CJRTP

《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2009, Vol. 15 ›› Issue (11): 1017-1018.

• 基础研究 • Previous Articles     Next Articles

Action Potential of Cardiomyocyte-like Cells Differentiated from Human Marrow Mesenchymal Stem Cells in Vitro

DENG Fang-ge,GUO Lian-feng,MA Ying-zhi,et al   

  1. Key Laboratory for Pathobiology of the Ministry of Education, Jilin University, Changchun 130021, Jilin, China；State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Diseases, the First Affiliated Hospital of Guangzhou Medical Co
  • Received:2009-08-25 Revised:2009-09-27 Published:2009-11-01 Online:2009-11-01

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Abstract: Objective To detect whether the cardiomyocyte-like cells differentiated from human marrow mesenchymal stem cells (hMSCs) could produce action potential (AP). Methods Isolated and cultured hMSCs were induced into cardiomyocyte-like cells with 5-Azacytine in vitro. They were measured for their AP by patch clamp technique, and compared with those of hMSCs of the same generation and beating cardiomyocytes (CMs) derived from 2 day-old SD rats. Results 6/30 cardiomyocyte-like cells produced AP. The CMs produced significant AP, hMSCs appeared no AP, and cardiomyocyte-like cells appeared weak AP. Conclusion The hMSCs manifested the potential to differentiate into CMs in the electrophysiology characteristics following 5-Azacytine induction.

Key words: human mesenchymal stem cells (hMSCs), cardiomyocyte-like cells, patch clamp, action potential (AP), excitability