Ski对脂多糖诱导大鼠星形胶质细胞炎症因子释放的影响

doi:10.3969/j.issn.1006-9771.2018.07.008

Abstract

Abstract: Objective To investigate the effect of Ski on the secretion of inflammatory cytokines from activated astrocytes. Methods Astrocytes were obtained from cerebral cortex of a three-day old Sprague-Dawley rat and cultured in vitro. They were divided into blank group, control group and siRNA group. The Ski gene was silenced in siRNA group. The expression of Ski was tested with Western blotting and immunofluorescence 48 hours later. Then the astrocytes were stimulated with lipopolysaccharide for 24 hours. The secretion of tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in activated astrocytes was detected with ELISA. Results The expression of Ski protein reduced in the siRNA group (P<0.001), as well as the secretion of TNF-α and IL-1β (P<0.001). Conclusion Ski may play a role in inflammatory response of astrocyte.

Key words: Ski, RNA interference, lipopolysaccharide, inflammation, astrocytes, rats

CLC Number:

R741

WANG Ming, YANG Xin-le, CHEN Tie-ge, ZHANG Dong-liang, GONG Zhao-yang, XIANG Gao, LIU Kai-xin, ZHANG Hai-hong. Role of Ski in Inflammatory Factor Release in Activated Astrocytes[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(7): 802-806.

References

[1] Mietto BS, Mostacada K, Martinez AM.Neurotrauma and inflammation: CNS and PNS responses[J]. Mediators Inflamm, 2015, 2015: 251204.
[2] Lukovic D, Stojkovic M, Moreno-Manzano V, et al.Concise review: reactive astrocytes and stem cells in spinal cord injury: good guys or bad guys?[J]. Stem Cells, 2015, 33(4): 1036-1041.
[3] Messmer K, Reynolds GP.An in vitro model of inflammatory neurodegeneration and its neuroprotection[J]. Neurosci Lett, 2005, 388(1): 39-44.
[4] Pekny M, Wilhelmsson U, Pekna M.The dual role of astrocyte activation and reactive gliosis[J]. Neurosci Lett, 2014, 565: 30-38.
[5] Sofroniew MV.Astrogliosis[J]. Cold Spring Harb Perspect Biol, 2014, 7(2): a020420.
[6] Cerbai F, Lana D, Nosi D, et al.The neuron-astrocyte-microglia triad in normal brain ageing and in a model of neuroinflammation in the rat hippocampus[J]. PLoS One, 2012, 7(9): e45250.
[7] Li Y, Turck CM, Teumer JK, et al.Unique sequence, Ski, in Sloan-Kettering avian retroviruses with properties of a new cell-derived oncogene[J]. J Virol, 1986, 57(3): 1065-1072.
[8] Stavnezer E, Barkas AE, Brennan LA, et al.Transforming Sloan-Kettering viruses generated from the cloned v-Ski oncogene by in vitro and in vivo recombinations[J]. J Virol, 1986, 57(3): 1073-1083.
[9] Bonnon C, Atanasoski S. c-Ski in health and disease[J]. Cell Tissue Res, 2012, 347(1): 51-64.
[10] 周开升,朱彦东,张海鸿,等. Ski在神经系统中的作用及机制的研究进展[J]. 中国康复理论与实践, 2016, 22(7): 797-800.
[11] 赵鑫,郭永强,王明,等. 脂多糖对大鼠星形胶质细胞Ski蛋白表达的影响[J]. 中国康复理论与实践, 2017, 23(5): 514-519.
[12] Gong P, Xu X, Shi J, et al.Phosphorylation of mitogen- and stress-activated protein kinase-1 in astrocytic inflammation: a possible role in inhibiting production of inflammatory cytokines[J]. PLoS One, 2013, 8(12): e81747.
[13] Cheng KY, Liu Y, Han YG, et al.Follistatin-like protein 1 suppressed pro-inflammatory cytokines expression during neuroinflammation induced by lipopolysaccharide[J]. J Mol Histol, 2017, 48(2): 63-72.
[14] Cerciat M, Unkila M, Garcia-Segura LM, et al.Selective estrogen receptor modulators decrease the production of interleukin-6 and interferon-gamma-inducible protein-10 by astrocytes exposed to inflammatory challenge in vitro[J]. Glia, 2010, 58(1): 93-102.
[15] Salminen A, Ojala J, Kaarniranta K, et al.Astrocytes in the aging brain express characteristics of senescence-associated secretory phenotype[J]. Eur J Neurosci, 2011, 34(1): 3-11.
[16] Sofroniew MV.Multiple roles for astrocytes as effectors of cytokines and inflammatory mediators[J]. Neuroscientist, 2014, 20(2): 160-172.
[17] Barnett SC, Linington C.Myelination: do astrocytes play a role?[J]. Neuroscientist, 2013, 19(5): 442-450.
[18] Cheeran MC, Hu S, Sheng WS, et al.Differential responses of human brain cells to West Nile virus infection[J]. J Neurovirol, 2005, 11(6): 512-524.
[19] Zhang X, Shi LL, Gao X, et al.Lentivirus-mediated inhibition of tumour necrosis factor-α improves motor function associated with PRDX6 in spinal cord contusion rats[J]. Sci Rep, 2015, 5: 8486.
[20] Zhang X, Wang J, Qian W, et al.Dexmedetomidine inhibits tumor necrosis factor-alpha and interleukin 6 in lipopolysaccharide-stimulated astrocytes by suppression of c-Jun N-terminal kinases[J]. Inflammation, 2014, 37(3): 942-949.
[21] Heiman A, Pallottie A, Heary RF, et al.Toll-like receptors in central nervous system injury and disease: a focus on the spinal cord[J]. Brain Behav Immun, 2014, 42: 232-245.
[22] Li P, Liu P, Xiong RP, et al.Ski, a modulator of wound healing and scar formation in the rat Skin and rabbit ear[J]. J Pathol, 2011, 223(5): 659-671.
[23] Zhao X, Wang XW, Zhou KS, et al.Expression of Ski and its role in astrocyte proliferation and migration[J]. Neuroscience, 2017, 362: 1-12.
[24] Prunier C, Pessah M, Ferrand N, et al.The oncoprotein Ski acts as an antagonist of transforming growth factor-beta signaling by suppressing Smad2 phosphorylation[J]. J Biol Chem, 2003, 278(28): 26249-26257.
[25] Makwana M, Jones LL, Cuthill D, et al.Endogenous transforming growth factor beta 1 suppresses inflammation and promotes survival in adult CNS[J]. J Neurosci, 2007, 27(42): 11201-11213.
[26] Wake H, Moorhouse AJ, Nabekura J.Functions of microglia in the central nervous system–beyond the immune response[J]. Neuron Glia Biol, 2011, 7(1): 47-53.
[27] Street ME, Miraki-Moud F, Sanderson IR, et al.Interleukin-1beta (IL-1beta) and IL-6 modulate insulin-like growth factor-binding protein (IGFBP) secretion in colon cancer epithelial (Caco-2) cells[J]. J Endocrinol, 2003, 179(3): 405-415.

Role of Ski in Inflammatory Factor Release in Activated Astrocytes

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