《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2009, Vol. 15 ›› Issue (04): 327-329.
• 基础研究 • Previous Articles Next Articles
WANG Jin-guang, JING Xin, ZHANG Jian-min, et al
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Abstract: Objective To investigate the effects of RNA interference (RNAi) on Caspase-3 expression.Methods According to the gene sequence and the secondary structure characteristics of Caspase-3, three siRNA (Si-Caspase-3-1, Si-Caspase-3-2 and Si-Caspase-3-3) were designed with 21 basic group, and siRNAs against Caspase-3 gene were synthesized. With the liposome conduct, the synthesized siRNAs were transferred into neuroglia cells. After the cells crept the slices, cells were dyed with fluor Hoechst33258; the apoptotic cells were stained with TUNEL, the normal neuroglia cells were as control, the Caspase-3 protease activity were detected with hemi-quantitative RT-PCR and Western blot.Results The synthesized siRNAs could inhibit the expression of Caspase-3 gene. For Si-Caspase-3-3, the expression inhibition rate of Caspase-3 mRNA was 86.32% in neuroglia cell.Conclusion The synthesized siRNAs can inhibit the expression of Caspase-3 gene at cell level.
Key words: RNA inference, inhibitive effect, Caspase-3
WANG Jin-guang;JING Xin;ZHANG Jian-min;et al. Inhibitive Effects of RNA Interference on Caspase-3 Gene Expression[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2009, 15(04): 327-329.
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