Objective To investigate the effect of Jiawei Naotaifang on neuronal apoptosis and the mechanism in ovariectomized rats with cerebral ischemia. Methods Female Sprague-Dawley rats (n=40) were randomly divided into sham group (n=10), model group (n=10), estrogen group (n=10) and Jiawei Naotaifang group (n=10). The model group, estrogen group and Jiawei Naotaifang group were ovariectomized. Eleven days after ovariectomy, the estrogen group and Jiawei Naotaifang group were given estrogen and Jiawei Naotaifang respectively intragastrically for three days. 14 days after ovariectomy, the model group, estrogen group and Jiawei Naotaifang group were modeled cerebral ischemia with Langa's method. 24 hours after modeling, the apoptosis rate of neurons was detected with TUNEL, and the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (p-JNK) in hippocampus were detected with Western blotting. Results Compared with the model group, the apoptosis rates decreased in Jiawei Naotaifang group and the estrogen group (P<0.001), with more activation of ERK1/2 (P<0.01) and less activation of JNK (P<0.01).Conclusion Jiawei Naotaifang can protect neuron from apoptosis by promoting the activation of ERK1/2 and inhibiting the activation of p-JNK.
QIN Li-hua
,
LI Sheng
,
CHENG Shao-wu
,
LIU lin
,
LIU Yang
,
HUANG Juan
,
GONG Sheng-qiang
,
CHENG Cheng
,
GE Jin-wen
. Effect of Jiawei Naotaifang on Activation of Extracellular Signal-regulated Kinase 1/2 and c-Jun N-terminal Kinase in Ovariectomized Rats after Cerebral Ischemia[J]. Chinese Journal of Rehabilitation Theory and Practice, 2018
, 24(3)
: 277
-281
.
DOI: 10.3969/j.issn.1006-9771.2018.03.005
[1] Meschia JF, Bushnell C, Boden-Albala B, et al.Guidelines for the Primary Prevention of Stroke: A Statement for Healthcare Professionals from the American Heart Association/American Stroke Association[J]. Stroke, 2014, 45(12): 3754-3832.
[2] 董晓宇,翟志永,佡剑非. FLAIR血管高信号在颈动脉系统短暂性脑缺血发作的意义及相关因素分析[J]. 中国神经精神疾病杂志, 2015, 41(8): 449-454.
[3] Bauer AM, Bain MD, Rasmussen PA.Chronic cerebral ischemia: where "evidence-based medicine" fails patients[J]. World Neurosurg, 2015, 84(3): 714-718.
[4] 王巧云,刘凤,吴峰阶,等. 人参皂苷Rg1对局灶性脑缺血再灌注损伤大鼠海马p-ERK1/2与p-JNK表达的影响[J]. 中国中西医结合杂志, 2013, 33(2): 229-234.
[5] Xing L, Larsen RS, Bjorklund GR, et al.Layer specific and general requirements for ERK/MAPK signaling in the developing neocortex[J]. eLife, 2016, 5: e11123.
[6] 刘文明,蔡晓旭,赫天辉,等. 小鼠脑缺血-再灌注损伤中神经细胞BCL2及p-JNK2的表达[J]. 宁夏医科大学学报, 2016, 38(2): 148-150.
[7] 贺运河,郝晓元,葛金文. 脑泰方治疗气虚血瘀证脑梗塞临床研究[J]. 中国中医急症, 2001, 10(6): 319-320.
[8] 钟晴,童骄,钟欣池,等. 脑泰方提取物对大鼠脑缺血再灌注损伤后海马神经干细胞增殖及Notch1蛋白表达的影响[J]. 中华中医药学刊, 2016, 34(8): 1823-1826.
[9] 陈奇. 中药药效研究思路与方法[M]. 北京:人民卫生出版社, 2005: 949.
[10] Garcia JH.A reliable method to occlude a middle cerebral artery in Wistar rats[J]. Stroke, 1993, 24(9): 1423.
[11] Longa EZ, Weinstein PR, Carlson S, et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1): 84-91.
[12] 邢变枝,陈晖,张苏明. 缺血后处理对脑缺血再灌注损伤后ERK1/2和Akt磷酸化及神经细胞凋亡的影响[J]. 神经损伤与功能重建, 2012, 7(3): 175-179.
[13] Viscoli CM, Brass LM, Kernan WN, et al.A clinical trial of estrogen-replacement therapy after ischemic stroke[J]. N Engl J Med, 2001, 345(17): 1243-1249.
[14] Troy CM, Rabacchi SA, Xu Z, et al.beta-amyloid-induced neuronal apoptosis requires c-Jun N-terminal kinase activation[J]. J Neurochem, 2001, 77(1): 157-164.
[15] Gladbach A, van Eersel J, Bi M, et al. ERK inhibition with PD184161 mitigates brain damage in a mouse model of stroke[J]. J Neural Transm, 2014, 121(5): 543-547.
[16] 涂献坤,石松生,杨卫忠,等. 齐留通通过激活ERK1/2信号通路减轻大鼠脑缺血炎症反应和缺血性脑损伤[J]. 中国药理学通报, 2014, 30(10): 1441-1444.
[17] 唐强,叶涛,朱路文,等. 针康法对脑缺血大鼠神经功能和细胞外信号调节激酶1/2信号通路的影响[J]. 中国康复理论与实践, 2017, 23(1): 27-31.
[18] Zhu H, Zhu H, Xiao S, et al.Activation and crosstalk between the endoplasmic reticulum road and JNK pathway in ischemia-reperfusion brain injury[J]. Acta Neurochir (Wien), 2012, 154(7): 1197-1203.
[19] 李嘉瑶,戚基萍. 黄芩甙对缺血再灌注损伤的脑保护作用及对MAPKs的影响[J]. 哈尔滨医科大学学报, 2014, 48(2): 114-118.
[20] 林亚平,刘琴,陈楚淘,等. 针刺联合亚低温对脑缺血再灌注损伤大鼠脑组织MAPK/ERK通路及凋亡相关因子的影响[J]. 中南大学学报(医学版), 2017, 42(4): 380-388.
[21] 罗玉梅,张立羽,刘芳. 细胞外信号调节激酶的研究进展[J]. 中国生物制品学杂志, 2018, 31(1): 93-96.
[22] 吕欣欣,张瑞雪,唐吉友. ERK1/2与ROCK对话调控对脑梗死后神经血管单元的影响[J]. 国际神经病学神经外科学杂志, 2013, 40(5-6): 456-459.
[23] 黄小平,欧阳国,丁煌,等. 黄芪甲苷与三七有效成分配伍对小鼠脑缺血再灌注后神经细胞凋亡和内质网应激的影响[J]. 中草药, 2015, 46(15): 2257-2264.
[24] 向艳霞,肖凌云,张菊,等. 黄芪多糖治疗神经系统疾病的作用及其机制研究进展[J]. 中国医院药学杂志, 2016, 36(8): 687-691.
[25] 王昀,马武华. 川芎嗪对缺氧/复氧海马神经元JNK通路相关蛋白表达的影响[J]. 实用医学杂志, 2015, 31(13): 2095-2097.
