CJRTP

Chinese Journal of Rehabilitation Theory and Practice >

2017 , Vol. 23 >Issue 7: 750 - 755

DOI: https://doi.org/10.3969/j.issn.1006-9771.2017.07.002

Effects of Electroacupuncture at Baihui and Shenting on Learning-memory Function and Ultrastructure in Hippocampal CA1 in Cerebral Ischmeia-reperfusion Rats

  • SONG Chang-ming ,
  • HUANG Jia ,
  • LIN Bing-bing ,
  • YANG Min-guang ,
  • ZHANG Xiu-feng ,
  • LIU Wei-lin ,
  • TAO Jing ,
  • CHEN Li-dian
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  • 1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;
    2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350122, China;
    3. Technology Innovation Platform of Fujian Rehabilitation Industry Research Institute, Fuzhou, Fujian 350122, China;
    4. Rehabilitation Research Center of Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received date: 2017-04-03

  Online published: 2017-08-07

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Abstract

ObjectiveTo observe the effect of electroacupuncture at Baihui (GV20) and Shenting (GV24) on learning-memory function and ultrastructure in hippocampal CA1 region of rats after cerebral ischmeia-reperfusion. MethodsA total of 25 male Sprague-Dawley rats were randomly divided into sham group (n=6) and operation group (n=19). The operation group was occluded the left middle cerebral arteries with modified Longa's Methods for 90 minutes and reperfused, and twelve qualified rats of them were randomly divided into model group (n=6) and electroacupuncture group (n=6), the later accepted electroacupuncture at Baihui and Shenting for seven days. They were assessed with Longa's scores, and tested with Barnes maze. Their cerebral infarct volume was tested with magnetic resonance imaging T2-weighted image. The ultrastructure of synapse in hippocampal CA1 region was observed with transmission electron microscope. ResultsCompared with the model group, the Longa's score improved (P<0.05), the infarct volume decreased (P<0.01), the average escape latency decreased (P<0.01) and the times entering the wrong hole decreased (P<0.001) in the electroacupuncture group. Under the transmission electron microscope, the number of synapse decreased in the model group, with the structure damage and vesicles sparse; compared with the model group, the number of synapse increased in the electroacupuncture group, with clear and complete structure and rich vesicles. ConclusionElectroacupuncture at Baihui and Shenting can improve the learning-memory function in rats after cerebral ischmeia-reperfusion, which may relate to improvement of synaptic plasticity and ameliorating ultrastructure in hippocampal CA1 region.

Key words： cerebral ischmeia-reperfusion; electroacupuncture; synapse; ultrastructure; learning-memory; hippocampus; rats

Cite this article

SONG Chang-ming , HUANG Jia , LIN Bing-bing , YANG Min-guang , ZHANG Xiu-feng , LIU Wei-lin , TAO Jing , CHEN Li-dian . Effects of Electroacupuncture at Baihui and Shenting on Learning-memory Function and Ultrastructure in Hippocampal CA1 in Cerebral Ischmeia-reperfusion Rats[J]. Chinese Journal of Rehabilitation Theory and Practice, 2017 , 23(7) : 750 -755 . DOI: 10.3969/j.issn.1006-9771.2017.07.002

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