《Chinese Journal of Rehabilitation Theory and Practice》 ›› 2014, Vol. 20 ›› Issue (6): 527-532.
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MINHong-wei, LIUKe-min,WANGAn-qing,etal.
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Abstract: ObjectiveToexplorethemethodtoestablishanimalmodelofearlyosteonecrosisofthefemoralhead(ONFH)inducedby steroid.Methods20healthymaleBeagleswererandomlydividedintocontrolgroupandexperimentalgroupwith10dogsineachgroup. Theexperimentalgroupwasinjectedlipopolysaccharide10μg/kgandmethylprednisolone20mg/kgfor3daysconsecutively.Thecontrol groupwasinjectednormalsaline.2monthsand4monthsafteradministration,bothgroupswereperformedmagneticresonanceimaging (MRI).5animalsweresacrificedrespectivelyat2monthsand4monthsafteradministrationineachgroup,andbilateralfemoralheadspeci-menswereobtainedtoperformhistologicalexamination.Plasmaprothrombintime(PT),activatedpartialthromboplastintime(APTT),anti-thrombinIII(AT-III)weretestedbeforeand24hafteradministration.ResultsIntheexperimentalgroup,thepathologicalresultsshowed thattherewere4ONFH2monthsand6ONFH4monthsafteradministrationandMRIdidnotshowanyabnormality.Comparedwiththe controlgroup,thePT,APTT,AT-IIIintheexperimentgroupshortenedsignificantlyafteradministration(P<0.001).ConclusionModified steroid-inducedmethodcanestablishtheanimalmodelofearlyONFH.Hypercoagulationandlowfibrinolysismaybethereasonofste-roid-inducedosteonecrosis.
Key words: osteonecrosisofthefemoralhead, earlystage, modifiedsteroid-inducedmethod, animalmodel, Beagles
MINHong-wei;LIUKe-min;WANGAn-qing;etal.. EstablishmentofAnimalModelofEarlyOsteonecrosisoftheFemoralHeadwithModified-inducedSteroidMethod[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2014, 20(6): 527-532.
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https://www.cjrtponline.com/EN/Y2014/V20/I6/527