Objective To evaluate the effect of training for therapists based on Kirkpatrick's Four-level Model. Methods Totally 39 therapists who completed 2014 Beijing Clinical Shortage of Manpower Training Programs (Therapist) were investigated with Kirkpatrick's Four- level Model, named reaction, learning, behavior and results. Results For reaction level, the total satisfaction was high, but different with the degree of education, especially in the items of Participate in Training, Type of Cases, Organizing on Practice Training, Organizing Case Discussions and Get A Lot of Benefits from the Training (F>4.59, P<0.05). For learning level, the test scores were significantly different before and after the training (t>9.53, P<0.001). For behavior level, the self-reports of the therapists, and the reports from their department directors and colleagues all agreed that their ability improved after training (t>4.44, P<0.001). For results level, eleven therapists passed the rehabilitation therapist test, two therapists participated in some researches, and one published an article. Conclusion The therapists satisfied in the training, that may improve the ability of therapists to contribute to their organization.