雷公藤内酯醇对阿尔茨海默病大鼠海马drebrin和cofilin表达的影响

doi:10.3969/j.issn.1006-9771.2018.01.005

Abstract

Abstract: Objective To observe the effects of triptolide on drebrin and cofilin expression in the hippocampus of rats with Alzheimer's disease (AD).Methods Sixty male Sprague-Dawley rats were equally divided into control group, model group and triptolide-treated group with 20 cases in each group. The AD model was established with unilateral injection of beta amyloid 1-40 (Aβ_1-40) into hippocampus in rats. The control group was established with unilateral injection of normal saline with the same volume into hippocampus in rats. The triptolide-treated group was administered triptolide intraperitoneally, 0.4 mg/kg, once a day, for 15 days after modeling. Spine density of hippocampal neurons was assayed by Golgi staining. Drebrin and cofilin expression of hippocampal neurons was assayed by immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR).Results The spine density of hippocampal neurons was higher in the triptolide-treated group than in the model group (P<0.05). The average optical density of drebrin was higher in the triptolide-treated group than in the the model group (P<0.01), while the cell number and average optical density of cofilin were lower (P<0.05). The drebrin mRNA expression was higher in the triptolide-treated group than in the model group (P<0.05), and the cofilin mRNA expression was lower (P<0.01).Conclusion Triptolide may delay the degeneration of dendritic spines in hippocampal neurons of AD rats by regulating the expression of drebrin and cofilin.

Key words: Alzheimer's disease, triptolide, drebrin, cofilin, rats

CLC Number:

R749.1

ZHANG Sai-sheng, YANG Bao-lin, CHENG Li-xia, WAN Bin, NIE Jing, HU Xiao-ling, LÜ Cheng. Effects of Triptolide on Expression of Drebrin and Cofilin in Hippocampus of Rats with Alzheimer's Disease[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(1): 23-28.

References

[1] Kepp KP. Ten challenges of the amyloid hypothesis of Alzheimer's disease [J]. J Alzheimers Dis, 2017, 55(2): 447-457.
[2] McGeer PL, McGeer EG. Targeting microglia for the treatment of Alzheimer's disease [J]. Expert Opin Ther Targets, 2015, 19(4): 497-506.
[3] Minter MR, Taylor JM, Crack PJ. The contribution of neuroinflammation to amyloid toxicity in Alzheimer's disease [J]. J Neurochem, 2016, 136(3): 457-474.
[4] Lynch MA. Neuroinflammatory changes negatively impact on LTP: A focus on IL-1β [J]. Brain Res, 2015, 1621: 197-204.
[5] Jebelli J, Hooper C, Pocock JM. Microglial p53 activation is detrimental to neuronal synapses during activation-induced inflammation: Implications for neurodegeneration [J]. Neurosci Lett, 2014, 583: 92-97.
[6] Cui YQ, Wang Q, Zhang DM, et al. Triptolide rescues spatial memory deficits and amyloid-β aggregation accompanied by inhibition of inflammatory responses and MAPKs activity in APP/PS1 transgenic mice [J]. Curr Alzheimer Res, 2016, 13(3): 288-296.
[7] Wang Q, Xiao B, Cui S, et al. Triptolide treatment reduces Alzheimer's disease (AD)-like pathology through inhibition of BACE1 in a transgenic mouse model of AD [J]. Dis Model Mech, 2014, 7(12): 1385-1395.
[8] Wan B, Hu X, Nie J, et al. Effects of triptolide on degeneration of dendritic spines induced by Aβ 1-40 injection in rat hippocampus [J]. Neurol Sci, 2014, 35(1): 35-40.
[9] Rangamani P, Levy MG, Khan S, et al. Paradoxical signaling regulates structural plasticity in dendritic spines [J]. Proc Natl Acad Sci U S A, 2016, 113(36): E5298-E5307.
[10] 马丽娜,王蓉. Drebrin与认知功能障碍[J]. 中国比较医学杂志, 2013, 23(6): 67-70.
[11] Noguchi J, Hayama T, Watanabe S, et al. State-dependent diffusion of actin-depolymerizing factor/cofilin underlies the enlargement and shrinkage of dendritic spines [J]. Sci Rep, 2016, 6: 32897.
[12] Bie B, Wu J, Yang H, et al. Epigenetic suppression of neuroligin 1 underlies amyloid-induced memory deficiency [J]. Nat Neurosci, 2014, 17(2): 223-231.
[13] 刘若兰,沈沉,沈晓燕,等.电针对阿尔茨海默病大鼠海马谷氨酸受体相互作用蛋白1、2表达的影响[J]. 中国康复理论与实践, 2016, 22(4): 413-416.
[14] Chang PK, Boridy S, McKinney RA, et al. Letrozole potentiates mitochondrial and dendritic spine impairments induced by β amyloid [J]. J Aging Res, 2013, 2013: 538979.
[15] Perez-Cruz C, Nolte MW, van Gaalen MM, et al. Reduced spine density in specific regions of CA1 pyramidal neurons in two transgenic mouse models of Alzheimer's disease [J]. J Neurosci, 2011, 31(10): 3926-3934.
[16] Grutzendler J, Helmin K, Tsai J, et al. Various dendritic abnormalities are associated with fibrillar amyloid deposits in Alzheimer's disease [J]. Ann N Y Acad Sci, 2007, 1097: 30-39.
[17] Li Y, Liu L, Barger SW, et al. Interleukin-1 mediates pathological effects of microglia on tau phosphorylation and on synaptophysin sythesis in cortical neurons through a p38-MAPK pathway [J]. J Neurosci, 2003, 23(5): 1605-1611.
[18] Wang QW, Rowan MJ, Anwyl R. Inhibition of LTP by beta-amyloid is prevented by activation of beta2 adrenoceptors and stimulation of the cAMP/PKA signalling pathway [J]. Neurobiol Aging, 2009, 30(10): 1608-1613.
[19] Tong L, Prieto GA, Kramár EA, et al. Brain-derived neurotrophic factor-dependent synaptic plasticity is suppressed by interleukin-1β via p38 mitogen-activated protein kinase [J]. J Neurosci, 2012,32(49): 17714-17724.
[20] 柴继侠,于剑锋,陈明军,等. β-淀粉样蛋白和drebrin在培养的APP/PS1转基因小鼠神经元中的表达[J]. 解剖学杂志, 2010, 33(4): 502-505.
[21] Julien C, Tremblay C, Bendjelloul F, et al. Decreased drebrin mRNA expression in Alzheimer disease: correlation with tau pathology [J]. J Neurosci Res, 2008, 86(10): 2292-2302.
[22] Yao J, Hennessey T, Flynt A, et al. MicroRNA-related cofilin abnormality in Alzheimer's disease [J]. PLoS One, 2010, 5(12): e15546.
[23] Kojima N, Shirao T. Synaptic dysfunction and disruption of postsynaptic drebrin-actin complex: A study of neurological disorders accompanied by cognitive deficits [J]. Neurosci Res, 2007, 58: 1-5.
[24] 吴娜,刘星雨,王笃军,等. 雷公藤内酯醇对C57BL/6小鼠亚慢性毒性作用的研究[J]. 中成药, 2014, 36(5): 904-908.
[25] Zeng Y, Zhang J, Zhu Y, et al. Tripchlorolide improves cognitive deficits by reducing amyloid β and upregulating synapse-related proteins in a transgenic model of Alzheimer's disease [J]. J Neurochem, 2015, 133(1): 38-52.
[26] Lin N, Chen LM, Pan XD, et al. Tripchlorolide attenuates β-amyloid generation via suppressing PPARγ-regulated BACE1 activity in N2a/APP695 Cells [J]. Mol Neurobiol, 2016, 53(9): 6397-6406.

Effects of Triptolide on Expression of Drebrin and Cofilin in Hippocampus of Rats with Alzheimer's Disease

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	LI Fang, HUO Su, DU Jubao, LIU Xiuzhen, LI Xiaoshuang, SONG Weiqun. Effect of transcranial direct current stimulation combined with task-oriented rehabilitation training on forelimb motor dysfunction in rats with spinal cord injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(7): 777-781.
[2]	HUANG Zhilin, XU Fashao, SHI Jing, HUANG Gan, LIU Meifang, ZHANG Xiahui. Establishment of rat model of dysphagia after stroke by thread embolism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(10): 1147-1153.
[3]	QIN Yanqiang, DONG Hao, SUN Yingchun, CHENG Xiankuan, YAO Haijiang. Effects of different acupuncture schemes on neurotransmitters and related inflammatory factors in rats with post-stroke depression [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2023, 29(1): 30-37.
[4]	MIAO Pei,ZHANG Tong,MI Haixia,ZHANG Weidong. Learning and memory ability and its mechanism in rats with focal cerebral ischemia induced by two filament-occluded methods [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(7): 789-796.
[5]	ZHOU Xiaojue,FENG Jing,PANG Rizhao,LIU Jie,ZHANG Anren. Every-other-day fasting attenuated inflammation in rats after spinal cord injury via the aryl hydrocarbon receptor/suppressor of cytokine signaling 2/nuclear transcription factor-κB signaling pathway [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 544-551.
[6]	SONG Shaofei,HOU Yuanyuan,WANG Yunlei,ZHANG Tong. Effects of circadian misalignment induced by abnormal photoperiod on expression rhythm of clock genes and glucose uptake related genes in gastrocnemius in rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(5): 552-558.
[7]	LI Tong,FANG Zhipeng,SHAO Yuping,WANG Ping. Effect of aerobic exercise on learning and memory, and synaptic plasticity of hippocampal neurons for sleep-deprived rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2022, 28(11): 1270-1277.
[8]	Jing-yi WANG,Jie YIN,Jian-cheng LIU,Ri-zhao PANG,Wen-chun WANG. Effect of Iridoid-rich Fraction from Valeriana Jatamansi Jones on Neuron Pyroptosis in Rats with Acute Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(6): 653-660.
[9]	Wen-mei ZHOU,Tao TAO,Shuang WU,Ting-long WANG,Zheng-yi YANG,Ying ZHANG. Effects of Enriched Environment on Neurological Function and Glucose Metabolism in Ischemic Penumbra in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(5): 522-529.
[10]	Qiong-fen WANG,Feng-bo WANG,Ke WANG,Yong-qiang ZHONG,Jiao-jiao WANG. Effect of Electroacupuncture at Fengchi on Astrocytes and Neurons in Rats with Acute Cerebral Infarction [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(3): 302-309.
[11]	Xiang-zhe LI,Jie DING,Qing-hua WANG,Chuan-ming DONG,Tong WANG,Qin-feng WU. Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase (GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 131-136.
[12]	Kun AI,Ming XU,Qiong LIU,Shi-feng DENG,Ji-sheng LIU,Fang QI,Xi-qin YI,Qi-rui QU,Hong ZHANG. Effect of Electroacupuncture on Content of Cyclic Adenosine Monophosphate and Protein Kinase A, and Phosphorylation of Myosin Light Chain Kinase in Detrusor of Rats with Detrusor Hyperreflex after Suprasacral Spinal Cord Injury [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(2): 137-144.
[13]	LING Meng-yu,YANG Yi-zhuo,LIU Shuai,YE Chao-qun. Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(11): 1272-1281.
[14]	PENG Zhi-feng, ZHANG Yi-ping, ZHANG Ji-hong. Effect of Early Exercise Combined with Pentylenetetrazol on Neurologic Function after Cerebral Ischemia-reperfusion in Rats and Its Mechanism [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2021, 27(1): 27-30.
[15]	CHEN Xiao-ping,WANG Ming-yang,MA Deng-lei,GONG Shi-li,ZHANG Li,LI Ya-li,LI Lin,HU Chao-ying,ZHANG Lan. Effects of Analgecine on Apoptosis and Neuroinflammation in Cerebral Ischemia-reperfusion Injury Rats [J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(9): 1038-1044.