壳聚糖-碱性成纤维细胞生长因子载体诱导骨髓间充质干细胞向神经细胞分化

Abstract

Abstract: ObjectiveTo explore the effect of bFGF-chitosan carriers on inducing bone marrow-derived mesenchymal stem cells (MSCs) to differentiate into nerve cells.MethodsMSCs were detected by immunohistochemistry and Western blot after they were induced by bFGF-chitosan carriers to differentiate into neurons. The MTT chromometry assay was carried out to determine cell viability.ResultsThe proportion of express neural stem cells marker Nestin, and neuronal markers class Ⅲ β-tubulin and MAP-2 was 83.54% after MSCs induced by bFGF-chitosan carriers.ConclusionbFGF-chitosan carriers can induce MSCs to differentiate into nerve cells with a high percentage.

Key words: bone marrow-derived mesenchymal stem cells, basic Fibroblast Growth Factor, chitosan, induction, neural differentiation, cytokine, nerve cells

DUAN Hong-mei;YANG Zhao-yang;LI Xiao-guang.. Basic Fibroblast Growth Factor-chitosan Carriers Induce Bone Marrow-Derived Mesenchymal Stem Cells to Differentiate into Nerve Cells[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2011, 17(4): 329-333.

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Basic Fibroblast Growth Factor-chitosan Carriers Induce Bone Marrow-Derived Mesenchymal Stem Cells to Differentiate into Nerve Cells

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