嗅黏膜神经干细胞对大鼠神经元凋亡的影响

doi:10.3969/j.issn.1006-9771.2018.09.007

Abstract

Abstract: Objective To investigate the effect of olfactory mucosal neural stem cells (OM-NSCs) on neuronal apoptosis in rats. Methods OM-NSCs were isolated from adult male Sprague-Dawley rats, and neurons were isolated from fetal Sprague-Dawley rats. The neurons were divided into blank group, control group and experiment group after culture in vitro, which were further cultured in nomal, cultured in nomal 24 hours after stimulation of interleukin-1β (IL-1β), and cultured with OM-NSCs 24 hours after stimulation of IL-1β, respectively, for 24 hours. The apoptosis of neurons was observed with double staining of TUNEL and microtubule-associated protein 2 antibodies. Results There were few apoptotic cells in the blank group, and very many in the control group; the apoptotic cells were fewer in the experiment group than in the control group (F=39.764, P<0.01).Conclusion OM-NSCs can significantly inhibit neuronal apoptosis, which may play a role in neuroprotection.

Key words: olfactory mucous neural stem cells, neurons, apoptosis, rats

CLC Number:

R651.2

YANG Xin-le, WANG Ming, YANG Cheng-wei, ZHANG Ya-qiang, XU Ya-jie, GONG Dong, ZHEN Ping. Effect of Olfactory Mucosal Neural Stem Cells on Neuronal Apoptosis in Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2018, 24(9): 1027-1033.

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Effect of Olfactory Mucosal Neural Stem Cells on Neuronal Apoptosis in Rats

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