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组别	n	吞咽潜伏期
正常组	8	5.35(3.73, 5.58)
造模组3 d后	7	17.00(5.00, 30.00)
造模组7 d后	7	32.00(7.00, 34.00)^a

组别	n	吞咽次数
正常组	11	14.00(7.25, 40.00)
造模组3 d后	10	4.50(1.75, 35.75)
造模组7 d后	7	12.00(3.00, 12.00)

组别	n	造模前	造模后第1天	造模后第2天	造模后第3天	造模后第4天	造模后第5天	造模后第6天	造模后第7天
正常组	11	196.15±5.85	204.26±9.93^a	220.53±14.18^a	227.33±13.11^a	226.86±11.50^a	246.90±12.76^a	251.31±15.57^a	264.81±13.26^a
造模组	7	198.83±10.92	181.79±13.48^a,b	172.81±20.99^a,b	169.22±28.39^a,b	168.00±33.96^a,b	176.08±35.77^a,b	179.22±35.87^b	191.09±39.77^b

Establishment of rat model of dysphagia after stroke by thread embolism

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