Abstract: Objective To investigate the effects of morroniside on hydrogen peroxide (H₂O₂)-induced apoptosis in SH-SY5Y neuroblastoma cells. Methods SH-SY5Y cells were pre-incubated with morroniside (1, 10, and 100 μmol/L) for 24 h prior to exposure to H₂O₂ (300～500 μmol/L) for 18 h. The content of malondialdehyde (MDA)，mitochondrial membrane potentials (MMP) and apoptosis ratio were determined. Results Pretreatment the cells with morroniside (1, 10 and 100 μmol/L) lowered the H₂O₂-induced MDA concentration and MMP, and inhibited the apoptosis. Conclusion Morroniside may protect the neurocyte from H₂O₂-induced apoptosis by lowered MDA and inhibits MMP.

Key words: morroniside, SH-SY5Y neuroblastoma cell, lipid peroxidation, mitochondrial membrane potentials, apoptosis