丰富环境训练结合头穴丛刺对孤独症谱系障碍模型大鼠行为的影响

doi:10.3969/j.issn.1006-9771.2024.02.006

Abstract

Abstract:

Objective To observe the effect of enriched environment (EE) combined with acupuncture at head point (HA) on behavior in rats with autism spectrum disorder.
Methods Healthy female Wistar rats were given peritoneal injection of sodium valproate at 12.5 days of gestation. Twenty-four male offspring rats were randomly selected and then randomly divided into model group (n = 6), EE group (n = 6), HA group (n = 6) and EE combined with HA group (the combined group, n = 6). Six male offspring rats born from female mice injected with the same amount of saline intraperitoneally were as control group. After four weeks of treatment, all the five groups were tested with three-chamber test and marble burying test, and the sociability index, the social novelty index and the number of buried marbles were recorded. The levels of interleukin (IL)-1β and IL-6 in peripheral blood were determined by enzyme-linked immunosorbent assay (ELISA).
Results After treatment, compared with the model group, the sociability index and the social novelty index improved (P < 0.05), the number of buried marbles reduced (P < 0.05), and the levels of IL-6 and IL-1β in peripheral blood decreased in EE group, HA group and the combined group (P < 0.05); while the combined group was the best (P < 0.01).
Conclusion Both EE or acupuncture at HA could improve behavioral symptoms, and reduce the expression of inflammatory factors in rats with autism spectrum disorder. The combination of the two methods showed the best result.

Key words: autism spectrum disorder, acupuncture at head point, enriched environment, behavior, rats

CLC Number:

R749.94

MU Zichen, TANG Qiang, SHI Yunqiu, WANG Yan, ZHU Shuwei, ZHUANG Ya'nan, XU Danshuang, LI Hongyu, LI Baolong, ZHANG Chunyan, YUAN Mengke. Effect of enriched environment combined with acupuncture at head points on behavior in rats with autism spectrum disorder[J]. Chinese Journal of Rehabilitation Theory and Practice, 2024, 30(2): 176-182.

Figures/Tables 5

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References 30

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组别	n	社交能力指数	社交偏好指数
对照组	6	4.20±0.13	2.15±0.11
模型组	6	0.71±0.07^a	0.08±0.05^a
丰富环境组	6	2.10±0.10^b	1.47±0.09^b
头穴丛刺组	6	2.04±0.14^b	1.52±0.09^b
丰富环境结合头穴丛刺组	6	2.83±0.15^b,c,d	1.85±0.07^b,c,d
F值		668.302	218.728
P值		< 0.001	< 0.001

组别	n	埋珠数量
对照组	6	4.16±1.17
模型组	6	16.50±1.38^a
丰富环境组	6	12.33±0.82^b
头穴丛刺组	6	13.33±1.03^b
丰富环境结合头穴丛刺组	6	8.83±4.40^b,c,d
F值		119.716
P值		< 0.001

组别	n	IL-6	IL-1β
对照组	6	11.68±0.80	16.11±0.49
模型组	6	21.29±1.04^a	24.72±0.56^a
丰富环境组	6	16.34±1.04^b	19.98±0.75^b
头穴丛刺组	6	16.91±1.14^b	20.59±0.75^b
丰富环境结合头穴丛刺组	6	13.97±3.39^b,c,d	17.95±0.91^b,c,d
F值		84.442	126.071
P值		< 0.001	< 0.001

Effect of enriched environment combined with acupuncture at head points on behavior in rats with autism spectrum disorder

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