电针对APP/PS1小鼠皮质区磷脂酰肌醇-3激酶/糖原合成酶激酶-3α通路相关蛋白表达和老年斑沉积的影响

doi:10.3969/j.issn.1006-9771.2020.09.009

Abstract

Abstract:

Objective To observe the effects of electroacupuncture (EA) on expression of phosphatidylinositol 3 kinase/glycogen synthase kinase 3 alpha (PI3K/GSK3α) signaling pathway related proteins in cortex of APP/PS1 double transgenic mice, and the senile plaque (SP) deposit.
Methods Three-month-old male APP/PS1 mice were randomly divided into model group (n = 6) and EA group (n = 6) after gene identification, and other six wild C57 mice were as control group. EA group accepted EA at Baihui (GV20) and bilateral Shenshu (BL23) for 14 days. Then, the amount of SP in cortex, the expression of P85α and P110α, subunits of PI3K, and GSK3α and pS^21-GSK3α, were detected with Western blotting and immunohistochemistry.
Results The SP increased in the model group compared with that of the control group (P < 0.001), and it decreased in EA group compared with that of the model group ( P < 0.001). All the proteins expressed in the cytoplasm of cortical neurons. The expression of pS ^21-GSK3α, P110α and P85α decreased in the model group compared with that of the control group (P < 0.01), while the expression of GSK3α increased ( P < 0.05). The expression of pS ^21-GSK3α, P110α and P85α increased in EA group compared with that of the model group (P < 0.01), while the expression of GSK3α decreased ( P < 0.05).
Conclusion EA may adjust the PI3K/GSK3α signaling pathway, to reduce the formation and deposition of SP in APP/PS1 double-transgenic mice.

Key words: Alzheimer's disease, electroacupuncture, phosphatidylinositol 3-kinase, glycogen synthase kinase-3α, signaling pathway, mice

WU Yan-jun,WU Kai-hui,LIU Qian,WANG Yi-ying,QIU Guo-ping,XU Jin,SHENG Hua-jun,ZHU Shu-juan. Effect of Electroacupuncture on Expression of Phosphatidylinositol 3 Kinase/Glycogen Synthase Kinase 3 Alpha Pathway Related Proteins and Senile Plaque Deposition in Cortex of APP/PS1 Mice[J]. 《Chinese Journal of Rehabilitation Theory and Practice》, 2020, 26(9): 1045-1051.

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References 29

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组别	n	老年斑	GSK3α	pS²¹-GSK3α	P110α	P85α
对照组	6	0	26.350±2.367	29.800±2.622	54.150±4.113	21.180±1.472
模型组	6	6.500±2.074^a	49.590±2.457^a	22.770±0.856^a	29.340±3.555^a	16.790±2.414^b
电针组	6	2.500±1.049^c	29.030±2.259^c	27.580±1.014^c	49.320±2.268^c	21.870±0.947^c
F值		24.310	186.7000	34.770	88.940	11.740
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001

组别	n	GSK3α	pS^21-GSK3α	P110α	P85α	pS^21-GSK3α/GSK3α
对照组	6	0.987±0.283	0.983±0.114	1.160±0.114	0.912±0.230	1.068±0.310
模型组	6	1.563±0.370^a	0.521±0.118^b	0.720±0.102^b	0.450±0.234^c	0.346±0.118^b
电针组	6	1.007±0.252^d	0.861±0.182^e	1.080±0.102^f	0.942±0.213^e	0.890±0.237^e
F值		6.062	18.800	28.200	8.565	18.050
P值		0.004	< 0.001	< 0.001	< 0.001	< 0.001

Effect of Electroacupuncture on Expression of Phosphatidylinositol 3 Kinase/Glycogen Synthase Kinase 3 Alpha Pathway Related Proteins and Senile Plaque Deposition in Cortex of APP/PS1 Mice

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