痘苗病毒致炎兔皮提取物对脑缺血再灌注脑损伤大鼠凋亡和神经炎症的影响

doi:10.3969/j.issn.1006-9771.2020.09.008

Abstract

Abstract:

Objective To observe the effects of Analgecine (AGC) on middle cerebral artery ischemia-reperfusion injury in rats and its mechanism.
Methods A total of 61 Sprague-Dawley rats were divided into sham group (n = 11), sham-AGC group (n = 11), model group (n = 20) and model-AGC group (n = 19). The model group and the model-AGC group were occluded the middle cerebral arteries for 1.5 hours and reperfused (2 rats in each group unsuccessful). The sham-AGC group and the model-AGC group were injected AGC 20 U/kg through tail-vein, while the sham group and the model group were injected saline of same volume. Four rats in each group were tested heat shock proteins 70 (HSP70), Bcl-2 and Bax in brain with Western blotting 48 hours after injection. The other rats were assessed with Prehensile Traction Test seven days after injection, and then, four of each group were detected ionized calcium binding adapter molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) expression with immunohistochemistry.
Results The prehensile time increased in the model-AGC group compared with that of the model group (P < 0.01), with the increase of HSP70 and Bcl-2 ( P < 0.01) and decrease of Iba1 and GFAP expression ( P < 0.05).
Conclusion AGC may promote the recovery of motor function in rats with cerebral ischemia-reperfusion injury, which may associate with inhibiting cell apoptosis and neruoinflammatory response.

Key words: ischemic stroke, cerebral ischemia-reperfusion, Analgecine, motor, apoptosis, inflammation, rats

CHEN Xiao-ping,WANG Ming-yang,MA Deng-lei,GONG Shi-li,ZHANG Li,LI Ya-li,LI Lin,HU Chao-ying,ZHANG Lan. Effects of Analgecine on Apoptosis and Neuroinflammation in Cerebral Ischemia-reperfusion Injury Rats[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(9): 1038-1044.

Figures/Tables 6

References 34

[1]	Corbyn Z. Statistics: a growing global burden[J]. Nature, 2014, 510(7506):S2-S3. doi: 10.1038/510S2a
[2]	Ribeiro P W, Cola P C, Gatto A R, et al. Relationship between dysphagia, National Institutes of Health Stroke Scale score, and predictors of pneumonia after ischemic stroke[J]. J Stroke Cerebrovasc Dis, 2015, 24(9):2088-2094. doi: 10.1016/j.jstrokecerebrovasdis.2015.05.009
[3]	Qu Y, Zhang H L, Zhang X P, et al. Arachidonic acid attenuates brain damage in a rat model of ischemia/reperfusion by inhibiting inflammatory response and oxidative stress[J]. Hum Exp Toxicol, 2018, 37(2):135-141. doi: 10.1177/0960327117692134 pmid: 29233001
[4]	Wen L, Liu L, Tong L, et al. NDRG4 prevents cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis[J]. Genes Dis, 2019, 6(4):448-454.
[5]	Stegner D, Klaus V, Nieswandt B. Platelets as modulators of cerebral ischemia/reperfusion injury[J]. Front Immunol, 2019, 10:2505. doi: 10.3389/fimmu.2019.02505 pmid: 31736950
[6]	Granger D N, Kvietys P R. Reperfusion injury and reactive oxygen species: the evolution of a concept[J]. Redox Biol, 2015, 6:524-551. doi: S2213-2317(15)00110-X pmid: 26484802
[7]	Akbari-Kordkheyli V, Abbaszadeh-Goudarzi K, Nejati-Laskokalayeh M, et al. The protective effects of silymarin on ischemia-reperfusion injuries: a mechanistic review[J]. Iran J Basic Med Sci, 2019, 22(9):968-976. doi: 10.22038/ijbms.2019.34284.8147 pmid: 31807239
[8]	杨晓梅, 何荣国. 牛痘疫苗致炎兔皮提取物治疗老年带状疱疹后遗神经痛及对血清IL-6的影响[J]. 南方医科大学学报, 2007(12):1941-1943.
	Yang X M, He R G. Effect of extracts from rabbit skin inflamed by vaccinia virus in the management of postherpetic neuralgia and on serum interleukin-6 level in aged patients[J]. J South Med Univ, 2007(12):1941-1943.
[9]	Zhu M, Zhou F, Li L, et al. Success with neurotropin in treating pediatric lower extremity pain induced by spinal cord injury after epidural anesthesia[J]. J Pain Res, 2017, 10:1391-1394. doi: 10.2147/JPR
[10]	Dong J, Tu H P, Ding W Y, et al. Analgecine, the extracts of vaccinia inoculated rabbit skin, effectively alleviates the chronic low back pain with little side effect: a randomized multi-center double-blind placebo-controlled phase 3 clinical trial[J]. Contemp Clin Trials Commun, 2016, 2:16-24. doi: 10.1016/j.conctc.2015.11.002
[11]	Danno D, Kawabata K, Tachibana H. Three cases of nummular headche effectively treated with Neurotropin^®[J]. Intern Med, 2013, 52(4):493-495. doi: 10.2169/internalmedicine.52.9161
[12]	陈明桧, 胡朝英, 李林. 牛痘疫苗接种家兔炎症皮肤提取物的临床应用进展[J]. 中国康复理论与实践, 2019, 25(2):192-195.
	Chen M G, Hu C Y, Li L. Advance in clinical application of neurotropin (review)[J]. Chin J Rehabil Theory Pract, 2019, 25(2):192-195.
[13]	Taneda K, Tominaga M, Tengara S, et al. Neurotropin inhibits both capsaicin-induced substance P release and nerve growth factor-induced neurite outgrowth in cultured rat dorsal root ganglion neurons[J]. Clin Exp Dermatol, 2010, 35(1):73-77. doi: 10.1111/j.1365-2230.2009.03636.x pmid: 19874332
[14]	Liu M Y, Huang X E. Effects of analgecine on oxaliplatin-induced neurotoxicity in patients with gastrointestinal cancer[J]. Asian Pac J Cancer Prev, 2015, 16(10):4465-4468. doi: 10.7314/APJCP.2015.16.10.4465
[15]	Thal S C, Mebmer K, Schmid-Elsaesser R, et al. Neurological impairment in rats after subarachnoid hemorrhage: a comparison of functional tests[J]. J Neurol Sci, 2008, 268(1-2):150-159. doi: 10.1016/j.jns.2007.12.002
[16]	Enzmann G, Kargaran S, Engelhardt B. Ischemia-reperfusion injury in stroke: impact of the brain barriers and brain immune privilege on neutrophil function[J]. Ther Adv Neurol Disord, 2018, 11:1756286418794184.
[17]	Hao M Q, Xie L J, Leng W, et al. Trim47 is a critical regulator of cerebral ischemia-reperfusion injury through regulating apoptosis and inflammation[J]. Biochem Biophys Res Commun, 2019, 515(4):651-657. doi: 10.1016/j.bbrc.2019.05.065
[18]	李春阳. 山茱萸环烯醚萜苷防治缺血性脑血管病的药理机制研究[D]. 北京:首都医科大学, 2006.
	Li C Y. Effects and their mechanisms of cornel iridoid glycoside on experimental ischemic cerebrovascular disease[D]. Beijing: Capital Medical University, 2006.
[19]	Song H, Zhang X, Chen R, et al. Cortical neuron-derived exosomal microRNA-181c-3p inhibits neuroinflammation by downregulating CXCL1 in astrocytes of a rat model with ischemic brain injury[J]. Neuroimmunomodulation, 2019, 26(5):217-233. doi: 10.1159/000502694
[20]	Perez-Alvarez M J, Villa Gonzalez M, Benito-Cuesta I, et al. Role of mTORC1 controlling proteostasis after brain ischemia[J]. Front Neurosci, 2018, 12:60. doi: 10.3389/fnins.2018.00060
[21]	Ashabi G, Khalaj L, Khodagholi F, et al. Pre-treatment with metformin activates Nrf2 antioxidant pathways and inhibits inflammatory responses through induction of AMPK after transient global cerebral ischemia[J]. Metab Brain Dis, 2015, 30(3):747-754. doi: 10.1007/s11011-014-9632-2
[22]	Radak D, Katsiki N, Resanovic I, et al. Apoptosis and acute brain ischemia in ischemic stroke[J]. Curr Vasc Pharmacol, 2017, 15(2):115-122. doi: 10.2174/1570161115666161104095522
[23]	Qi Z, Dong W, Shi W, et al. Bcl-2 phosphorylation triggers autophagy switch and reduces mitochondrial damage in limb remote ischemic conditioned rats after ischemic stroke[J]. Transl Stroke Res, 2015, 6(3):198-206. doi: 10.1007/s12975-015-0393-y
[24]	Wan L, Zhang L, Fan K, et al. Aloin promotes A549 cell apoptosis via the reactive oxygen species‑mitogen activated protein kinase signaling pathway and p53 phosphorylation[J]. Mol Med Rep, 2017, 16(5):5759-5768. doi: 10.3892/mmr.2017.7379 pmid: 28901386
[25]	Jin L, Piao Z H, Liu C P, et al. Gallic acid attenuates calcium calmodulin-dependent kinase II-induced apoptosis in spontaneously hypertensive rats[J]. J Cell Mol Med, 2018, 22(3):1517-1526. doi: 10.1111/jcmm.13419 pmid: 29266709
[26]	Boychuk T M, Nika O M, Tkachuk S S. The ratio of p53-proapoptotic and bcl-2 antiapoptotic activity in the hippocampus of rats with bain ischemia-reperfusion and experimental diabetes[J]. Fiziol Zh, 2016, 62(6):25-33. pmid: 29762968
[27]	Giffard R G, Yenari M A. Many mechanisms for HSP70 protection from cerebral ischemia[J]. J Neurosurg Anesthesiol, 2004, 16(1):53-61. doi: 10.1097/00008506-200401000-00010
[28]	Cheng C Y, Kao S T, Lee Y C. Ferulic acid exerts anti-apoptotic effects against ischemic injury by activating HSP70/Bcl-2- and HSP70/autophagy-mediated signaling after permanent focal cerebral ischemia in rats[J]. Am J Chin Med, 2019, 47(1):39-61. doi: 10.1142/S0192415X19500034
[29]	Xia M, Ding Q, Zhang Z, et al. Remote limb ischemic preconditioning protects rats against cerebral ischemia via HIF-1α/AMPK/HSP70 pathway[J]. Cell Mol Neurobiol, 2017, 37(6):1105-1114. doi: 10.1007/s10571-016-0444-2
[30]	Hamzei Taj S, Kho W, Riou A, et al. MiRNA-124 induces neuroprotection and functional improvement after focal cerebral ischemia[J]. Biomaterials, 2016, 91:151-165. doi: 10.1016/j.biomaterials.2016.03.025
[31]	Quirié A, Demougeot C, Bertrand N, et al. Effect of stroke on arginase expression and localization in the rat brain[J]. Eur J Neurosci, 2013, 37(7):1193-1202. doi: 10.1111/ejn.2013.37.issue-7
[32]	Liddelow S A, Guttenplan K A, Clarke L E, et al. Neurotoxic reactive astrocytes are induced by activated microglia[J]. Nature, 2017, 541(7638):481-487. doi: 10.1038/nature21029 pmid: 28099414
[33]	Kim J Y, Kim N, Yenari M A. Mechanisms and potential therapeutic applications of microglial activation after brain injury[J]. CNS Neurosci Ther, 2015, 21(4):309-319. doi: 10.1111/cns.2015.21.issue-4
[34]	Zhang X, Zhao H H, Li D, et al. Neuroprotective effects of matrix metalloproteinases in cerebral ischemic rats by promoting activation and migration of astrocytes and microglia[J]. Brain Res Bulle, 2019, 146:136-142.

组别	n	评分
假手术组	7	4.71±0.488
假手术给药组	7	4.86±0.378
模型组	8	1.63±0.517^a
模型给药组	9	2.89±1.054^b
F值		37.590
P值		< 0.001

组别	n	HSP70(/β-actin)	Bcl-2(/β-actin)	Bax(/β-actin)	Bcl-2/Bax
假手术组	4	1.06±0.068	1.10±0.086	0.94±0.202	1.21±0.176
假手术给药组	4	1.07±0.117	1.10±0.043	0.96±0.017	1.14±0.044
模型组	4	0.63±0.114^a	0.69±0.090^b	1.10±0.082	0.63±0.104^c
模型给药组	4	1.23±0.310^d	1.11±0.214^d	1.01±0.099	1.10±0.141^e
F值		8.323	10.790	1.385	17.100
P值		0.003	0.001	0.295	< 0.001

组别	n	GFAP	Iba1
假手术组	4	10.48±5.199	6.00±2.499
假手术给药组	4	6.76±5.073	4.78±1.790
模型组	4	26.27±1.996^a	20.06±3.447^a
模型给药组	4	16.53±3.218^b	12.40±4.750^b
F值		17.300	17.940
P值		< 0.001	< 0.001

Effects of Analgecine on Apoptosis and Neuroinflammation in Cerebral Ischemia-reperfusion Injury Rats

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