次声对大鼠海马区钙调依赖性蛋白激酶Ⅱ及Tau蛋白表达的影响

doi:10.3969/j.issn.1006-9771.2017.03.010

Abstract

Abstract: ObjectiveTo study the effect of infrasound on expression of calmodulin-dependent protein kinase II (CaMKII) and tau protein in hippocampus of rats. MethodsFifty-six male Sprague-Dawley rats were randomized into control group (n=8), 1-day group (n=8), 7-day group (n=8) and 14-day group (n=32), and the 14-day group was subgrouped as 1-hour, 6-hour, 24-hour, 48-hour subgroups, naming after the time after infrasound exposure, 8 in each subgroup. All the test groups were put in an infrasound field with 8 Hz, 130 dB for 2 hours daily, while the control group was put in the infrasound instrument without infrasound exposure for 2 hours daily. The expression of pT²⁸⁶-CaMKII and tau protein in hippocampus was detected with immunohistochemisty, Western blotting and enzyme-linked immunoabsorbent assay. ResultsThe expression of pT²⁸⁶-CaMKII was the most in 14-day group (F>14.912, P<0.001), as well as the expression of tau protein (F>36.229, P<0.001), and secondary in 7-day group (P<0.05). For 14-day group, the expression of tau protein was the most in 1-hour and 6-hour subgroups, and dropped down in 24-hour subgroup, although more than that in the control group (P<0.05). ConclusionExposure of 8 Hz, 130 dB infrasound may induce phosphorylation of CaMKII and tau protein, and the expression of tau protein in hippocampal cells in rat, which may disturb their learning and memory function.

Key words: infrasound, calmodulin-dependent protein kinase II, tau protein, learning and memory, hippocampus, rats

CLC Number:

R594.9

CAI Li, WANG Jin, CUI Yao, ZHANG Ge, WU Song-yan, ZHANG Wen-dong, LIU Zhao-hui. Effects of Infrasound on Expression of Calmodulin-dependent Protein Kinase II and Tau Protein in Hippocampus of Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2017, 23(3): 298-303.

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Effects of Infrasound on Expression of Calmodulin-dependent Protein Kinase II and Tau Protein in Hippocampus of Rats

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